Intestinal absorption and histomorphometry of Syrian hamsters (Mesocricetus auratus) experimentally infected with Lawsonia intracellularis

Vannucci, Fábio A.; Borges, E.; Oliveira, Juliana Saes Vilaça de; Guedes, Roberto Maurício Carvalho

doi:10.1016/j.vetmic.2010.03.027

Cited by 18 publications

(20 citation statements)

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“…Since L. intracellularis is a fastidious organism and extremely difficult to isolate and propagate, mucosal homogenate challenge models have been reported in pigs using intestinal mucosa from PEaffected pigs [26][27][28]. Hamsters and mice infected with intestinal homogenates from PE-affected pigs have consistently developed subclinical PE associated with mild lesions, mainly found in the cecum and colon [15,29]. However, dehydration and profuse diarrhea were observed in hamsters inoculated with intestinal homogenate or filtrate derived from PE-affected hamsters [10,25].…”

Section: Discussionmentioning

confidence: 99%

“…The disease has been experimentally reproduced in hamsters, pigs and horses using species-specific isolates or intestinal homogenate derived from infected animals [10][11][12]. Results from cross-species experimental infections in hamsters and mice models using intestinal homogenates or porcine L. intracellularis isolates have consistently reproduced subclinical disease and mild lesions in infected animals [13][14][15]. Therefore, the bacterium seems to adapt and persist differently depending on the species origin of the isolate.…”

Section: Introductionmentioning

confidence: 99%

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Evidence of host adaptation in Lawsonia intracellularis infections

Vannucci

Pusterla

Mapes

et al. 2012

Journal of Equine Veterinary Science

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Background: Lawsonia intracellularis is the causative agent of proliferative enteropathy, an endemic disease in pigs and an emerging concern in horses. Enterocyte hyperplasia is a common lesion in every case but there are differences regarding clinical and pathological presentations among affected species. We hypothesize that host susceptibility to L. intracellularis infection depends on the species of origin of the bacterial isolate. The objective of this study was to evaluate the susceptibilities of pigs and horses to L. intracellularis infection using either a porcine or an equine isolate. Materials and methods: Twelve foals and eighteen pigs were equally divided into three groups and infected with either a porcine or an equine isolate (10 9 L. Intracellularis/challenged animal), and a saline solution (negative control group). The animals were monitored regarding clinical signs, average of daily weight gain, fecal shedding of the bacteria by PCR and humoral serological response. Results: Foals infected with the equine isolate developed moderate to severe clinical signs and maintained a lower average of weight gain compared to control foals. Fecal quantitative PCR in equine isolate-infected foals revealed higher amounts of bacterial DNA associated with longer duration of shedding compared with porcine isolateinfected foals. All four foals infected with the equine isolate demonstrated higher IgG titers in the serum compared with porcine isolate-infected foals. In the pig trial, diarrhea and seroconversion were only observed in animals infected with the porcine isolate. Pathological changes typical of proliferative enteropathy were observed in the necropsied foal infected with equine isolate and in the two necropsied pigs infected with the porcine isolate. Conclusions: Evident clinical signs, longer periods of bacterial shedding and stronger serologic immune responses were observed in animals infected with species-specific isolates. These results show that host susceptibility is driven by the origin of the isolated L. intracellularis strain.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evidence of host adaptation in Lawsonia intracellularis infections

Vannucci

Pusterla

Mapes

et al. 2012

Journal of Equine Veterinary Science

View full text Add to dashboard Cite

show abstract

“…Since L. intracellularis is a fastidious organism and extremely difficult to isolate and propagate, mucosal homogenate challenge models have been reported in pigs using intestinal mucosa from PE-affected pigs [26-28]. Hamsters and mice infected with intestinal homogenates from PE-affected pigs have consistently developed subclinical PE associated with mild lesions, mainly found in the cecum and colon [15,29]. However, dehydration and profuse diarrhea were observed in hamsters inoculated with intestinal homogenate or filtrate derived from PE–affected hamsters [10,25].…”

Section: Discussionmentioning

confidence: 99%

“…The disease has been experimentally reproduced in hamsters, pigs and horses using species-specific isolates or intestinal homogenate derived from infected animals [10-12]. Results from cross-species experimental infections in hamsters and mice models using intestinal homogenates or porcine L. intracellularis isolates have consistently reproduced subclinical disease and mild lesions in infected animals [13-15]. Therefore, the bacterium seems to adapt and persist differently depending on the species origin of the isolate.…”

Section: Introductionmentioning

confidence: 99%

Evidence of host adaptation in Lawsonia intracellularis infections

Vannucci

Pusterla

Mapes

et al. 2012

Vet Res

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BackgroundLawsonia intracellularis is the causative agent of proliferative enteropathy, an endemic disease in pigs and an emerging concern in horses. Enterocyte hyperplasia is a common lesion in every case but there are differences regarding clinical and pathological presentations among affected species. We hypothesize that host susceptibility to L. intracellularis infection depends on the species of origin of the bacterial isolate. The objective of this study was to evaluate the susceptibilities of pigs and horses to L. intracellularis infection using either a porcine or an equine isolate.Materials and methodsTwelve foals and eighteen pigs were equally divided into three groups and infected with either a porcine or an equine isolate (109L. Intracellularis/challenged animal), and a saline solution (negative control group). The animals were monitored regarding clinical signs, average of daily weight gain, fecal shedding of the bacteria by PCR and humoral serological response.ResultsFoals infected with the equine isolate developed moderate to severe clinical signs and maintained a lower average of weight gain compared to control foals. Fecal quantitative PCR in equine isolate-infected foals revealed higher amounts of bacterial DNA associated with longer duration of shedding compared with porcine isolate-infected foals. All four foals infected with the equine isolate demonstrated higher IgG titers in the serum compared with porcine isolate-infected foals. In the pig trial, diarrhea and seroconversion were only observed in animals infected with the porcine isolate. Pathological changes typical of proliferative enteropathy were observed in the necropsied foal infected with equine isolate and in the two necropsied pigs infected with the porcine isolate.ConclusionsEvident clinical signs, longer periods of bacterial shedding and stronger serologic immune responses were observed in animals infected with species-specific isolates. These results show that host susceptibility is driven by the origin of the isolated L. intracellularis strain.

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“…Pathogenicity of L. intracellularis present in the intestinal homogenated was evaluated and demonstrated in a previous study conducted by our group that used the same material (Vanucci et al 2010).…”

Section: Hamsters Sparrows and Micementioning

confidence: 99%

Infection of sparrows (Passer domesticus) and different mice strains with Lawsonia intracellularis

et al. 2013

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The susceptibility of sparrows (Passer domesticus) and strains of mice (Swiss, BALB/c, C-57 and DB-A) to Lawsonia intracellularis infection was studied. Thirty-two sparrows were inoculated with pure culture of L. intracellularis and eleven received sham inoculum. Feces were collected on -1, 7, 14 and 21 days post infection (dpi) for detection of L. intracellularis by PCR. After 21 days, all sparrows were euthanized and the tissues processed for histology and immunohistochemistry (IHC). One hundred sixty mice of four different strains (n=40, per strain) were used. For each mouse strain, 16 animals received mucosa homogenate from a pig infected with L. intracellularis, 16 received pure culture of L. intracellularis and eight animals received sham inoculum. Two control and four inoculated mice from each group were euthanized on 7, 14, 21 and 28 dpi. Sections of intestine were collected for histologic analysis and IHC and pooled feces were collected for L. intracellularis PCR. None of the sparrows had any histologic lesions characteristic of proliferative enteropathy or antigen labeling by IHC. All sparrow fecal samples were negative by PCR. All mice strains studied had histopathological lesions typical of PE and IHC labeling consistent with L. intracellularis infection, especially those animals inoculated with pure culture. The most severe lesions were observed in DB-A and Swiss mice. Fecal shedding was detected in all mice strains, with peak at 14 dpi. We conclude that sparrows do not seem to be relevant in the epidemiology of L. intracellularis. The results showed variations in the lesions among the four mice strains used. C-57 e DB-A) à infecção por L. intracellularis foi testada. Trinta e dois pardais foram inoculados com cultura pura de L. intracellularis e onze receberam placebo. As fezes foram coletadas nos dias -1, 7, 14 e 21 após a infecção (dpi) para a detecção de Lawsonia intracellularis por PCR. Após 21 dias, todos os pardais foram eutanasiados e os tecidos processados para a realização da histologia e imuno-histoquímica (IHQ

show abstract

Intestinal absorption and histomorphometry of Syrian hamsters (Mesocricetus auratus) experimentally infected with Lawsonia intracellularis

Cited by 18 publications

References 20 publications

Evidence of host adaptation in Lawsonia intracellularis infections

Evidence of host adaptation in Lawsonia intracellularis infections

Evidence of host adaptation in Lawsonia intracellularis infections

Infection of sparrows (Passer domesticus) and different mice strains with Lawsonia intracellularis

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