Intestinal Goblet Cell Differentiation in &lt;i&gt;Nippostrongylus&lt;/i&gt;-Infected Rats after Transfer of Fractionated Thoracic Duct Lymphocytes

Miller, H. R. P.; Nawa, Yukifumi; Parish, Christopher R.

doi:10.1159/000232271

Cited by 52 publications

(27 citation statements)

References 8 publications

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“…This might reflect development of mast cells in lymph nodes, draining regions injected with antigen [35], as a consequence of T-cell stimulation by antigen, either present after the immunizations or added to the cul tures. In support of this view are results showing that development and driving of mast ceils is mediated by T-cell-produced lymphokines [13,19] and that delay ed hypersensitivity contributes to mast cell prolifera tion [2], It is tempting to speculate that such factors al so are responsible for the mucous cell differentiation seen in the epithelium connected to the accumulated lymphoid cells in the lungs, as was indicated recently in mouse lungs [2] and in rat gut [27]. However, mu cous cell differentiation is influenced by the age of the rats [29], making an evaluation of the importance of rnunity to the aerosolized antigen difficult to inter pret, although in the present study age-matched con trols were used.…”

Section: Discussionmentioning

confidence: 76%

“…Previous reports have indicated that these cells can be driven by factors of cell-mediated immunity induced locally [2,13,19,27]. However, unspecific irritation may also induce in creased numbers of mast cells and mucous cells in the lungs [25,36].…”

Section: Discussionmentioning

confidence: 99%

“…Such responses may be localized preferentially in the lym phoid tissue surrounding the bronchioli [10] and the hilar lymph nodes [14]. Proliferation and differentia tion of mast cells [2,13,19] and goblet cells [2,27] have been indicated to be associated with local cellmediated immune responses both in the lung and in the gut. The local presence of immune factors and reactive cells seems to be a prerequisite for allergic reactions like asthma, although 'factors of hyperreac tivity' may also play a role.…”

Section: Introductionmentioning

confidence: 99%

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Immune Responses in Rats Sensitized with Aerosolized Antigen

Ahlstedt¹,

Smedegård²,

Nygren

et al. 1983

Int Arch Allergy Immunol

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Rats of BN × Wi/Fu strain were immunized by aerosol exposure to 0.001–1% of ovalbumin (OA) without using any adjuvant for 2- or 1-week periods with 4-week intervals. The immunization, particularly with the highest dose, induced antibody formation of IgE, measured with RAST, and of IgG and IgA, measured with ELISA. Regional lymph node cells from immunized animals showed spontaneous proliferation which was suppressed by syngeneic spleen cells. The cell mixture could be stimulated by addition of specific antigen or mitogen, while cultures of peripheral lymph node cells showed a less consistent increase in lymphoblast formation. Histological examination of the lungs of immunized animals revealed a rapid onset of inflammation with accumulations of mononuclear cells and also increased numbers of mast cells. There was some dose-response relationship between antigen concentration and cell accumulations. The immunization also seemed to induce differentiation of epithelial cells to mucous cells. Intravenous provocation of animals immunized with 0.01–1% OA aerosol resulted in clinical bronchial reactivity assessed as increased transpulmonary pressure. This airway response was attenuated by pretreatment with indomethacin. Animals immunized with 1% OA did, however, not respond to challenge. These animals showed a decreased response to serotonin compared with nonimmunized controls. In summary, the described animal model seems to provide a tool for studies of the asthmatic reaction.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Immune Responses in Rats Sensitized with Aerosolized Antigen

Ahlstedt¹,

Smedegård²,

Nygren

et al. 1983

Int Arch Allergy Immunol

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“…Recent studies have shown that local immune re sponses including DH in the lung [2] as well as cellmediated immunity in the gut [9,10] and noxious stim ulation of mucosal membranes [8,II,12] are nor mally accompanied by the appearance of increased numbers of mononuclear cells, mast cells and mucusproducing cells. This may partly be due to the produc tion of T-cell-derived lymphokines [7].…”

Section: Discussionmentioning

confidence: 99%

“…Local cell-mediated immune re sponses may induce proliferation and differentiation of mast cells and mucus-producing cells, both in the lung and in the gut [2,9,10]. In addition, mast cells and mucus-producing cells accumulate in response to irritating agents (8,11,12].…”

Section: Introductionmentioning

confidence: 99%

Immunological Suppression of Delayed Hypersensitivity Responses in Mouse Lungs as Reflected by Numbers of Mononuclear Cells, Mast Cells and Mucus-Producing Cells

Enander¹,

Nygren

Ahlstedt

1988

Int Arch Allergy Immunol

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The suppression of delayed hypersensitivity (DH) in the lung as reflected by the appearance of mononuclear cells, mast cells and mucus-producing cells was studied in Balb/c mice. Immunosuppression was induced by intravenous and peroral administration of picrylsulfonic acid (PSA) in mice subsequently sensitized with picrylchloride (PiCl). The animals exhibited a decreased DH reactivity as assessed by ear thickness increase compared with mice sensitized but not exposed to PSA pretreatment. In mice exposed to PSA intravenously (suppressed) and sensitized with PiCl there was a decrease in the number of mononuclear cells in the lung after challenge, compared to mice sensitized and challenged only. Similarly, the mast cell and mucus-producing cell numbers were slightly lower in animals immunosuppressed intravenously with PSA compared with sensitized mice. Such a decrease in the numbers of mononuclear cells, mast cells and mucus-producing cells in the lung was not seen in animals treated with PSA perorally, although these animals exhibited suppressed DH reaction in the ears. The present results indicate that induction of mononuclear cells and to some extent mast cells and mucus-producing cells in the lung relates to the DH reaction and its regulation.

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Effects of guanethidine‐induced sympathectomy on morphology of the pre‐ and postclosure lleum of the neonatal rat

McKenzie

Klein

1982

Am. J. Anat.

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The disappearance of the characteristic supranuclear vacuole and extensive apical canalicular system from enterocytes of the ileal villi occurs during the third postnatal week in rats. This phenomenon is associated with loss of permeability of these cells to macromolecules and is therefore termed closure. The present study was designed to analyze the influence of neonatal guanethidine (GTN)-induced sympathectomy on the morphology of the pre- and postclosure ileum of the rat. Light and electron microscopy of control and GTN-sympathectomized rats demonstrated the retention of immature, vacuolated cells on ileal villi as late as 23 days postnatally in GTN-treated rats. Villi from control rats contained only adultlike nonpermeable cells. Electron microscopy further demonstrated no structural differences in the apical canalicular system or storage vacuoles of the delayed cells in GTN rats when compared to the ileal epithelium from preclosure time periods (7 and 15 days) in both GTN-sympathectomized and control rats. Goblet cells were counted on Periodic-Acid-Schiff-stained sections of ileum from 7, 15, and 23-day GTN and control rats. The percentage of goblet cells in the total epithelial cell population of the villus was significantly higher in control versus GTN rats at all time periods. The percentage of goblet cells increased in both groups from day 7 to 15. However, closure in the control group (approximately day 18) was coincident with a steep increase in the percentage of ileal goblet cells which was not evident in the goblet-cell population of the GTN villus. This pattern of change in control versus GTN goblet-cell production was correlated with a similar pattern of variation in the number of crypt cell mitoses between the two groups over the same time period.

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Intestinal Goblet Cell Differentiation in <i>Nippostrongylus</i>-Infected Rats after Transfer of Fractionated Thoracic Duct Lymphocytes

Cited by 52 publications

References 8 publications

Immune Responses in Rats Sensitized with Aerosolized Antigen

Immune Responses in Rats Sensitized with Aerosolized Antigen

Immunological Suppression of Delayed Hypersensitivity Responses in Mouse Lungs as Reflected by Numbers of Mononuclear Cells, Mast Cells and Mucus-Producing Cells

Effects of guanethidine‐induced sympathectomy on morphology of the pre‐ and postclosure lleum of the neonatal rat

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