Intestinal TM7 bacterial phylogenies in active inflammatory bowel disease

Kuehbacher, Tanja; Rehman, Ateequr; Lepage, Patricia; Hellmig, Stephan; Fölsch, Ulrich R.; Schreiber, Stefan; Ott, Stephan

doi:10.1099/jmm.0.47719-0

Cited by 168 publications

(136 citation statements)

References 33 publications

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“…2B). A high abundance of candidate phylum TM7 has been implicated in inflammatory mucosal diseases (33)(34)(35), and a particular TM7 phylotype was shown to be parasitic and a potential immune suppressant (36). An increase in TM7 abundance from 1 to 21% in the oral cavity is associated with periodontal disease (37).…”

Section: Resultsmentioning

confidence: 99%

Human and rat gut microbiome composition is maintained following sleep restriction

Zhang

Bai

Goel

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

117

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Insufficient sleep increasingly characterizes modern society, contributing to a host of serious medical problems. Loss of sleep is associated with metabolic diseases such as obesity and diabetes, cardiovascular disorders, and neurological and cognitive impairments. Shifts in gut microbiome composition have also been associated with the same pathologies; therefore, we hypothesized that sleep restriction may perturb the gut microbiome to contribute to a disease state. In this study, we examined the fecal microbiome by using a cross-species approach in both rat and human studies of sleep restriction. We used DNA from hypervariable regions (V1-V2) of 16S bacteria rRNA to define operational taxonomic units (OTUs) of the microbiome. Although the OTU richness of the microbiome is decreased by sleep restriction in rats, major microbial populations are not altered. Only a single OTU, TM7-3a, was found to increase with sleep restriction of rats. In the human microbiome, we find no overt changes in the richness or composition induced by sleep restriction. Together, these results suggest that the microbiome is largely resistant to changes during sleep restriction.

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Section: Resultsmentioning

confidence: 99%

Human and rat gut microbiome composition is maintained following sleep restriction

Zhang

Bai

Goel

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

117

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“…Little is known about this bacterial phylogeny, however it has been suggested to contribute to inflammatory pathologies within the gastrointestinal tract (32). More importantly, BALB/c-Tlr4 -/-billy mice had elevated levels of -glucuronidase-producing proteobacteria, likely increasing the rate of SN-38 reactivation, and thus worsened gut toxicity.…”

Section: Discussionmentioning

confidence: 99%

Irinotecan-Induced Gastrointestinal Dysfunction and Pain Are Mediated by Common TLR4-Dependent Mechanisms

Wardill

Gibson

Sebille

et al. 2016

Molecular Cancer Therapeutics

123

124

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Strong epidemiological data indicate that chemotherapy-induced gut toxicity and pain occur in parallel, indicating common underlying mechanisms. We have recently outlined evidence suggesting that TLR4 signaling may contribute to both side effects. We therefore aimed to determine if genetic deletion of TLR4 improves chemotherapy-induced gut toxicity and pain. Forty-two female wild-type (WT) and 42 Tlr4 null (−/−) BALB/c mice weighing between 18 and 25 g (10–13 weeks) received a single 270 mg/kg (i.p.) dose of irinotecan hydrochloride or vehicle control and were killed at 6, 24, 48, 72, and 96 hours. Bacterial sequencing was conducted on cecal samples of control animals to determine the gut microbiome profile. Gut toxicity was assessed using validated clinical and histopathologic markers, permeability assays, and inflammatory markers. Chemotherapy-induced pain was assessed using the validated rodent facial grimace criteria, as well as immunologic markers of glial activation in the lumbar spinal cord. TLR4 deletion attenuated irinotecan-induced gut toxicity, with improvements in weight loss (P = 0.0003) and diarrhea (P < 0.0001). Crypt apoptosis was significantly decreased in BALB/c-Tlr4−/−billy mice (P < 0.0001), correlating with lower mucosal injury scores (P < 0.005). Intestinal permeability to FITC-dextran (4 kDa) and LPS translocation was greater in WT mice than in BALB/c-Tlr4−/−billy (P = 0.01 and P < 0.0001, respectively). GFAP staining in the lumbar spinal cord, indicative of astrocytic activation, was increased at 6 and 72 hours in WT mice compared with BALB/c-Tlr4−/−billy mice (P = 0.008, P = 0.01). These data indicate that TLR4 is uniquely positioned to mediate irinotecan-induced gut toxicity and pain, highlighting the possibility of a targetable gut/CNS axis for improved toxicity outcomes. Mol Cancer Ther; 15(6); 1376–86. ©2016 AACR.

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“…Candidate division TM7 bacteria have also been detected at a number of human body sites, including the skin (10), distal esophagus (11), gut (12), and the oral cavity (3,(13)(14)(15)(16)(17)(18). Initially, TM7 was found in relatively low abundance in oral cavity population samples (13), but in a more recent study, a high abundance of TM7 in subgingival plaque seemed to correlate with periodontal disease (3).…”

mentioning

confidence: 99%

Axenic Culture of a Candidate Division TM7 Bacterium from the Human Oral Cavity and Biofilm Interactions with Other Oral Bacteria

Soro

Dutton

Sprague

et al. 2014

Appl Environ Microbiol

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cThe diversity of bacterial species in the human oral cavity is well recognized, but a high proportion of them are presently uncultivable. Candidate division TM7 bacteria are almost always detected in metagenomic studies but have not yet been cultivated. In this paper, we identified candidate division TM7 bacterial phylotypes in mature plaque samples from around orthodontic bonds in subjects undergoing orthodontic treatment. Successive rounds of enrichment in laboratory media led to the isolation of a pure culture of one of these candidate division TM7 phylotypes. The bacteria formed filaments of 20 to 200 m in length within agar plate colonies and in monospecies biofilms on salivary pellicle and exhibited some unusual morphological characteristics by transmission electron microscopy, including a trilaminated cell surface layer and dense cytoplasmic deposits. Proteomic analyses of cell wall protein extracts identified abundant polypeptides predicted from the TM7 partial genomic sequence. Pleiomorphic phenotypes were observed when the candidate division TM7 bacterium was grown in dual-species biofilms with representatives of six different oral bacterial genera. The TM7 bacterium formed long filaments in dual-species biofilm communities with Actinomyces oris or Fusobacterium nucleatum. However, the TM7 isolate grew as short rods or cocci in dual-species biofilms with Porphyromonas gingivalis, Prevotella intermedia, Parvimonas micra, or Streptococcus gordonii, forming notably robust biofilms with the latter two species. The ability to cultivate TM7 axenically should majorly advance understanding of the physiology, genetics, and virulence properties of this novel candidate division oral bacterium.

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Intestinal TM7 bacterial phylogenies in active inflammatory bowel disease

Cited by 168 publications

References 33 publications

Human and rat gut microbiome composition is maintained following sleep restriction

Human and rat gut microbiome composition is maintained following sleep restriction

Irinotecan-Induced Gastrointestinal Dysfunction and Pain Are Mediated by Common TLR4-Dependent Mechanisms

Axenic Culture of a Candidate Division TM7 Bacterium from the Human Oral Cavity and Biofilm Interactions with Other Oral Bacteria

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