Intestinal alkaline phosphatase in patients with liver disease

Barakat, M. Yu.; Grubb, Maria C. Mejia de; Goyal, Raj K.; Hersh, Theodore

doi:10.1007/bf02235168

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…3 ). Toxic lesions of the liver, infectious hepatitis [58][59][60] or seriously congested liver [57,61] show similar pictures. These observations demonstrate that a simple enzyme histochemical reaction for alkaline phosphatase indicates a functional disturbance of the liver in biopsy or autoptic liver tissue.…”

Section: Applications Of Enzyme Histochemistry As a Tool In Diagnostimentioning

confidence: 67%

Current Concepts of Enzyme Histochemistry in Modern Pathology

Meier‐Ruge

Bruder²

2008

Pathobiology

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Enzyme histochemistry serves as a link between biochemistry and morphology. It is based on metabolization of a substrate provided to a tissue enzyme in its orthotopic localization. Visualization is accomplished with an insoluble dye product. It is a sensitive dynamic technique that mirrors even early metabolic imbalance of a pathological tissue lesion, combined with the advantage of histotopographic enzyme localization. With the advent of immunohistochemistry and DNA-oriented molecular pathology techniques, the potential of enzyme histochemistry currently tends to be underrecognized. This review aims to draw attention to the broad range of applications of this simple, rapid and inexpensive method. Alkaline phosphatase represents tissue barrier functions in brain capillaries, duodenal enterocyte and proximal kidney tubule brush borders. Decrease in enzyme histochemical alkaline phosphatase activity indicates serious functional impairment. Enzyme histochemical increase in lysosomal acid phosphatase activity is an early marker of ischemic tissue lesions. Over the last four decades, acetylcholinesterase enzyme histochemistry has proven to be the gold standard for the diagnosis of Hirschsprung disease and is one of the most commonly applied enzyme histochemical methods today. Chloroacetate esterase and tartrate-resistant phosphatase are both resistant to formalin fixation, EDTA decalcification and paraffin embedding. Early enzyme histochemical insight into development of a pathologic tissue lesion and evaluation of function and vitality of tissue enhance our understanding of the pathophysiology of diseases. In this process, enzyme histochemistry constitutes a valuable complement to conventional histology, immunohistochemistry and molecular pathology for both diagnostic and experimental pathology.

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Section: Applications Of Enzyme Histochemistry As a Tool In Diagnostimentioning

confidence: 67%

Current Concepts of Enzyme Histochemistry in Modern Pathology

Meier‐Ruge

Bruder²

2008

Pathobiology

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“…The elevation of liver and intestinal isoen zymes, for instance, is a frequent finding in primary or secondary pathological involve ment of the liver and in biliary tract lesions [4], Similarly, in a variety of bone diseases, an elevation of the bone isoenzyme has been useful in diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Serum Alkaline Phosphatase Isoenzymes in an Arab Population with Non-Insulin-Dependent Diabetes mellitus

Abdella¹,

Barakat²,

Al-Sabbagh³

et al. 1990

Med Principles Pract

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Total serum alkaline phosphatase and its liver, bone and intestinal isoenzymes were estimated in 62 Arab subjects with non-insulin-dependent diabetes mellitus and compared with the levels in 64 healthy controls. A significant elevation of total alkaline phosphatase was found in the diabetic patients (126IU/1) compared to the controls (79 IU/1). The level of the liver alkaline phosphatase isoenzyme fraction was also higher in the diabetic group (84 IU/1) than in the controls (64 IU/1). In addition, the value for the bone isoenzyme fraction, which was the predominant fraction in 16 diabetics and 21 controls, was significantly higher in diabetics (122 IU/1) than in controls (31 IU/1). However, the intestinal alkaline phosphatase band, which was present in 12 diabetics (54IU/1) and 7 healthy controls (42 IU/1), was not significantly different. Both the diabetics and controls with this band had either blood group·or B. Total alkaline phosphatase and the bone isoenzyme were found to correlate positively with fasting blood glucose. Thus, although total alkaline phosphatase and its bone and liver isoenzyme fractions are elevated in diabetic subjects, the level of the intestinal fraction seems to be independent of the diabetic state.

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Intestinal origin alkaline phosphatase activity in plasma for differential diagnosis of jaundice.

Kuwana

Rosalki

1991

J Clin Pathol

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Intestinal origin alkaline phosphatase activity (ALP) in plasma was measured by a sensitive immunocapture assay in 104 jaundiced patients-84 with intrahepatic and 20 with post-hepatic jaundice. Increased enzyme activities were observed in those with intrahepatic disease and subnormal values in those with post-hepatic disease. At a discriminant level intestinal origin ALP showed a diagnostic sensitivity of 77% for intrahepatic cholestasis, with a diagnostic accuracy of 75% for its differentiation from post-hepatic jaundice. This diagnostic accuracy is not as good as that derived with other techniques -for example, imaging-and the technique is therefore not recommended as a supplement or replacement.

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Intestinal alkaline phosphatase in patients with liver disease

Cited by 3 publications

References 12 publications

Current Concepts of Enzyme Histochemistry in Modern Pathology

Current Concepts of Enzyme Histochemistry in Modern Pathology

Serum Alkaline Phosphatase Isoenzymes in an Arab Population with Non-Insulin-Dependent Diabetes mellitus

Intestinal origin alkaline phosphatase activity in plasma for differential diagnosis of jaundice.

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