Intestinal microorganisms involved in colorectal cancer complicated with dyslipidosis

Abstract

Background: Abnormal lipid metabolism is considered to be one of main promoters of colorectal cancer (CRC), and intestinal microorganisms may be involved in CRC in patients with abnormal lipid metabolism. Objective: To investigate lipid metabolism in CRC patients and explore the role of intestinal microorganisms in CRC complicated with abnormal lipid metabolism. Methods: Overall, 150 CRC patients in Huzhou Central Hospital from January 2016 to September 2017 were recruited in the present study. Basic patient information and clinical serological indicators were investigated and analyzed. Twenty-one stool samples were collected from patients after receiving informed consent. Next-generation sequencing technology was used to sequence bacterial 16S ribosomal RNA. Bioinformatics analysis was used to profile the microbial composition and screen distinctive bacteria in patients with CRC complicated with abnormal lipid metabolism. Results: Apo B and FFA levels were higher in patients with stage I disease than in patients with other stages. HDL, LDL, Apo B and FFA levels were higher in female patients than in male patients. FFA level was higher in rectal cancer patients than in colon cancer patients. These differences were statistically significant (p < 0.05). The proportion of Escherichia/Shigella was increased in CRC patients with hyperlipoidaemia and hypercholesteremia; the abundance of Streptococcus was increased in CRC patients with hyperlipoidaemia; the abundance of Clostridium XIVa was reduced in CRC patients with hyperlipoidaemia and hypercholesteremia; and the abundance of Ruminococcaceae was reduced in CRC patients with hypercholesteremia. Bilophila and Butyricicoccus were closely related to CRC patients without hyperlipoidaemia or hypercholesteremia, and Selenomonas, Clostridium, Bacteroidetes Slackia, Burkholderiales and Veillonellaceae were closely related to CRC patients with hyperlipoidaemia. Some pathways, including secretion system, chaperones and folding catalysts, amino sugar and nucleotide sugar metabolism, arginine and proline metabolism, glycine, serine and threonine metabolism, histidine metabolism, pores and ion channels, nitrogen metabolism and sporulation, may be involved in lipid metabolism abnormality in CRC patients. Conclusions: Many CRC patients have abnormal lipid metabolism, and the intestinal microbiota is altered in these CRC patients. ARTICLE HISTORY

<p>Adequate Lymph Node Assessments and Investigation of Gut Microorganisms and Microbial Metabolites in Colorectal Cancer</p>

“…The specific research methods were as previously published. 22 Supplementary material 1 includes the specific research methods used for gut microorganism detection.…”

“…17,18 Large numbers of studies have shown that CRC patients have characteristic microbes, including Fusobacteria, Streptococcus, and Clostridium in their intestines. [19][20][21][22] Gut microorganisms are involved in the development and progression of CRCs through many pathways such as through the induction of inflammation and biosynthesis of genotoxins. 23,24 Recent studies have suggested that the occurrence of CRCs is not only the function of a single microorganism, but also the result of the disruption of dynamic equilibrium of the gut microecology.…”

Explaining the genetic causality for complex diseasesviadeep association kernel learning

“…The mechanism between one-carbon metabolism and CRC has been studied 27 and several key mutations in this pathway has been related to CRC 28 . Oocyte meiosis was identified to be associated to colonic diseases in previous study based on expression data 29 and amino sugar and nucleotide sugar metabolism may contribute to the lipid metabolism abnormality in CRC 30 .…”

Analysis of prognosis, genome, microbiome, and microbial metabolome in different sites of colorectal cancer

“…The colorectum, as the storage place of faeces, has the largest number and diversity of microorganisms in the human body. These microorganisms are greatly influenced by the acquired diet, living habits, living, and working environment [25]. Micro ecological environment composed of microorganisms and microbial related metabolites is related to the occurrence and development of CRC [26][27][28][29].…”

“…Micro ecological environment composed of microorganisms and microbial related metabolites is related to the occurrence and development of CRC [26][27][28][29]. Many gut microbes, such as Fusobacteria, Streptococcus and Clostridium [25,[30][31][32], and microbial metabolites, such as hydrolytic, reductive enzymes [33], O(6)-methyl guanine [34], short chain fatty acids and secondary bile acids, are involved in the occurrence of CRCs [26,35,36]. Thus, these factors, including prognosis, RNAs, bacteria, fungi, and microbial metabolites, were selected as indicators to analyse the differences at different sites of CRC in the present study.…”