Intestinal histopathology and immune responses following Escherichia coli lipopolysaccharide challenge in Nile tilapia fed enriched black soldier fly larval (BSF) meal supplemented with chitinase

Agbohessou, Pamphile S.; Mandiki, Syaghalirwa N.M.; Biyong, Serge R. Mbondo; Cornet, Valérie; Nguyen, Thi Mai; Lambert, Jérôme; Jauniaux, Thierry; Lalèyé, Philippe; Kestemont, Patrick

doi:10.1016/j.fsi.2022.08.050

Cited by 11 publications

(12 citation statements)

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“…These bacteria are known for the production of bactericidal compounds and lactic acid which can prevent the colonisation of pathogens on the surface of the gut ( 53 ) and even prevent or repair gut damage caused by anti-nutritional factors contained in vegetable-based ingredients like soyabean meal in fish diet ( 54 ) . The presence of Lactobacillus in fish fed the FOD5 diet could also explain the beneficial effects of this diet on digestibility, growth, intestinal health and better innate immune defence of these fish after being exposed to E. coli lipopolysaccharide ( 30 ) . Moreover, many species of bacteria belonging to the Bacillaceae family can produce chitinase ( 55 ) .…”

Section: Discussionmentioning

confidence: 99%

“…5 (g)). The presence of a greater number of distinct bacterial genera of the phylum Firmicutes in these fish could explain the better intestinal health of fish fed FOD5 compared with those fed VGD0 or VGD5 ( 30 ) . It is thought that species-rich communities may provide additional metabolic capabilities to the host ( 56 ) and compete with pathogens for nutrients and colonisation ( 57 ) .…”

Section: Discussionmentioning

confidence: 99%

“…Analyses of gene expression by quantitative real-time PCR (qPCR) were performed as described previously ( 30 ) . The total RNA of the middle intestine was isolated individually from three fish per tank (nine fish per treatment) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

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Effect of fatty acid-enriched black soldier fly larvae meal combined with chitinase on the metabolic processes of Nile tilapia

Agbohessou,

Mandiki,

Mes

et al. 2024

Br J Nutr

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The aim of this study is to determine to what extent the addition of chitinase to black soldier fly (BSF) larval meal enriched or not with long-chain polyunsaturated fatty acids (LC-PUFAs) could improve growth, protein digestion processes and gut microbial composition in Nile tilapia. Two different types of BSF meal were produced, in which larvae were reared on substrates formulated with vegetable ingredients (VGS) or marine fish offal (FOS). The BSF raised on VGS was enriched in α-linolenic acid-ALA while that raised on FOS was enriched in ALA + eicosapentaenoic acid-EPA +docosahexaenoic-acid-DHA. Six BSF-based diets, enriched or not with chitinase, were formulated and compared with a control diet based on fishmeal and fish oil (FMFO). Two doses (D) of chitinase from Aspergillus niger (2 g and 5 g/kg feed) were added to the BSF larval diets (VGD0 and FOD0) to obtain four additional diets: VGD2, VGD5, FOD2 and FOD5. After 53 days of feeding, results showed that the BSF/FOS based diets induced feed utilization, protein efficiency and digestibility, as well as growth comparable to the FMFO control diet, but better than the BSF/VGS based diets. The supplementation of chitinase to BSF/FOS increased in fish intestine the relative abundance of beneficial microbiota such as those of the Bacillaceae family. The results showed that LC-PUFA enriched BSF meal associated with chitinase could be used as an effective alternative to fishmeal in order to improve protein digestion processes, beneficial microbiota and ultimately fish growth rate.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effect of fatty acid-enriched black soldier fly larvae meal combined with chitinase on the metabolic processes of Nile tilapia

Agbohessou,

Mandiki,

Mes

et al. 2024

Br J Nutr

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“…Among the insects, the black soldier fly (BSF) ( Hermetia illucens , Linnaeus 1758, Diptera, Stratiomyidae family) and its larvae, which are native to the tropical, subtropical, and warm temperate areas of Southern United States (three generation cycles per year) have been shown to be a good replacement of FM and FO ( Hoc et al, 2019 ). In this regard, feed incorporated with different combinations of BSF led to the best overall response in terms of growth and health in trout ( Huyben et al, 2019 ; Fawole et al, 2021 ; Bolton et al, 2021 ; Caimi et al, 2021 ; Hoc et al, 2021 ; Hwang et al, 2021 ; Melenchón et al, 2021 ; Randazzo et al, 2021 ; Cho et al, 2022 ; Liu et al, 2022 ; Ratti et al, 2023 ), salmon ( Belghit et al, 2019 ; Li et al, 2019 ; Weththasinghe et al, 2021 ), clownfish ( Vargas-Abúndez et al, 2019 ), zebrafish ( Zarantoniello et al, 2018 ; Zarantoniello et al, 2019 ), seabass ( Wang et al, 2019 ; Moutinho et al, 2021 ), tilapia ( Yildirim-Aksoy et al, 2020b ; Tippayadara et al, 2021 ; Agbohessou et al, 2022 ), catfish ( Xiao et al, 2018 ; Adeoye et al, 2020 ; Yildirim-Aksoy et al, 2020a ; Sudha et al, 2022 ), and shrimp ( Cummins Jr et al, 2017 ; Yildirim-Aksoy et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…In an attempt to determine the effects of FM replacement with defatted BSF in Jian carp, significant upregulation in the relative gene expression of peroxisome proliferator-activated receptor (PPARα) and heat shock proteins (HSP70) was observed ( Li et al, 2017 ; Zhang et al, 2017 ; Mohan et al, 2022 ). Although BSFL meal supplementation in Nile tilapia did not affect the innate immunity and growth ( Agbohessou et al, 2022 ), BSFL-supplemented meals as an alternative to FM in juvenile seabass substantially increased cytokine expression and mucin production in the gut and skin and exhibited no detrimental effects on development ( Hender et al, 2021 ). Furthermore, according to Chaklader et al (2019) , H. illucens larval diet coupled with poultry by-product meal increased the growth of seabass and considerably elevated immune gene expression in the head kidney.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis and immune gene expression of channel catfish (Ictalurus punctatus) fed diets with inclusion of frass from black soldier fly larvae

Sankappa,

Lange,

Yildirim-Aksoy

et al. 2024

Front. Physiol.

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The larval waste, exoskeleton shedding, and leftover feed components of the black soldier fly and its larvae make up the by-product known as frass. In this study, we subjected channel catfish (Ictalurus punctatus) to a 10-week feeding trial to assess how different dietary amounts of frass inclusion would affect both systemic and mucosal tissue gene expression, especially in regard to growth and immune-related genes. Fish were divided in quadruplicate aquaria, and five experimental diets comprising 0, 50, 100, 200, and 300 g of frass per kilogram of feed were fed twice daily. At the end of the trial, liver, head kidney, gill, and intestine samples were collected for gene expression analyses. First, liver and intestine samples from fish fed with a no frass inclusion diet (control), low-frass (50 g/kg) inclusion diet, or a high-frass (300 g/kg) inclusion diet were subjected to Illumina RNA sequencing to determine global differential gene expression among diet groups. Differentially expressed genes (DEGs) included the upregulation of growth-related genes such as glucose-6-phosphatase and myostatin, as well as innate immune receptors and effector molecules such as toll-like receptor 5, apolipoprotein A1, C-type lectin, and lysozyme. Based on the initial screenings of low/high frass using RNA sequencing, a more thorough evaluation of immune gene expression of all tissues sampled, and all levels of frass inclusion, was further conducted. Using targeted quantitative PCR panels for both innate and adaptive immune genes from channel catfish, differential expression of genes was identified, which included innate receptors (TLR1, TLR5, TLR9, and TLR20A), proinflammatory cytokines (IL-1β type a, IL-1β type b, IL-17, IFN-γ, and TNFα), chemokines (CFC3 and CFD), and hepcidin in both systemic (liver and head kidney) and mucosal (gill and intestine) tissues. Overall, frass from black soldier fly larvae inclusion in formulated diets was found to alter global gene expression and activate innate and adaptive immunity in channel catfish, which has the potential to support disease resistance in this species in addition to demonstrated growth benefits.

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The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases

et al. 2023

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Atlantic salmon (Salmo salar) possesses a genome containing 10 genes encoding chitinases, yet their functional roles remain poorly understood. In other fish species, chitinases have been primarily linked to digestion, but also to other functions, as chitinase‐encoding genes are transcribed in a variety of non‐digestive organs. In this study, we investigated the properties of two chitinases belonging to the family 18 glycoside hydrolase (GH18) group, namely Chia.3 and Chia.4, both isolated from the stomach mucosa. Chia.3 and Chia.4, exhibiting 95% sequence identity, proved inseparable using conventional chromatographic methods, necessitating their purification as a chitinase pair. Detailed biochemical analysis revealed sustained chitinolytic activity against β‐chitin for up to 24 hours, spanning a pH range of 2 to 6. Moreover, subsequent in vitro investigations established that this chitinase pair efficiently degrades diverse chitin‐containing substrates into chitobiose (GlcNAc2), highlighting the potential of Atlantic salmon to utilize novel chitin‐containing feed sources. Analysis of the gastric matrix proteome demonstrate that the chitinases are secreted and rank among the most abundant proteins in the gastric matrix. This finding correlates well with the previously observed high transcription of the corresponding chitinase genes in Atlantic salmon stomach tissue. By shedding light on the secreted chitinases in the Atlantic salmon's stomach mucosa and elucidating their functional characteristics, this study enhances our understanding of chitinase biology in this species. Moreover, the observed capacity to effectively degrade chitin‐containing materials implies the potential utilization of alternative feed sources rich in chitin, offering promising prospects for sustainable aquaculture practices.

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Intestinal histopathology and immune responses following Escherichia coli lipopolysaccharide challenge in Nile tilapia fed enriched black soldier fly larval (BSF) meal supplemented with chitinase

Cited by 11 publications

References 58 publications

Effect of fatty acid-enriched black soldier fly larvae meal combined with chitinase on the metabolic processes of Nile tilapia

Effect of fatty acid-enriched black soldier fly larvae meal combined with chitinase on the metabolic processes of Nile tilapia

Transcriptome analysis and immune gene expression of channel catfish (Ictalurus punctatus) fed diets with inclusion of frass from black soldier fly larvae

The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases

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