Intestinal absorption of rutin in free and conjugated forms11Abbreviations: ESI−, electrospray ionization.

Andlauer, Wilfried; Stumpf, Claudia; Fürst, Peter

doi:10.1016/s0006-2952(01)00638-4

Cited by 45 publications

(20 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intense research activity in recent years has been directed towards the absorption, metabolism and bioavailability of phenolic compounds and detailed data on the bioavailability and uptake of selected phenolic compounds has been reported (Cook & Samman, 1996;Hollman et al, 1996;Hollman et al, 1997;Böhm et al, 1998;Bourne & Rice-Evans, 1998;Lapidot et al, 1998;de Vries et al, 1998;Chesson et al, 1999;Clifford, 1999;Hollman et al, 1999;Ader et al, 2000;Andlauer et al, 2000;Azuma et al, 2000;Cassidy et al, 2000;Clifford, 2000a,b;Clifford & Scalbert, 2000;Hollman & Arts, 2000;Tomás-Barberán & Clifford, 2000a,b;Andlauer et al, 2001;Baba et al, 2001;Cao et al, 2001;Olthof et al, 2001;Adam et al, 2002).…”

Section: Structure Classification Distribution Of Phenols In Plant mentioning

confidence: 99%

Reactions of Plant Phenolics with Food Proteins and Enzymes under Special Consideration of Covalent Bonds

Kröll

Rawel

Rohn

2003

FSTR

336

273

View full text Add to dashboard Cite

Secondary plant metabolites are important native food components, which are becoming more and more interesting due to their physiological effects on human beings. One of the largest groups of these compounds is represented by plant phenols. This review summarizes the structure, classification and distribution of the phenolic compounds in plant foods, their chemistry and signification with regard to food processing and -storage as well as their physiological effects. This work focuses mainly on such reactions of the phenolic substances with proteins and enzymes that lead to covalent bonds. The derivatives formed have been characterized in terms of changes in their physicochemical and structural properties. The effect on the proteolytic in vitro digestion has been also illustrated. Further aspects reported include the influence on enzyme activity and -kinetic parameters. The different aspects of the nutritional-physiological consequences of such reactions in food and body, especially considering their significance to food science and technology are discussed.

show abstract

Section: Structure Classification Distribution Of Phenols In Plant mentioning

confidence: 99%

Reactions of Plant Phenolics with Food Proteins and Enzymes under Special Consideration of Covalent Bonds

Kröll

Rawel

Rohn

2003

FSTR

336

273

View full text Add to dashboard Cite

show abstract

“…4 Ϫ1 , meanϮS.D.] were calculated from concentration differences (DC, arteriovenous, and proximodistal), the corresponding flow rates, and the dry weight (DW) of the entire small intestine used in the experiment according to the following equation 15) :…”

Section: Reagents and Chemicalsmentioning

confidence: 99%

Quantitation Assay for Absorption and First-Pass Metabolism of Emodin in Isolated Rat Small Intestine Using Liquid Chromatography-Tandem Mass Spectrometry

Teng

Zhou

Yang

et al. 2007

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Polyphenols, a group of complex naturally occurring compounds, are widely distributed throughout the plant kingdom and thus are readily consumed by humans.1,2) As members of the polyphenol family, dietary anthraquinones have received much attention as potential protectors against a variety of human diseases, in particular cardiovascular disease and cancer.3,4) Emodin (1,3,8-trihydroxy-6-methylanthraquinone), the active components of many herbal laxatives such as aloe, senna, cascara sagrada, and rhubarb, belongs to the anthraquinone family. 5,6) Intestinal absorption is a prerequisite for a possible causal relationship between emodin intake and its proposed chemopreventive action. However, our limited knowledge of how emodin is absorbed and metabolized has hampered our understanding of its effect in the body. In addition, some similar compounds have poor bioavailability due to weak absorption and significant degradation by enzymes. 7) Furthermore, to date, information on in vivo absorption and metabolism of emodin is scarce-there are no reports of evidence of extensive first-pass metabolism of emodin in the gut-and thus intestinal handling of emodin requires further investigation to appraise its beneficial action in vivo.To address this issue, we evaluated the contribution of the small intestine to the absorption and first-pass metabolism of emodin using an isolated rat small intestine perfusion model. This model facilitates direct investigation of luminal disappearance and venous appearance of administered emodin and analyses the metabolites in both perfusate and mesenteric blood, thereby allowing the direct estimation of intestinal absorption and first-pass metabolism of this drug. 8,9) MATERIALS AND METHODS Reagents and ChemicalsEmodin and chrysophanol was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China) at the highest purity available (98%, as determined by HPLC). Emodin was dissolved in DMSO (0.1% v/v final concentration) because it is hydrophobic and poorly soluble in water. b-Glucuronidase (catalog no. G-0251) and sulfatase (catalog no. S-9754) were purchased from Sigma. All chemicals and reagents used were of analytical grade or HPLC grade.Animals Male Sprague-Dawley rats (70-110 d old) weighing between 270 and 320 g were obtained from the experimental animal center of the Fourth Military Medical University, Xi'an, China. The rats were fasted overnight with free access to water before the day of the experiment.Animal Surgery The rats were anesthetized with an intra-abdominal injection of a mixture containing 40 mg/kg phenobarbital sodium. The animals were then heparinized (90 U/kg Ϫ1 ) via the vena caudalis. During surgery, the body temperature was maintained at 37°C by a heating lamp.The small intestine was prepared as described. [10][11][12] Figure 1 schematically illustrates the vascular-luminal perfused rat intestine preparation. Briefly, the small intestine was exposed by midline incision, and a 7-to 11-cm-long segment of the intestin...

show abstract

“…Among them, HPLC methods with electrochemical [18,[20][21][22][23][24], mass spectrometric (MS) [25][26][27] and fluorescence detection [28][29][30] show adequate sensitivity. HPLC-UV-vis methods have also been reported [23,26,27,[31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Determination of St. John's wort flavonoid-metabolites in rat brain through high performance liquid chromatography coupled with fluorescence detection

Paulke

Schubert‐Zsilavecz

Wurglics

2006

Journal of Chromatography B

View full text Add to dashboard Cite

Intestinal absorption of rutin in free and conjugated forms11Abbreviations: ESI−, electrospray ionization.

Cited by 45 publications

References 44 publications

Reactions of Plant Phenolics with Food Proteins and Enzymes under Special Consideration of Covalent Bonds

Reactions of Plant Phenolics with Food Proteins and Enzymes under Special Consideration of Covalent Bonds

Quantitation Assay for Absorption and First-Pass Metabolism of Emodin in Isolated Rat Small Intestine Using Liquid Chromatography-Tandem Mass Spectrometry

Determination of St. John's wort flavonoid-metabolites in rat brain through high performance liquid chromatography coupled with fluorescence detection

Contact Info

Product

Resources

About