Intestinal Barrier Disruption in Ileal Pouchitis After Ileal Pouch-Anal Anastomosis in a Rat Model

Li, Kaiyu; Wang, Jianlin; Xu, Yiting; Gao, Sen-Yang; Zhang, Yingying; He, Anbang; Liu, Gang

doi:10.1097/mib.0000000000001129

Cited by 8 publications

(5 citation statements)

References 27 publications

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“…In turn, intestinal barrier dysfunction may lead to the extension of the local small intestinal inflammatory response to a more generalized systemic response; thereby inhibiting recovery in patients with this disease . Indeed, GB dysfunction has been shown to be involved in many diseases, including ileal pouchitis, nonalcoholic steatohepatitis, and heart failure . A study by Snoek et al .…”

Section: Introductionmentioning

confidence: 99%

Alterations in the gut barrier and involvement of Toll‐like receptor 4 in murine postoperative ileus

Lin

Zhang

Shen

et al. 2018

Neurogastroenterology Motil

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Section: Introductionmentioning

confidence: 99%

Alterations in the gut barrier and involvement of Toll‐like receptor 4 in murine postoperative ileus

Lin

Zhang

Shen

et al. 2018

Neurogastroenterology Motil

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“…The mechanical barrier, formed by neighboring epithelial cells, excludes microbial pathogen-associated molecular patterns, toxins, and foreign antigens [ 73 ]. TJ proteins, such as ZO-1 and OCLN, are essential components of the intestinal mechanical barrier [ 74 ]. Previous studies have reported decreased expression of TJ proteins in DSS-induced colitis [ 75 ].…”

Section: Discussionmentioning

confidence: 99%

Bacillus siamensis Targeted Screening from Highly Colitis-Resistant Pigs Can Alleviate Ulcerative Colitis in Mice

Fang,

Liu,

et al. 2024

Research

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Ulcerative colitis (UC) is often accompanied by intestinal inflammation and disruption of intestinal epithelial structures, which are closely associated with changes in the intestinal microbiota. We previously revealed that Min pigs, a native Chinese breed, are more resistant to dextran sulfate sodium (DSS)-induced colitis than commercial Yorkshire pigs. Characterizing the microbiota in Min pigs would allow identification of the core microbes that confer colitis resistance. By analyzing the microbiota linked to the disease course in Min and Yorkshire pigs, we observed that Bacillus spp. were enriched in Min pigs and positively correlated with pathogen resistance. Using targeted screening, we identified and validated Bacillus siamensis MZ16 from Min pigs as a bacterial species with biofilm formation ability, superior salt and pH tolerance, and antimicrobial characteristics. Subsequently, we administered B. siamensis MZ16 to conventional or microbiota-deficient BALB/c mice with DSS-induced colitis to assess its efficacy in alleviating colitis. B. siamensis MZ16 partially counteracted DSS-induced colitis in conventional mice, but it did not mitigate DSS-induced colitis in microbiota-deficient mice. Further analysis revealed that B. siamensis MZ16 administration improved intestinal ecology and integrity and immunological barrier function in mice. Compared to the DSS-treated mice, mice preadministered B. siamensis MZ16 exhibited improved relative abundance of potentially beneficial microbes ( Lactobacillus , Bacillus , Christensenellaceae R7 , Ruminococcus , Clostridium , and Eubacterium ), reduced relative abundance of pathogenic microbes ( Escherichia-Shigella ), and maintained colonic OCLN and ZO-1 levels and IgA and SIgA levels. Furthermore, B. siamensis MZ16 reduced proinflammatory cytokine levels by reversing NF-κB and MAPK pathway activation in the DSS group. Overall, B. siamensis MZ16 from Min pigs had beneficial effects on a colitis mouse model by enhancing intestinal barrier functions and reducing inflammation in a gut microbiota-dependent manner.

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“…As a part of the intestinal immune system, the intestinal barrier is an effective protective barrier to prevent pathogenic microorganisms . More and more studies have shown that puerarin and tryptophan or its metabolites participate in maintaining intestinal homeostasis. , Studies have shown that DSS can effectively cause severe intestinal barrier damage and IBD. Therefore, DSS was used to build an intestinal barrier injury model in rats in our study.…”

Section: Discussionmentioning

confidence: 99%

“…However, mucin decreased significantly in the DSS group, suggesting that DSS just promoted the expression of VAMP8. It is well-known that DSS destroys the integrity of intestinal epithelial cells and increases intestinal permeability . Furthermore, VAMP8 is a protein associated with the cell membrane and involved in the formation of vesicles and the secretion of mucin .…”

Section: Discussionmentioning

confidence: 99%

“…It is well-known that DSS destroys the integrity of intestinal epithelial cells and increases intestinal permeability. 28 Furthermore, VAMP8 is a protein associated with the cell membrane and involved in the formation of vesicles and the secretion of mucin. 49 Therefore, we speculated that the injured body enhanced the barrier function of the intestinal epithelial cell membrane and promoted the secretion of mucin by goblet cells by increasing the expression of VAMP8, which was mainly to maintain the stability of the intestinal environment and prevent further expansion of DSS damage.…”

Section: ■ Discussionmentioning

confidence: 99%

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Enhanced Intestinal Barriers by Puerarin in Combination with Tryptophan

You

Ding

et al. 2021

J. Agric. Food Chem.

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The intestinal barrier is essential for maintaining human intestinal health. The growing number of studies has shown that both puerarin and tryptophan and its metabolites have a beneficial effect on the intestinal barrier. This study aims at the combination of puerarin and tryptophan or its metabolites for improving the intestinal barrier. In our study, 40 female Sprague− Dawley rats were randomly divided into five groups (n = 8) for a 4-week experiment and dextran sodium sulfate was used to induce an intestinal barrier injury in rats. Our results showed that puerarin combined with tryptophan or its metabolites (indole-3-propionic acid, IPA) improved the intestinal barrier by enhancing the mucus layer barrier, which was mainly achieved by increasing the number of goblet cells and promoting the secretion of MUC2. Both TRPM5 and VAMP8 promoted MUC2 secretion in goblet cells through exocytosis, but their mechanisms of action are different. In our study, we found that puerarin and tryptophan showed different effects on TRPM5 and VAMP8, respectively. Puerarin enhances the expression of TRPM5, and tryptophan inhibits the expression of TRPM5; however, puerarin and tryptophan have no significant effect on the expression of VAMP8.

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Intestinal Barrier Disruption in Ileal Pouchitis After Ileal Pouch-Anal Anastomosis in a Rat Model

Abstract: The intestinal barrier was disrupted in this rat model of pouchitis. Increased proinflammatory and decreased anti-inflammatory factors aggravated the intestinal barrier damage. Antibiotics may ameliorate this process.

Cited by 8 publications

References 27 publications

Alterations in the gut barrier and involvement of Toll‐like receptor 4 in murine postoperative ileus

Alterations in the gut barrier and involvement of Toll‐like receptor 4 in murine postoperative ileus

Bacillus siamensis Targeted Screening from Highly Colitis-Resistant Pigs Can Alleviate Ulcerative Colitis in Mice

Enhanced Intestinal Barriers by Puerarin in Combination with Tryptophan

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