Intestinal Epithelial Stem Cells

Gonzalez, Liara M.

doi:10.1002/9781119063254.ch2

Cited by 1 publication

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ontogenetic development of the mammalian intestine is a topologically and temporally highly organized process. Intestinal stem cells (ISCs) constantly produce enterocytes and endocrine cells to maintain homeostasis in the intestinal epithelium [16]. One of the crucial results of this process is the formation of a specialized intestinal epithelium that exerts digestive and absorptive functions, as well as certain endocrine and immunological roles [16].…”

Section: Introductionmentioning

confidence: 99%

“…Intestinal stem cells (ISCs) constantly produce enterocytes and endocrine cells to maintain homeostasis in the intestinal epithelium [16]. One of the crucial results of this process is the formation of a specialized intestinal epithelium that exerts digestive and absorptive functions, as well as certain endocrine and immunological roles [16]. The survival of newborns and the maintenance of homeostasis require the regulation of the intestinal epithelium operating as a crucial unit [17].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Plant MIR167e-5p Inhibits Enterocyte Proliferation by Targeting β-Catenin

Chen

et al. 2019

Cells

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are important negative regulators of genes involved in physiological and pathological processes in plants and animals. It is worth exploring whether plant miRNAs play a cross-kingdom regulatory role in animals. Herein, we found that plant MIR167e-5p regulates the proliferation of enterocytes in vitro. A porcine jejunum epithelial cell line (IPEC-J2) and a human colon carcinoma cell line (Caco-2) were treated with 0, 10, 20, and 40 pmol of synthetic 2′-O-methylated plant MIR167e-5p, followed by a treatment with 20 pmol of MIR167e-5p for 0, 24, 48, and 72 h. The cells were counted, and IPEC-J2 cell viability was determined by the MTT and EdU assays at different time points. The results showed that MIR167e-5p significantly inhibited the proliferation of enterocytes in a dose- and time-dependent manner. Bioinformatics prediction and a luciferase reporter assay indicated that MIR167e-5p targets β-catenin. In IPEC-J2 and Caco-2 cells, MIR167e-5p suppressed proliferation by downregulating β-catenin mRNA and protein levels. MIR167e-5p relieved this inhibition. Similar results were achieved for the β-catenin downstream target gene c-Myc and the proliferation-associated gene PCNA. This research demonstrates that plant MIR167e-5p can inhibit enterocyte proliferation by targeting the β-catenin pathway. More importantly, plant miRNAs may be a new class of bioactive molecules for epigenetic regulation in humans and animals.

show abstract

Section: Introductionmentioning

confidence: 99%