Intestinal barrier and mucosal immunity in broilers, Thai Betong, and native Thai Praduhangdum chickens

Theerawatanasirikul, Sirin; Koomkrong, Nunyarat; Kayan, Autchara; Boonkaewwan, Chaiwat

doi:10.3906/vet-1609-58

Cited by 11 publications

(13 citation statements)

References 22 publications

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“…Mucin coats the gastrointestinal tract, and it is accepted as the first-line host defense. These cells in the intestinal mucosa provide lubricity via mucin which also acts as a protective barrier against pathogenic microorganisms [ 52 ]. Gao et al [ 46 ] reported that the increase in the GCC may be related to the increase in the CD, which reflects epithelial regeneration.…”

Section: Discussionmentioning

confidence: 99%

Dietary encapsulated essential oil mixture influence on apparent nutrient digestibility, serum metabolic profile, lymphocyte histochemistry and intestinal morphology of laying hens

et al. 2022

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The study was aimed to evaluate the effects of a mixture of encapsulated essential oils addition on nutrient digestion, serum biochemical parameters, peripheral blood alphanaphthyl acetate esterase (ANAE), and acid phosphatase (ACP-ase) positive lymphocyte ratios and intestinal morphology in laying hens.Methods: A total of 320 laying hens of 48-wk-old were randomly allotted into 4 treatment groups with 10 replicates of 8 birds in each replicate. The birds were fed a basal diet (Control) or the diet added with mixture of EOs (which consist of eugenol, nerolidol, piperine, thymol, linalool, and geraniol) at 50, 100, and 200 mg/kg for period of 84 days.Results: The addition of EOs at 100 or 200 mg/kg increased the dry matter, organic matter, and crude protein digestion as compared to Control. The addition of all doses of EOs did not affect serum GGT, ALT, and P but increased serum AST concentration. The addition of 200 mg/kg EOs increased serum creatinine, while 100 mg/kg decreased Ca concentration. The addition of 100 and 200 mg/kg EOs generally improved ANAE and ACP-ase positive peripheral blood lymphocyte ratios and intestinal morphology. Conclusion:It can be concluded that, the addition of 100 or 200 mg/kg encapsulated essential oils generally increased apparent nutrient digestion and serum AST concentration, improved ANAE and ACP-ase positive peripheral blood lymphocytes and intestinal morphology in laying hens.

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Section: Discussionmentioning

confidence: 99%

Dietary encapsulated essential oil mixture influence on apparent nutrient digestibility, serum metabolic profile, lymphocyte histochemistry and intestinal morphology of laying hens

et al. 2022

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“…Claudin1, Claudin5, and Claudin16 are interstitially located in the intestinal mucosal epithelial cells and together form a tight junction in chickens (Ozden, et al, 2010;Theerawatanasirikul, et al, 2017). In contrast, Claudin5 and JAM2 proteins are not only expressed in the mucosal epithelium, but also in the vascular endothelial cells of mice (Martin-Padura, et al, 1998;Morita, et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

<i>Lactobacillus reuteri</i> Enhances the Mucosal Barrier Function against Heat-killed <i>Salmonella</i> Typhimurium in the Intestine of Broiler Chicks

et al. 2020

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Salmonella Typhimurium (ST) infection in chickens inhibits their growth and can lead to food-borne diseases in humans. Probiotics are expected to enhance the function of host intestinal barrier against pathogen infection. The aim of our study was to determine the effect of viable Lactobacillus reuteri (LR) on the response of the mucosal barrier function to antigen stimulation in broiler chicks. Day-old male (n＝8) and female (n＝4) broiler chicks were orally administered either 1×10 8 LR or a water-only control, every day for 7 days. After 7 days, either 1×10 8 heatkilled ST (k-ST), or a buffer-only control, was administered via intra-cardiac injection. The ileum and cecum were collected 3 h post-injection, and paraffin sections were prepared for either mRNA extraction (males), or gut permeability tests (females). Villus and crypt lengths were determined via histological analysis. Real-time PCR was used to calculate expression levels of Toll-like receptors (TLRs), pro-inflammatory cytokines, anti-inflammatory cytokines, avian β-defensins, and tight-junction-associated molecules. Gut permeability was assessed using the inverted intestine method. We found that (1) expression of TLR2-1, TLR21, TGF-β2 and TGF-β3 were reduced following k-ST stimulation, but were unaffected by LR-treatment; (2) oral administration of LR led to increased Claudin1, Claudin5, ZO2, and JAM2 expression following k-ST stimulation; (3) cecal permeability was reduced by co-treatment with LR and k-ST, but not by treatment with LR or k-ST alone. These results suggest that LR, as used in this study, may enhance the intestinal mucosal physical barrier function, but not the expression of other immunerelated factors in newly hatched chicks.

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“…Epithelial cells are protected from physical damage by intraluminal substances and block the invasion of pathogenic bacteria. To provide continuous protection, goblet cells must constantly be renewed [ 25 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

The effect of cashew leaf extract on small intestine morphology and growth performance of Jawa Super chicken

2018

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Aim:This research aimed to study the effect of leaf extract of cashew as a bioactive compound in feed on the morphology of the small intestine in Jawa Super chicken (Gallus gallus domesticus).Materials and Methods:This study used 72 1-day-old Jawa Super chicks reared for a further 16 days. We used a randomized complete design, in which basal feed was supplemented with ethanolic extract of cashew leaves at 0 g/kg feed (control), 1.25 g/kg feed (P1), 2.5 g/kg feed (P2), 5 g/kg feed (P3), 10 g/kg feed (P4), and 20 g/kg feed (P5). Parameters observed included growth performance, chicken morphometry, and morphology of the small intestine, comprising the length and width of the villi, the depth of the crypt, and the number and size of goblet cells in the duodenum, jejunum, and ileum. Data analysis was conducted using one-way ANOVA followed by Duncan’s test, with significance defined as p<0.05.Results:Ethanolic extract of cashew leaf significantly increased body weight, feed efficiency, body morphometry, villus length, crypt depth, number of goblet cells, and extent of goblet cell area of the small intestine at 16 days. The morphological results from the small intestine showed that P4 and P5 were significantly better than control.Conclusions:Cashew leaf ethanolic extract mixed with 10 g/kg basal feed is effective as a natural feed supplement for Jawa Super chickens.

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Intestinal barrier and mucosal immunity in broilers, Thai Betong, and native Thai Praduhangdum chickens

Cited by 11 publications

References 22 publications

Dietary encapsulated essential oil mixture influence on apparent nutrient digestibility, serum metabolic profile, lymphocyte histochemistry and intestinal morphology of laying hens

Dietary encapsulated essential oil mixture influence on apparent nutrient digestibility, serum metabolic profile, lymphocyte histochemistry and intestinal morphology of laying hens

<i>Lactobacillus reuteri</i> Enhances the Mucosal Barrier Function against Heat-killed <i>Salmonella</i> Typhimurium in the Intestine of Broiler Chicks

The effect of cashew leaf extract on small intestine morphology and growth performance of Jawa Super chicken

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