Intestinal Helminth Infections in Ghanaian Children from the Ashanti Region between 2007 and 2008—A Retrospective Cross-Sectional Real-Time PCR-Based Assessment

Background Intestinal parasitic infections (IPIs) are serious global public health issues, especially in low and middle-income countries. These parasites can cause high morbidity and mortality, especially in immunocompromised individuals, and can easily be transmitted by consumption of contaminated food or water or by penetration of skin and mucous membranes. Methods We retrospectively analyzed all archived data from stool examination reports at the Parasitology Unit of Precise Health Diagnostic Services, Berekum for the prevalence and trends of parasitic infections spanning a period of 9 years (2013–2021). The data was retrieved and exported to IBM SPSS v.26 for statistical analysis. Descriptive data was summarized as proportions and presented in tables and charts. Pearson Chi-Square test (χ 2 ) or Fisher’s exact test was used to test for a statistical association between demographic factors and intestinal parasitic infections. P value was significant at < 0.05. Results A total of 9,217 records of stool examinations were retrieved. Almost half (48.45%) of the data were from patients aged between 20 and 29 years. An overall prevalence of intestinal parasitic infections of 21.20% was observed in this study. Patients aged 70 to 79 had the highest prevalence of the IPIs (44.44%). The parasites recovered in this study were intestinal flagellates, Entamoeba coli, Hookworm, Entamoeba histolytica/dispar, Ascaris lumbricoides, Enterobius vermicularis, Strongyloides stercoralis and Hymenolepis nana . The intestinal flagellates had the highest prevalence (98.31%) compared to the other intestinal parasites recovered. Intestinal parasitic infections had the highest prevalence in the year 2020 (28.56%) and lowest in 2018 (11.78%). Conclusion An overall prevalence of 21.20% of intestinal parasitic infection was found. The majority of the parasites reported were intestinal flagellates (98.31%). The trend analysis revealed that the overall prevalence fluctuated across the entire period from 2013 to 2021.

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Clustering of Gastrointestinal Microorganisms in Human Stool Samples from Ghana

Backhaus,

Kann,

Hahn

et al. 2024

Pathogens

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The study was conducted to identify cluster patterns of enteric microorganisms with potential etiological relevance for infectious gastroenteritis in stool samples of individuals from Ghana, which is a known high-endemicity setting for infectious gastroenteritis. These patterns were compared to previous observations with specimens from Colombian indigenous people in order to assess potentially stable clustering for temporally and spatially distinct populations from high-endemicity regions. By doing so, the study aimed to identify stable clusters as markers of microbial interaction with potential importance for etiological relevance assignment in cases of multiple enteric pathogen detections. Stool samples from 1569 Ghanaian individuals (875 from HIV patients, 30 from HIV-negative control adult patients, and 644 from children < 2 years of age) were assessed for enteric microorganisms by applying real-time PCR. As a result, nucleic acids of bacterial microorganisms were most frequently detected, followed by protozoa, microsporidia, and helminths. Interestingly, the cluster assessment confirmed interaction patterns known from the previous analysis with Colombian indigenous people, demonstrating a high likelihood of Blastocystis hominis for clustering with other microorganisms and a prominent, potentially mediating role of Dientamoeba fragilis for microbial interactions within the clusters. In conclusion, the assessment confirmed conserved clustering of enteric microorganisms with potential etiological relevance for human infectious gastroenteritis over geographically distinct high-endemicity settings. Furthermore, the composition of abundant microorganisms is more important than regional factors for the determination of the interplay of enteric microorganisms in the human gut. Thereby, some microbial pathogens and commensals seem more susceptible to a changing microbial composition in the human gut than others.

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Intestinal Helminth Infections in Ghanaian Children from the Ashanti Region between 2007 and 2008—A Retrospective Cross-Sectional Real-Time PCR-Based Assessment

Cited by 4 publications

References 60 publications

Prevalence and associated risk factors of soil-transmitted helminth infections among schoolchildren in Mekan Eyesus town, northwestern Ethiopia

Prevalence and associated risk factors of soil-transmitted helminth infections among schoolchildren in Mekan Eyesus town, northwestern Ethiopia

The burden and trend of intestinal parasitosis among women at Berekum, Ghana: a 9-year retrospective study

Clustering of Gastrointestinal Microorganisms in Human Stool Samples from Ghana

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