Intestinal Uptake and Transport of Albumin Nanoparticles: Potential for Oral Delivery

Hashem, Lina; Swedrowska, Magda; Vllasaliu, Driton

doi:10.2217/nnm-2018-0029

Cited by 38 publications

(23 citation statements)

References 23 publications

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“…Additionally, caveolae-mediated endocytosis of 100 nm fluorescent PS-particles was shown to be dependent on the abundance of albumin on the corona surface, supporting the idea that it might be an important driver for cellular uptake of PS-particles [61]. Our additional results with HSA containing media are an extension to studies demonstrating NP transfer by using cell barrier systems in the presence of albumin [58,59].…”

Section: Discussionsupporting

confidence: 71%

“…Both proteins were found on protein coronas of PSparticles before [1]. Albumin was tested to functionalize nanomaterials to enhance cellular uptake, target cells, or study transfer in several models [58][59][60]. Additionally, caveolae-mediated endocytosis of 100 nm fluorescent PS-particles was shown to be dependent on the abundance of albumin on the corona surface, supporting the idea that it might be an important driver for cellular uptake of PS-particles [61].…”

Section: Discussionmentioning

confidence: 74%

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Plasma proteins facilitates placental transfer of polystyrene particles

et al. 2020

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Background Nanoparticles, which are exposed to biological fluids are rapidly interacting with proteins and other biomolecules forming a corona. In addition to dimension, charge and material the distinct protein corona influences the interplay of nanoparticles with tissue barriers. In this study we were focused on the impact of in situ formed human plasma protein corona on the transfer of 80 nm polystyrene nanoparticles (PS-particles) across the human placenta. To study materno-to fetal PS transfer we used the human ex vivo placental perfusion approach, which represents an intact and physiological tissue barrier. To analyze the protein corona of PS particles we performed shotgun proteomics of isolated nanoparticles before and after tissue exposure. Results Human plasma incubated with PS-particles of 80 nm and subsequent formed protein corona enhanced the transfer across the human placenta compared to PS-corona formed by bovine serum albumin and dextran which served as a control. Quantitative and qualitative changes of plasma proteins determined the changes in PS transfer across the barrier. Based on the analysis of the PS-proteome two candidate proteins, namely human albumin and immunoglobulin G were tested if these proteins may account for the enhanced PS-transfer across the placenta. Interestingly, the protein corona formed by human albumin significantly induced the transfer of PS-particles across the tissue compared to the formed IgG-corona. Conclusion In total we demonstrate the PS corona dynamically and significantly evolves upon crossing the human placenta. Thus, the initial composition of PS particles in the maternal circulation is not predictive for their transfer characteristics and performance once beyond the barrier of the placenta. The precise mechanism of these effects remains to be elucidated but highlights the importance of using well designed biological models when testing nanoparticles for biomedical applications.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 74%

Plasma proteins facilitates placental transfer of polystyrene particles

et al. 2020

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“…As part of these strategies, vesicle-or particle-based nanoscale drug carriers have been studied for some time. This includes polymeric nanoparticles [5], liposomes [6,7] and solid-lipid nanoparticles [6,8]. However, these systems tend to suffer from inefficient translocation across the highly effective and selective barrier of the intestinal mucosa, therefore requiring careful formulation.…”

Section: Introductionmentioning

confidence: 99%

Cow Milk and Intestinal Epithelial Cell-Derived Extracellular Vesicles as Systems for Enhancing Oral Drug Delivery

et al. 2020

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Ingestion is the preferred way for drug administration. However, many drugs have poor oral bioavailability, warranting the use of injections. Extracellular vesicles (EVs) from cow milk have shown potential utility in improving oral drug bioavailability. However, EVs produced by intestinal epithelial cells have not been investigated for this application. We compared the capacity of cow milk EVs and intestinal epithelial cell-derived counterparts to enhance oral drug bioavailability. EVs were isolated, fluorescently labelled, and loaded with curcumin (CUR) as a model poorly absorbable drug. These were then characterised before testing in an intestinal model (Caco-2). Epithelial cell-derived EVs showed notably higher cell uptake compared to cow milk EVs. Cell uptake was significantly higher in differentiated compared to undifferentiated cells for both types of EVs. While both milkand cell-derived EVs improved the cell uptake and intestinal permeability of CUR (confirming oral drug bioavailability enhancement potential), epithelial cell EVs demonstrated a superior effect.

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“…88 The Caco-2 model has been employed widely for the study or nanomedicines for oral delivery. 3, 89,90 However, the Caco-2 transwell system accounts for neither the constant uid ow nor the uid shear stress tolerated by epithelial cells in vivo, thereby skewing the predicted bioavailability of chemicals and/or nanomedicines. 91,92 Caco-2 cell monolayers additionally lack a mucus coating, which serves to (i) control the intestinal absorption of matter and (ii) shield the epithelium from harmful intraluminal contents in vivo.…”

Section: Gastrointestinal Models For the Study Of Nanoparticle Behaviourmentioning

confidence: 99%

Nanoparticle modification in biological media: implications for oral nanomedicines

et al. 2019

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Intestinal Uptake and Transport of Albumin Nanoparticles: Potential for Oral Delivery

Abstract: This study highlights for the first time that simply fabricated, nontoxic human serum albumin nanoparticles may find application in oral drug delivery.

Cited by 38 publications

References 23 publications

Plasma proteins facilitates placental transfer of polystyrene particles

Plasma proteins facilitates placental transfer of polystyrene particles

Cow Milk and Intestinal Epithelial Cell-Derived Extracellular Vesicles as Systems for Enhancing Oral Drug Delivery

Nanoparticle modification in biological media: implications for oral nanomedicines

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