Intestinal absorption of two potential polymeric food additives in man

Walson, Philip D.; Carter, D.E.; Ryerson, B.A.; Halladay, Steven C.; Parkinson, Thomas M.

doi:10.1016/0015-6264(79)90282-7

Cited by 10 publications

(3 citation statements)

References 6 publications

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“…Thus, nonabsorbable reference markers are usually required in many intestinal absorption studies, as changes in concentration of absorbate relative to that of marker permit quantitation of absorbate movement (24). Poly R-478, a stable polymeric food dye, has been proven to be an effective and easily measured nonabsorbable marker for use in both animal and human intestinal absorption studies (24)(25)(26). In this study, we estimated the amount of dietary FA that remained in intestinal content and feces with the aid of poly R-478 after FA, FAA, and FAXn were fed to rats.…”

Section: Introductionmentioning

confidence: 99%

Digestion and Absorption of Ferulic Acid Sugar Esters in Rat Gastrointestinal Tract

Zhao

Egashira

Sanada

2003

J. Agric. Food Chem.

111

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We estimated the absorption site and absorptivity of ferulic acid (FA) and its sugar esters, namely 5-O-feruloyl-l-arabinofuranose (FAA) and feruloyl-arabinoxylan (FAXn), in rats on the basis of their recovery in intestinal content and feces by comparing the values with those of a nonabsorbable marker, poly R-478. The results indicated that free FA was absorbed almost completely before reaching cecum. About 40% of dietary FAA was absorbed in rat foregut and 57% disappeared in the cecum. In contrast, about 67% of the FA moiety in FAXn was released and then disappeared predominantly in the hindgut. These results suggested that the existing form of FA in diets affects its absorptivity, its absorption site, and its ensuing fate in the gastrointestinal tract. Those ingested FAs esterified with saccharides; especially, polysaccharides have to transit the hindgut where FA might be released and then absorbed and/or degraded by microflora in lumen. Such microbial degradation may be an important factor affecting the bioavailability of dietary FA.

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Section: Introductionmentioning

confidence: 99%

Digestion and Absorption of Ferulic Acid Sugar Esters in Rat Gastrointestinal Tract

Zhao

Egashira

Sanada

2003

J. Agric. Food Chem.

111

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“…The recovery of greater than 100% of the Poly R-478 and the considerable variation in the recovery was most likely due to small losses of volume in the extraction and centrifugation steps that were magnified by the dilution of the samples to bring the color content into the linear range of the spectrophotometer. Although the current studies have not attempted to recover Poly R-478 from gastric contents, both animal and human studies (11,12) document the complete recovery of Poly R-478 after passage through the entire gastrointestinal tract. These findings suggest that Poly R-478 continues to be an effective NAM even when food solids are present in the stomach or intestine.…”

Section: Discussionmentioning

confidence: 91%

“…Poly R-478 (MW -40,000) is an anthrapyridone chromophore linked to an acetylated polyamino-ethylene-sodium ethylene sulfonate copolymer backbone. Adult, small animal (11), and human studies (12) have shown that Poly R-478 is nonabsorbable from the gastrointestinal tract and nontoxic at doses up to to g/kg. It was also shown to be highly water soluble (up to 20 weight %) and thermally and photochemically stable.…”

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confidence: 99%

Comparison of Nonabsorbable Markers Poly R‐478 and [¹⁴C]PEG‐4,000 for Use in Developmental Absorption Studies

Stahl,

Fayer,

Ling

et al. 1991

J. pediatr. gastroenterol. nutr.

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SummaryTo determine the utility of Poly R‐478, a stable, polymeric dye (MW, 40,000), as a nonabsorbable marker for studies in the developing small intestine, it was validated by comparison to carbon 14‐labeled polyethylene glycol, MW 4,000 ([14C]PEG‐4,000) in rats from 14 to 40 days of age. The recovery and quantification of Poly R‐478 from biological samples is simple and rapid compared to other nonradioactive nonabsorbable markers. In 40‐day‐old rats simultaneously given Poly R‐478 and [14C]PEG, total recoveries were similar, the percentages of the Poly R‐478 dose and the [14C]PEG dose recovered per segment were identical, and taurocholate absorption rates calculated using each marker were comparable. Recovery of Poly R‐478 from flushed intestinal segments, determined by a one‐step extraction, was equivalent to that of [14C]PEG in 40‐day‐old rats (96.5 ± 3.7% versus 102.7 ± 10.1%; NS) but was superior to that of [14C]PEG in 21‐day‐old rats (98.0 ± 6.2% versus 63.4 ± 5.5%; p < 0.001) and 14‐day‐old rats (97.8 ± 6.7% versus 56.7 ± 12.6%; p < 0.001). Within each age group, the distribution of Poly R‐478 within the intestine was similar to that of [14C]PEG. In addition, total taurocholate absorption in the presence of Poly R‐478 was comparable to that in the presence of [14C]PEG. Complete recovery of Poly R‐478 from a suspension of liver particles and from mixtures with two commercial infant formulas was demonstrated in vitro, suggesting that Poly R‐478 may be used when foods are present in the intestine. Poly R‐478 is an effective and easily measured nonabsorbable marker and allows for development absorption studies utilizing two radiolabeled substrates.

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Polymeric Dyes: Useful Nonabsorbable Reference Markers for Intestinal Perfusion Studies in Animals

Dupas

Moreau

Hofmann

1985

Journal of Pharmaceutical Sciences

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Intestinal absorption of two potential polymeric food additives in man

Cited by 10 publications

References 6 publications

Digestion and Absorption of Ferulic Acid Sugar Esters in Rat Gastrointestinal Tract

Digestion and Absorption of Ferulic Acid Sugar Esters in Rat Gastrointestinal Tract

Comparison of Nonabsorbable Markers Poly R‐478 and [¹⁴C]PEG‐4,000 for Use in Developmental Absorption Studies

Polymeric Dyes: Useful Nonabsorbable Reference Markers for Intestinal Perfusion Studies in Animals

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Intestinal absorption of two potential polymeric food additives in man

Cited by 10 publications

References 6 publications

Digestion and Absorption of Ferulic Acid Sugar Esters in Rat Gastrointestinal Tract

Digestion and Absorption of Ferulic Acid Sugar Esters in Rat Gastrointestinal Tract

Comparison of Nonabsorbable Markers Poly R‐478 and [14C]PEG‐4,000 for Use in Developmental Absorption Studies

Polymeric Dyes: Useful Nonabsorbable Reference Markers for Intestinal Perfusion Studies in Animals

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Comparison of Nonabsorbable Markers Poly R‐478 and [¹⁴C]PEG‐4,000 for Use in Developmental Absorption Studies