Intestinal IFN-γ production is associated with protection from clinical signs, but not with elimination of worms, in Echinostoma caproni infected-mice

Cortés, Alba; Sotillo, Javier; Muñoz-Antolí, Carla; Fried, Bernard; Esteban, J. Guillermo; Toledo, Rafael

doi:10.1007/s00436-014-3851-7

Cited by 9 publications

(4 citation statements)

References 34 publications

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“…Our results indicate that partial and complete resistance to E. caproni infections are associated with goblet cell hyperplasia and changes in mucus glycosylation at the time of challenge infection. Although previous studies have suggested that goblet cell hyperplasia has little effect on resistance to E. caproni infections 16 45 46 , our current results are somewhat confusing. Primary infection induced a goblet cell hyperplasia of about 4-fold greater than in negative controls from 4 wppi.…”

Section: Discussioncontrasting

confidence: 66%

Interleukin-25 Induces Resistance Against Intestinal Trematodes

Muñoz-Antolí

Cortés

Santano

et al. 2016

Sci Rep

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Echinostoma caproni is an intestinal trematode that has been extensively used as an experimental model to investigate the factors determining the resistance to intestinal helminths or the development of chronic infections. ICR mice are permissive hosts for E. caproni in which chronic infections are developed, concomitantly with local Th1 responses, elevated levels of local IFN-γ, inflammation and antibody responses. However, mice develop partial resistance to homologous challenge infections after cure of a primary infection, which converts this subject into an adequate model for the study of the mechanisms generating resistance against intestinal helminths. The purpose of the present study was to compare the immune response induced in primary and secondary infections to elucidate the factors determining the different outcome of the infection in each type of infection. The results obtained indicate that susceptibility is determined by the lack of IL-25 expression in response to primary infection. In contrast, infection in an environment with elevated levels of IL-25, as occurs in challenge infection, results in a Th2 phenotype impairing parasite survival. This was confirmed by treatment of naïve mice with exogenous IL-25 and subsequent infection. Changes induced in goblet cell populations and mucin glycosylation could be implicated in resistance to infection.

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Section: Discussioncontrasting

confidence: 66%

Interleukin-25 Induces Resistance Against Intestinal Trematodes

Muñoz-Antolí

Cortés

Santano

et al. 2016

Sci Rep

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“…This cytokine plays an important role in macrophage activation and modulation of the adaptive T helper one (Th1) immune response (Hu & Ivashkiv, 2009). On the other hand, IFN-γ cannot stimulate a T helper two (Th2) response, which would be the most effective modulation towards elimination of the parasite (Cortes et al ., 2014). It is important to mention that the levels of INF detected were lower than the last point of the standard quantification curve for this cytokine (fig.…”

Section: Discussionmentioning

confidence: 99%

Leukogram and cortisol parameters in Swiss mice experimentally infected withAngiostrongylus costaricensis

et al. 2021

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This study describes changes in haematological parameters, cytokine profile, histopathology and cortisol levels in Swiss mice experimentally infected with Angiostrongylus costaricensis. Twenty-eight Swiss mice were divided into two groups (G1 and G2) of 14 animals each. In each group, eight animals were infected orally with ten third-stage larvae of A. costaricensis and six were used as a control group. The mice of groups G1 and G2 were sacrificed 14 and 24 days after infection, respectively. Samples were collected for histopathological and haematological analyses and determination of the cytokine profile and cortisol levels. Granulomatous reaction, eosinophilic infiltrate and vasculitis in the intestinal tract, pancreas, liver and spleen were observed with varying intensity in infected animals. Our results showed that the mice developed normocytic and hypochromic anaemia, and that the histopathological lesions caused by the experimental infection influenced increases in cortisol, neutrophil and monocyte levels. In addition to this, we detected increased interleukin-6 and tumour necrosis factor alpha levels in the infected animals.

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“…E. caproni infection induces strong local Th1-type responses in ICR mice, which are characterized by high levels of IFN-γ [ 14 ]. In this context, Cortés et al [ 28 ] showed that local IFN-γ production in this host strain seems to play a dichotomous role, inhibiting protective Th2 responses and inducing inflammation and, concomitantly, reducing the severity of the disease and protecting ICR mice from infection-induced morbidity and mortality. INF-γ −/− mice, however, exhibit an enhanced intestinal pathology and lethality after E. caproni infection.…”

Section: Discussionmentioning

confidence: 99%

Differential alterations in the small intestine epithelial cell turnover during acute and chronic infection with Echinostoma caproni (Trematoda)

et al. 2015

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BackgroundThe intestinal epithelium plays a multifactorial role in mucosal defense. In this sense, augmented epithelial cell turnover appears as a potential effector mechanism for the rejection of intestinal-dwelling helminths.MethodsA BrdU pulse-chase experiment was conducted to investigate the infection-induced alterations on epithelial cell kinetics in hosts of high (mouse) and low (rat) compatibility with the intestinal trematode Echinostoma caproni.ResultsHigh levels of crypt-cell proliferation and tissue hyperplasia were observed in the ileum of infected mice, coinciding with the establishment of chronic infections. In contrast, the cell migration rate was about two times higher in the ileum of infected rats compared with controls, with no changes in tissue structure, indicating that an accelerated cell turnover is associated with worm expulsion.ConclusionOur results indicate that E. caproni infection induces a rapid renewal of the intestinal epithelium in the low compatible host that may impair the establishment of proper, stable host-parasite interactions, facilitating worm clearance.

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Intestinal IFN-γ production is associated with protection from clinical signs, but not with elimination of worms, in Echinostoma caproni infected-mice

Cited by 9 publications

References 34 publications

Interleukin-25 Induces Resistance Against Intestinal Trematodes

Interleukin-25 Induces Resistance Against Intestinal Trematodes

Leukogram and cortisol parameters in Swiss mice experimentally infected withAngiostrongylus costaricensis

Differential alterations in the small intestine epithelial cell turnover during acute and chronic infection with Echinostoma caproni (Trematoda)

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