Intestinal absorption of medium chain fatty acids: in vivo studies in pigs devoid of exocrine pancreatic secretion

Abstract

In order to study the influence of pancreatic enzyme secretion on the intestinal absorption of mediumchain fatty acids (MCFA), three growing pigs (mean body-weight 61 kg) with ligated and severed pancreatic ducts were fitted with a permanent fistula in the duodenum and with two catheters in the portal vein and carotid artery respectively. An electromagnetic flow probe was also set up around the portal vein. A mixture of octanoic and decanoic acids, esterified as medium-chain triacylglycerols, together with maltose dextrine and a nitrogenous fraction was continuously infused for 1 h into the duodenum. Samples of blood were withdrawn from the two vessels at regular intervals of time for 8 h and further analysed for their non-esterified octanoic and decanoic acid contents. The concentrations of non-esterified octanoic and decanoic acid in the portal blood increased slowly after the beginning of each infusion, reaching about 10 times higher values than the basal level. Only 26% of octanoic acid infused in the duodenum and 27 YO of decanoic acid were recovered in the portal flow throughout each experiment. The possible mechanisms underlying the appearance of MCFA in the portal blood in the absence of pancreatic enzyme secretions and the importance of duodenal absorption of MCT in such physiological conditions have been discussed.Medium-chain triacyglycerols: Pancreatic enzyme deficiency: Pig

Portal absorption of 14C after ingestion of spiked milk with 14C-phenanthrene, 14C-benzo[a]pyrene or 14C-TCDD in growing pigs

“…Several authors found relatively constant blood flow values after ingestion of the meal (Guillot et al, 1993;Guillot et al, 1994;Lang et al, 1999). One can easily assume that portal blood flow variations in the present work were similar for all the animals and that they consequently interfered in the same way for all the three diets.…”

“…14 C meal absorption rate was calculated as: "portal absorption of 14 C / 14 C content in the meal". Blood flow per min and per kg body weight could be estimated thanks many references using growing pigs (Rérat et al, 1987;Simoes Nunes et al, 1989;Rérat et al, 1991;Rérat et al, 1992;Guillot et al, 1993;Guillot et al, 1994;Rérat et al, 1996;Santamaria et al, 1997;Lang et al, 1999;Le Floc'h et al, 1999;Vaugelade et al, 2000).…”

45Ca as a marker to assess portal absorption of calcium from milk in the growing pig

“…In fact, it is known that meal ingestion is followed by a small rise in portal blood flow during the first 1-2 postprandial hours and individual variations in pig portal blood flow have been established at between 2.8 and 5.7% [20]. Several authors found relatively constant blood flow values after ingestion of the meal [5,6]. One can easily assume that portal blood flow variations in the present work were similar for all the animals.…”

Forty years of achievement in french research on digestive physiology in the pig

“…Thus the most interesting features concern the influence of absorption kinetics on the ability of organs such as the liver or the peripheral tissues to store and release nutrients and the utilisation of dynamic aspects in the analysis of the role played by the "time" factor in protein tissue metabolism. This methodology enabled to study the digestion of some carbohydrates [167], proteins [175] and medium-chain triglycerides [73,74] in relation with hepatic metabolism and with peripheral tissue metabolism, according to their technological pretreatment (enzyme prehydrolysis...).…”