Intestinal transepithelial passage of bovine milk protein antigens in vitro

Marcon-Genty, D.; Kheroua, Omar; Tomé, Daniel; Dumontier, A M; Desjeux, Jehan-François

doi:10.1203/00006450-198708000-00059

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have preliminary evidence that HRP is an appropriate marker of cow's milk proteins such a 0-lactoglobulin or a-lactalbumin. Indeed, in rabbit intestine, these two proteins are transported to a similar extent as HRP in our study (23). Milk protein transport should be measured across human intestinal biopsies before conclusions can be drawn, however, we think that HRP transport might well reflect 0-lactoglobulin or a-lactalbumin transport.…”

Section: Discussionmentioning

confidence: 99%

Antigen Absorption by the Jejunal Epithelium of Children with Cow's Milk Allergy

Grasset²,

et al. 1988

View full text Add to dashboard Cite

ABSTRACT. To establish if intestinal permeability to exogenous antigens is involved in cow's milk allergy (CMA) in infants, 33 children 1 to 24 months old (18 controls and 1 5 with CMA) were tested for intestinal permeability to the protein marker horseradish peroxidase (HRP). Jejunal biopsies were performed either during the initial period of diagnosis, at the mean (and SE) age of 3 f 1 months, and/or 1 yr later, at the age of 13 f 2 months, just before and after a milk challenge. A small fragment of the biopsy was studied for histology and the remainder was mounted in an Ussing chamber for simultaneous measurement of mucosal to serosal transport of H R P in its intact and degraded forms and electrical parameters including short-circuit current and conductance. No modification in H R P absorption was noted in control children aged from 2 months to 11 yr, indicating that gut closure probably occurred earlier in life. During the initial period of CMA, transepithelial H R P fluxes were significantly higher, about 8-fold, in children with CMA (intact H R P flux = 48.5 f 15.2, 95% confidence interval, 11.2 to 85.7 versus 5.9 + 1.2, 95% confidence interval 2.9 to 8.3, in control children) In addition, short-circuit current was increased but conductance was unchanged. After several months on a milkfree diet, H R P flux and short-circuit current returned to control values. Just after the milk challenge and independently of -the clinical issue, a slight rise in H R P permeability was observed but it was not significant and remained within control values. These results suggest that increased permeability to proteins is probably not the primary cause of CMA, and that the increase in protein endocytosis noted during the initial period of CMA seems rather to be a secondary effect of an abnormal immunological response leading to mucosal inflammation and impairment of the endocytic process. (Pediatr Res 24:197-202, 1988)

show abstract

Section: Discussionmentioning

confidence: 99%