Intestinal absorption of the wheat allergen gliadin in rats

Yokooji, Tomoharu; Fukushima, Takahiro; Hamura, Koh; Ninomiya, Naoki; Ohashi, Ryo; Taogoshi, Takanori; Matsuo, Hiroaki

doi:10.1016/j.alit.2018.11.005

Cited by 9 publications

(5 citation statements)

References 29 publications

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“…Next, we explored the potential of ASA treatment in exacerbating the mild allergic hypersensitivity reactions induced by oral allergen challenge in sensitized mice. Several previous clinical studies reported that pre-medication with ASA amplified the appearance of IgE-mediated exercise-induced human wheat allergy symptoms [ 17 , 18 , 19 ] and suggested that ASA seems to increase allergen absorption from the gastrointestinal tract, with a resultant increase in circulating allergen levels in patients and animal models [ 19 , 20 ]. In this arm of the study, we tested the effects of two forms of aspirins; ASA in 50% EtOH, and L-ASA in 5% EtOH administered orally 30 min before allergen challenge.…”

Section: Resultsmentioning

confidence: 99%

“…The different effects noticed in our study between ASA and L-ASA may be related to the known stimulatory effect of ASA on the gastrointestinal mucosa, particularly on the gastric mucosa [ 31 , 32 ], compared with the milder gastrotoxicity of L-ASA [ 33 ]. ASA is also known to enhance intestinal ovalbumin absorption in rodents by inducing intestinal barrier disruption and increasing paracellular permeability [ 20 , 34 ]. That the strongest hypersensitivity and clearest symptoms were noted with simultaneous administration of allergen and ASA ( Figure 4 ) strongly suggests that ASA acts in our model as a transitory irritant of the mucosal lining of gastrointestinal tract.…”

Section: Discussionmentioning

confidence: 99%

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A Murine Model of Food Allergy by Epicutaneous Adjuvant-Free Allergen Sensitization Followed by Oral Allergen Challenge Combined with Aspirin for Enhanced Detection of Hypersensitivity Manifestations and Immunotherapy Monitoring

et al. 2023

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Food allergy is one of the major existing health problems, but no effective treatment is available. In the current work, a murine model that closely mimics pathogenesis of human food allergy and its quantifiable diagnostic parameter design, even for mild hypersensitivity reactions, were established. BALB/c mice were epicutaneously sensitized with 1 mg chicken egg ovomucoid (OVM) or cow’s milk casein, free of adjuvants, five times a week for two consecutive weeks. Eleven days later, allergen-specific IgG1 and IgE in serum were measured by ELISA. On day 25, 20 mg OVM or 12 mg α-casein was administered orally, and allergic reactions such as the fall in rectal temperature, symptom scores during 90–120 min, serum mast cell protease-1 and cytokine levels were monitored. The detection of mild allergic reactions due to adjuvant-free allergen sensitization and oral allergen challenge routes was amplified by the combination of oral allergen and aspirin administration simultaneously or aspirin administration within 15–30 min before an allergen challenge. Quantification of the maximum symptom score and the frequency of symptoms during the monitoring period improved evaluation accuracy of food allergy signals. Based on these results, efficacy of casein oral immunotherapy for cow’s milk allergies, which are generally difficult to detect, was monitored adequately.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Murine Model of Food Allergy by Epicutaneous Adjuvant-Free Allergen Sensitization Followed by Oral Allergen Challenge Combined with Aspirin for Enhanced Detection of Hypersensitivity Manifestations and Immunotherapy Monitoring

et al. 2023

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“…Yokooji et al detected plasma gliadin after ingestion of 50 mg of gluten or 100 mg/ kg of gliadin combined with aspirin intake in rats by using ELISA specific for ω5-gliadin. 5 They estimated that a 50 mg gluten dose in rats corresponds to 1.3 g in humans. Considering these results, we conducted a challenge test with a lower amount of gluten in combination with exercise or aspirin intake to determine the effects of these cofactors on the gastrointestinal gliadin absorption.…”

Section: Cofactors Of Wheat-dependent Exercise-induced Anaphylaxis In...mentioning

confidence: 99%

Cofactors of wheat‐dependent exercise‐induced anaphylaxis increase gastrointestinal gliadin absorption by an inhibition of prostaglandin production

Morita

Chinuki

Kohno

et al. 2022

Clin Experimental Allergy

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“…Dietary proteins are primarily broken down by proteolytic enzymes and peptidases within the gastrointestinal tract when ingested by humans or animals. However, a minor proportion of intact allergens or their fragments escape enzymatic hydrolysis, allowing them to be absorbed in the intestine [ 7 , 8 ]. The varying resistance to enzymatic degradation among different dietary proteins strongly correlates with their overall allergenicity [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

A Quantity-Dependent Nonlinear Model of Sodium Cromoglycate Suppression on Beta-Conglycinin Transport

Zheng,

Han,

Chen

et al. 2024

IJMS

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Understanding the transport mechanism is crucial for developing inhibitors that block allergen absorption and transport and prevent allergic reactions. However, the process of how beta-conglycinin, the primary allergen in soybeans, crosses the intestinal mucosal barrier remains unclear. The present study indicated that the transport of beta-conglycinin hydrolysates by IPEC-J2 monolayers occurred in a time- and quantity-dependent manner. The beta-conglycinin hydrolysates were absorbed into the cytoplasm of IPEC-J2 monolayers, while none were detected in the intercellular spaces. Furthermore, inhibitors such as methyl-beta-cyclodextrin (MβCD) and chlorpromazine (CPZ) significantly suppressed the absorption and transport of beta-conglycinin hydrolysates. Of particular interest, sodium cromoglycate (SCG) exhibited a quantity-dependent nonlinear suppression model on the absorption and transport of beta-conglycinin hydrolysates. In conclusion, beta-conglycinin crossed the IPEC-J2 monolayers through a transcellular pathway, involving both clathrin-mediated and caveolae-dependent endocytosis mechanisms. SCG suppressed the absorption and transport of beta-conglycinin hydrolysates by the IPEC-J2 monolayers by a quantity-dependent nonlinear model via clathrin-mediated and caveolae-dependent endocytosis. These findings provide promising targets for both the prevention and treatment of soybean allergies.

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Intestinal absorption of the wheat allergen gliadin in rats

Cited by 9 publications

References 29 publications

A Murine Model of Food Allergy by Epicutaneous Adjuvant-Free Allergen Sensitization Followed by Oral Allergen Challenge Combined with Aspirin for Enhanced Detection of Hypersensitivity Manifestations and Immunotherapy Monitoring

A Murine Model of Food Allergy by Epicutaneous Adjuvant-Free Allergen Sensitization Followed by Oral Allergen Challenge Combined with Aspirin for Enhanced Detection of Hypersensitivity Manifestations and Immunotherapy Monitoring

Cofactors of wheat‐dependent exercise‐induced anaphylaxis increase gastrointestinal gliadin absorption by an inhibition of prostaglandin production

A Quantity-Dependent Nonlinear Model of Sodium Cromoglycate Suppression on Beta-Conglycinin Transport

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