Intestinal digestibility of rumen undegraded protein of formaldehyde-treated feedstuffs measured by mobile bag and in vitro technique

Antoniewicz, A.; Vuuren, A.M. van; Koelen, C.J. van der; Kosmala, I.

doi:10.1016/0377-8401(92)90035-5

Cited by 38 publications

(19 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Considering that the different individual proteins differ in amino acid compo sition and degradation rate within a feed, it is not surprising that in situ investiga tions of individual amino acid degradation have revealed differences in the ex tent of ruminal degradation between individual amino acids in numerous studies (Crooker et al, 1986;Susmel et al, 1989;Antoniewicz et al, 1992;Boila and Ingalls, 1992;Skorko-Sajko et al, 1994;Dakowski et al, 1996;Skiba et al, 1996;Weisbjerg et al, 1996), but in other investigations notable differences have not been observed (Varvikko et al, 1983;Weakley et al, 1983;van Stralen et al, 1997;Piepenbrink and Schingoethe, 1998). Rates of deamination of free amino acids in the rumen have been investigated by Chalupa (1976), who found that lysine, phenylalanine, leucine and isoleucine were broken down at 0.2 to 0.3 mmol h 1 , while arginine and threonine were more labile (0.5-0.9 mmol h 1 ) , methionine and valine were more stabile and deaminated at rates between 0.1 to 0.14 mmol h 1 .…”

Section: Degradation In the Rumen Of Amino Acids In Feed Proteinmentioning

confidence: 99%

Supply of the dairy cow with amino acids from dietary protein

Hvelplund¹,

Misciattelli²,

Weisbjerg³

2001

J. Anim. Feed Sci.

View full text Add to dashboard Cite

Microbial protein produced in the rumen is not sufficient to meet the requirement for amino acids of the high producing dairy cow. The microbial protein thus need to be supplemented with a certain amount of undegraded protein which match the microbial protein both quantitatively and qualitative ly to enable an efficient utilization for milk production. This paper summarizes findings with respect to amino acid composition and digestibility of microbial amino acids. The main emphasis is however on recent findings with respect to degradability and digestibility of individual amino acids in feed proteins. The results seem to indicate that degradability and digestibility estimates obtained for nitro gen may not always apply to individual amino acids. It is concluded that more research is needed to identify the ideal profile of the amino acids absorbed from the intestine of lactating cows. Results of such efforts are needed to compose an ideal protein supplement eventually by using protected amino acids to balance the microbial protein to meet the amino acid requirement of dairy cattle with a minimum of rumen undegraded protein.

show abstract

Section: Degradation In the Rumen Of Amino Acids In Feed Proteinmentioning

confidence: 99%

Supply of the dairy cow with amino acids from dietary protein

Hvelplund¹,

Misciattelli²,

Weisbjerg³

2001

J. Anim. Feed Sci.

View full text Add to dashboard Cite

show abstract

“…Extent of reduction in ruminal degradability and degradation rate of CP depends on the level and method of treatment with formaldehyde (Setala and Syrjala-Qvist, 1984). Antoniewicz et al (1992b) demonstrated that RSM was more susceptible than other feeds (lupins, peas, field beans) to reduced intestinal CP digestibility caused by irreversible protection of CP through treatment with formaldehyde. In the present study, the low level of formaldehyde (1.1 g/kg DM, which is equivalent to 3.1 g/kg CP), applied in gaseous form under pressure, dramatically reduced effective degradability of CP and, to a lesser extent, DM.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of three rape seed commodities in the rumen of steers 1. Degradation of dry matter and crude protein and disappearance of amino acids <i>in situ</i>

Südekum¹,

Andree²

1997

J. Anim. Feed Sci.

View full text Add to dashboard Cite

Ruminal degradation characteristics of rape seed and of untreated and formaldehyde-treated rapeseed meals containing less than 8 /¿mol of total glucosinolates per gram of dry matter were investigated. Feeds were incubated in polyester bags in the rumen of three fistulated steers for 0,2,4, 8, 16, 24 and 48 h. The rate and extent of degradation of dry matter and crude protein and disappearance of amino acids from polyester bags were determined. The crude protein was more degradable than the dry matter of rape seed and untreated rapeseed meal, whereas the opposite was observed for the formaldehyde-treated rapeseed meal. The treatment of the rapeseed meal with formaldehyde greatly reduced the rate and extent of dry matter and crude protein degradation compared with the untreated meal. Effective degradabilities of dry matter and crude protein declined in the following order: rape seed > untreated rapeseed meal > formaldehyde-treated rapeseed meal. The effective degradabilities of crude protein at assumed ruminal passage rates of 2, 5 and 8%/h ranged from 77 to 62% for the rape seed, 76 to 51% for the untreated meal and 39 to 16% for the formaldehyde-treated meal. With increasing exposure time to the rumen, the amino acid profile of undegraded residues of rape seed and of untreated rapeseed meal changed. As a result, large differences among amino acids were observed for percentages of disappearance from the bags. Treatment of rapeseed meal with formaldehyde was effective in preserving the amino acid composition of the undegraded feed protein.

show abstract

“…Obtaining estimates of protein digestion in the small intestine are expensive and labour intensive, and require the use of intestinally surgically prepared animals. Various in vitro laboratory techniques have been used to predict intestinal digestion of proteins in ruminant feed ingredients, including pepsin-HCl with the MNB method (Antoniewicz et al, 1992;Van Straalen et al, 1993) and the three-step technique (Calsamiglia & Stern, 1995).…”

Section: Introductionmentioning

confidence: 99%

Intestinal digestibility of enriched-protein fodders measured by mobile bag incubated with or without pepsin-HCl and three-step techniques

Paengkoum¹,

Traiyakun²,

Paengkoum³

2014

SA J. An. Sci.

View full text Add to dashboard Cite

Ruminal, intestinal and total tract digestibility of dry matter (DM) and crude protein (CP) of leucaena (Leucaena leucocephala), Madras thorn (Pithecellobium dulce) and moringa (Moringa oleifera) fodders were measured in this study, using nylon bag and mobile bag techniques. Three cattle were fitted with permanent rumen and duodenal cannulae. Intestinal digestibility was measured using the mobile nylon bag (MNB) technique with or without incubation in a pepsin-HCl solution, and a three-step in vitro technique. The rate of ruminal disappearances of DM and CP, and the potential degradation of CP from nylon bags of both Madras thorn and moringa fodders were significantly higher than that for leucaena fodder. Potential degradation (A+B) values of CP were 45.6%, 54.2% and 52.8% for leucaena, Madras thorn and moringa fodders, respectively. Average DM and CP digestibility in the intestine and total tract for both Madras thorn and moringa fodders were significantly higher than for leucaena fodder. Average digestibility of DM and CP in the intestine and total tract measured using MNB without pepsin-HCl solution was significantly lower than with pepsin-HCl and with the three-step methods. These data suggest that the results of in vivo and in vitro methods for estimating intestinal digestibility are similar, though in all methods the incubation in a pepsin-HCl solution is necessary. ________________________________________________________________________________

show abstract

Intestinal digestibility of rumen undegraded protein of formaldehyde-treated feedstuffs measured by mobile bag and in vitro technique

Cited by 38 publications

References 17 publications

Supply of the dairy cow with amino acids from dietary protein

Supply of the dairy cow with amino acids from dietary protein

Evaluation of three rape seed commodities in the rumen of steers 1. Degradation of dry matter and crude protein and disappearance of amino acids <i>in situ</i>

Intestinal digestibility of enriched-protein fodders measured by mobile bag incubated with or without pepsin-HCl and three-step techniques

Contact Info

Product

Resources

About