Intestinal Epithelial Cells Modulate Antigen-Presenting Cell Responses to Bacterial DNA

Campeau, Jody; Salim, Saad Y.; Albert, Eric J.; Hotte, Naomi; Madsen, Karen

doi:10.1128/iai.00288-12

Cited by 21 publications

(19 citation statements)

References 30 publications

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“…Data presented in this study indicate that the genomic DNA from B. breve M-16V enhances IFN-γ and IL-10 responses in an IEC-dependent manner. Recently, it was described that oral administration of DNA from B. breve enhances IL-10 and TGF-β production in the colon, indicating an anti-inflammatory role of B. breve DNA [17]. We elaborate on these data by showing that the anti-inflammatory role of B. breve M-16V is similar to the effects exerted by a synthetic CpG DNA.…”

Section: Discussionmentioning

confidence: 95%

“…However, the synthetic CpG DNA but not B. breve M-16V DNA was capable of suppressing IL-13 as well. This may relate to intrinsic immunomodulatory capacities of synthetic CpG DNA, however we cannot exclude the possibility that the purity of the bacterial DNA was suboptimal [17]. Although the combination of synthetic CpG DNA in the presence of scGOS/lcFOS did not consistently further decrease the T h 2 response, the overall balance of the effector response is in favor of a T h 1- and T reg -polarized response.…”

Section: Discussionmentioning

confidence: 99%

“…Follow-up in vitro studies using UV-killed whole bacteria showed that in contrast to Lactobacillus GG, B. breve M-16V did not modulate the effector immune response in IEC/PBMC cocultures [15]. Recently, it has been reported that both DNA from commensals as well as probiotic bacterial strains contain immunosuppressive CpG motifs, thereby facilitating T reg conversion and anti-inflammatory cytokine production [16,17]. Hence, DNA derived from B. breve M-16V, but not membrane components, may play a role in modulating effector immune responses through activation of TLR9 on IEC.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Intestinal Epithelium-Derived Galectin-9 Is Involved in the Immunomodulating Effects of Nondigestible Oligosaccharides

Kivit¹,

Kraneveld²,

Knippels³

et al. 2013

J Innate Immun

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Dietary intervention using nondigestible oligosaccharides, short-chain galacto-oligosaccharides (scGOS)/long-chain fructo-oligosaccharides (lcFOS), in combination with Bifidobacterium breve M-16V prevents allergic disease involving galectin-9. In addition, apical TLR9 signaling contributes to intestinal homeostasis. We studied the contribution of galectin-9 secreted by intestinal epithelial cells (IEC; HT-29 and T84) in T_h1 and regulatory T-cell (T_reg) polarization in vitro. IEC were grown in transwell filters, cocultured with CD3/CD28-activated human peripheral blood mononuclear cells (PBMC) and apically exposed to genomic DNA derived from B. breve M-16V or synthetic TLR9 ligand in the absence or presence of scGOS/lcFOS. Cytokine production and T-cell phenotype were determined and galectin expression by IEC was assessed. Galectin-9 was neutralized using lactose or a TIM-3-Fc fusion protein. IEC exposed to DNA from B. breve M-16V or TLR9 ligand in the presence of scGOS/lcFOS enhanced IFN-γ secretion by PBMC and increased the percentage of T_h1 and T_reg cells. Expression and secretion of galectin-9 by IEC was increased and neutralization of galectin-9 prevented the induction of IFN-γ secretion and also suppressed the production of IL-10 by PBMC. Furthermore, we show that galectin-9 induces T_reg and T_h1 polarization through interaction with antigen-presenting cells. Our findings show that galectin-9 secreted by IEC apically exposed to TLR9 ligand in the presence of scGOS/lcFOS is involved in T_h1 and T_reg polarization and may be a promising target to prevent or treat allergic disease.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intestinal Epithelium-Derived Galectin-9 Is Involved in the Immunomodulating Effects of Nondigestible Oligosaccharides

Kivit¹,

Kraneveld²,

Knippels³

et al. 2013

J Innate Immun

View full text Add to dashboard Cite

show abstract

“…For instance, whereas the direct incubation of DCs with DNA isolated from Bifidobacterium breve or Salmonella Dublin results in very similar inflammatory responses, delivery of bacterial DNA across the epithelium can result in strong reduction of inflammatory cytokine production by DCs but only if the DNA comes from Bi. breve (Campeau et al, 2012). This is associated with a more tolerogenic response in vivo (TGF-β and IL-10 production in colonic tissues) when Bi.…”

Section: Delivery Of Microbial Signalsmentioning

confidence: 91%

Microbial Sensing and Regulation of Mucosal Immune Responses by Intestinal Epithelial Cells

Rescigno

2015

Mucosal Immunology

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“…LPS activated CD14 + monocytes can also stimulate T cell activation and even Th17 differentiation within inflamed intestinal tissues (Bogunovic, Ginhoux et al 2009, Shaw, Kamada et al 2012. Intestinal epithelial cells also possess the ability to differentiate between pathogenic and commensal bacterial antigens and tailor cytokine secretion based on environmental cues (Campeau, Salim et al 2012). …”

Section: Effect Of Egcg On Transcription Factors Important To the Genmentioning

confidence: 99%

Impact of epigallocatechin-3-gallate (EGCG), a broad-spectrum anti-inflammatory, in controlling intestinal factors contributing to inflammatory bowel disease.

Dryden¹

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Intestinal Epithelial Cells Modulate Antigen-Presenting Cell Responses to Bacterial DNA

Cited by 21 publications

References 30 publications

Intestinal Epithelium-Derived Galectin-9 Is Involved in the Immunomodulating Effects of Nondigestible Oligosaccharides

Intestinal Epithelium-Derived Galectin-9 Is Involved in the Immunomodulating Effects of Nondigestible Oligosaccharides

Microbial Sensing and Regulation of Mucosal Immune Responses by Intestinal Epithelial Cells

Impact of epigallocatechin-3-gallate (EGCG), a broad-spectrum anti-inflammatory, in controlling intestinal factors contributing to inflammatory bowel disease.

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