Intestinal D-glucose and L-alanine transport in Japanese quail (Coturnix coturnix)

Abstract

The mechanisms involved in D-glucose and amino acid transport in the intestine of birds are still not clear. In chickens, D-glucose and amino acid absorption occurs via carrier-mediated transport, but in wild birds a passive paracellular mechanism seems to be the predominant pathway. The purpose of this work was to determine the existence of carrier-mediated sodium cotransport of D-glucose and L-alanine in the small intestine of Japanese quail (Coturnix coturnix), a granivorous bird. Intestinal transport was determined by changes in the short-circuit current (Isc), proportional to ion transmembrane flux, in the middle segment of the intestine of Japanese quail with a Ussing chamber. D-Glucose produced an increase of the Isc, and this effect was reverted by phloridzin, indicating the presence of a D-glucose transport mediated by the sodium/glucose cotranspoter 1. Addition of L-alanine also produced an increase of the Isc. We concluded that there is carrier-mediated cotransport of D-glucose and L-alanine with sodium in the small intestine of the Japanese quail.

Effects of Dietary Glucose Oxidase Supplementation on the Performance, Apparent Ileal Amino Acids Digestibility, and Ileal Microbiota of Broiler Chickens

“…Similarly, Wu et al found that the decrease in the pH of intestinal content caused by dietary GOD addition activated intestinal digestive enzymes, such as pepsin and chymotrypsin, promoted the digestion and absorption of nutrients, and improved nutrient metabolism rate of broilers [7]. Additionally, glucose and some amino acids shared the same transporters [36][37][38]. Our result of decrease in intestinal glucose caused by GOD addition might cause the increase in the uptake of some amino acids in the intestine of broilers.…”

Dietary inulin alters the intestinal absorptive and barrier function of piglet intestine after weaning

“…The brush border membrane depolarization and rise in cytosolic sodium concentration stimulate the Na + -K + ATPase, leading to an increase in the net flux of sodium from luminal to the serosal side. These events resulted in an increase in the I sc (Wright et al, 1994;Garcia-Amado et al, 2005). Furthermore, the current study showed that I sc as an indicator of glucose transport increased with age in the postnatal period; suggesting that there may be some degree of interaction between nutrient intake and gut development during the immediate post-weaning period.…”

Evaluation of the chicory inulin efficacy on ameliorating the intestinal morphology and modulating the intestinal electrophysiological properties in broiler chickens

“…The net movements of ions are responsible for the electrical current across the epithelium. There are two mechanisms for the absorption of d ‐glucose in the intestines; paracellular (passive diffusion) and transcellular transport (Garcia‐amado et al, 2005). In the former, no input of energy is required, while for the transcellular transport, energy is required for the absorption of glucose mediated by the sodium/glucose cotransporter‐1, SGLT1 and amino acids via carrier proteins in the apical and basolateral membranes of the epithelial cells (Pappenheimer, 1993).…”

“…The brush border membrane depolarization and rise in cytosolic sodium concentration stimulate the Na + –K + ATPase, which, in turn, increases the net flux of sodium from luminal to the serosal side. These events modify the electrical variables of the intestinal tissues and increase Isc (Wright et al, 1994; Garcia‐Amado et al, 2005).…”