Intestinal Spirochetosis and Giardiasis in a Beagle Pup with Diarrhea

Duhamel, G; Hunsaker, Breck D.; Mathiesen, Michelle R.; Moxley, Rodney A.

doi:10.1177/030098589603300318

Cited by 26 publications

(20 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has previously been suggested that S. pilosicoli may have synergistic relationships with gastrointestinal protozoa such as Giardia duodenalis [40] and Balantidium coli [21]. Perhaps significantly, S. pilosicoli has recently been isolated from the blood of seven severely-ill patients in France and the United States [19].…”

Section: Discussionmentioning

confidence: 99%

The prevalence of Serpulina pilosicoli in humans and domestic animals in the Eastern Highlands of Papua New Guinea

et al. 1997

View full text Add to dashboard Cite

SUMMARYIn a survey of five villages in the Eastern Highlands of Papua New Guinea, Serpulina pilosicoli was isolated from rectal swabs from 113 of 496 individuals (22n8 %). Colonization rates ranged from 22n6-30n1 % in four of the villages but was only 8n6 % in the other village. In comparison colonization was demonstrated in only 5 of 54 indigenous people (9n3 %) and none of 76 nonindigenous people living in an urban environment in the same region. Colonization did not relate to reported occurrence of diarrhoea, age, sex, or length of time resident in a village. A second set of 94 faecal specimens was collected from 1 village 6 weeks after the first set. S. pilosicoli was isolated from 27 of 29 individuals (93n1 %) who were positive on the first sampling and from 7 of 65 individuals (10n8 %) who previously were negative. In this case, isolates were significantly more common in watery stools than in normal stools. The annual incidence of infection in the village was calculated as 93n6 %, with an average duration of infection of 117 days. S. pilosicoli could not be isolated from any village pig (n l 126) despite its confirmed presence in 17 of 50 commercial pigs (34n0 %) sampled at a local piggery. Four of 76 village dogs (5n3 %) and 1 of 2 village ducks were colonized with S. pilosicoli, suggesting the possibility of cross transmission between humans and animals.

show abstract

Section: Discussionmentioning

confidence: 99%

The prevalence of Serpulina pilosicoli in humans and domestic animals in the Eastern Highlands of Papua New Guinea

et al. 1997

View full text Add to dashboard Cite

show abstract

“…It has been suggested that the complement of canine intestinal spirochaetes consists of B. pilosicoli and a novel species that has been provisionally designated 'Brachyspira canis' (Duhamel et al, 1998;Oxberry & Hampson, 2003 intestinal spirochaetes are found in both healthy and diarrhoeic dogs (Pindak et al, 1965;Turek & Meyer, 1978;Meier et al, 1982;Weber & Schramm, 1989;Lee & Hampson, 1994;Duhamel et al, 1995Duhamel et al, , 1996Duhamel et al, , 1998Lee & Hampson, 1996;Fellström et al, 2001;Oxberry & Hampson, 2003), their aetiological role in disease is still uncertain. However, correlations between clinical presentation and presence of each Brachyspira species suggest that B. pilosicoli is pathogenic, whereas 'B.…”

Section: Introductionmentioning

confidence: 99%

Identification of three clusters of canine intestinal spirochaetes by biochemical and 16S rDNA sequence analysis

Johansson¹,

Duhamel²,

Bergsjø³

et al. 2004

Journal of Medical Microbiology

View full text Add to dashboard Cite

It has been suggested that canine intestinal spirochaetes consist of Brachyspira pilosicoli and a group of strains that has been provisionally designated 'Brachyspira canis'. The purpose of the present study was to compare 22 spirochaete isolates that were obtained from intestinal specimens of dogs in Sweden (n ¼ 12), Norway (n ¼ 4), the United States (n ¼ 3), Australia (n ¼ 2) and Germany (n ¼ 1) with type and reference strains, as well as field isolates, of Brachyspira species by five biochemical tests and determination of almost-complete 16S rDNA sequences. In an evolutionary tree derived from 16S rDNA sequences, the canine isolates grouped into three clusters. One cluster included the type strain of porcine B. pilosicoli, whereas a second larger cluster, which was monophyletic, contained a canine strain that was identified previously as 'B. canis'. The third cluster consisted of three canine isolates of Scandinavian origin, which grouped together with the type strain of the species Brachyspira alvinipulli (pathogenic to chicken). These three genotypes, which were identified on the basis of 16S rDNA sequences, corresponded to four phenotypic groups based on biochemical testing. Two biochemical tests, hippurate hydrolysis and AEgalactosidase production, were sufficient for rapid identification of each canine cluster.

show abstract

“…Previously, both Giardia [14] and Balantidium coli [15] have been suggested to have synergistic relationships with intestinal spirochaetes. In a study of homosexual men [16], spirochaete carriage was PFGE was carried out on only 39 of the 40 B. pilosicoli isolates, as isolate WHP15 could not be recovered from storage.…”

mentioning

confidence: 99%

Carriage of intestinal spirochaetes by humans: epidemiological data from Western Australia

Brooke

Clair²,

Mikosza

et al. 2001

Epidemiol. Infect.

View full text Add to dashboard Cite

SUMMARYThe purpose of this study was to investigate carriage of intestinal spirochaetes by selected population groups in Western Australia. Stool specimens from 293 rural patients with gastrointestinal disorders, and from 227 healthy migrants from developing countries were cultured. Spirochaete isolates were identified using PCR, and typed by pulsed field gel electrophoresis (PFGE). Brachyspira aalborgi was not isolated. Brachyspira pilosicoli was recovered from 15 rural patients, all Aboriginal. Prevalence was 9n9 % in 151 Aboriginals and 0 % in 142 non-Aboriginals. Carriage of B. pilosicoli amongst migrants was 10n6 % (24\227). Carriage was significantly increased in Aboriginal children aged 2-5 years (P l 0n0027) and in migrant individuals from the Middle East and Africa (P l 0n0034). Carriage was significantly associated with detection of faecal protozoa in both Aboriginals (P l 0n0021) and migrants (P l 0n012). PFGE results indicated that the B. pilosicoli strains were genetically diverse.

show abstract

Intestinal Spirochetosis and Giardiasis in a Beagle Pup with Diarrhea

Cited by 26 publications

References 8 publications

The prevalence of Serpulina pilosicoli in humans and domestic animals in the Eastern Highlands of Papua New Guinea

The prevalence of Serpulina pilosicoli in humans and domestic animals in the Eastern Highlands of Papua New Guinea

Identification of three clusters of canine intestinal spirochaetes by biochemical and 16S rDNA sequence analysis

Carriage of intestinal spirochaetes by humans: epidemiological data from Western Australia

Contact Info

Product

Resources

About