Intestinal Yeast Floras of Successive Populations of Drosophila

Shihata, Amal; Mrak, E. M.

doi:10.2307/2405417

Cited by 22 publications

(12 citation statements)

References 11 publications

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“…However, one of the possible explanations of the small differentiation between wine estates B and C in comparison to A is the short distance between B and C, which have juxtaposed vineyard plots. At such a short distance, insects like bees, wasps, and fruit flies, as well as birds, which are known to be vectors for yeasts, could have homogenized these yeast populations (51,54,55). Humans can also influence the yeast population structure and promote dispersal (49).…”

Section: Discussionmentioning

confidence: 99%

Cellar-Associated Saccharomyces cerevisiae Population Structure Revealed High-Level Diversity and Perennial Persistence at Sauternes Wine Estates

Börlin

Venet

Claisse

et al. 2016

Appl Environ Microbiol

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Three wine estates (designated A, B, and C) were sampled in Sauternes, a typical appellation of the Bordeaux wine area producing sweet white wine. From those wine estates, 551 yeast strains were collected between 2012 and 2014, added to 102 older strains from 1992 to 2011 from wine estate C. All the strains were analyzed through 15 microsatellite markers, resulting in 503 unique Saccharomyces cerevisiae genotypes, revealing high genetic diversity and a low presence of commercial yeast starters. Population analysis performed using F st genetic distance or ancestry profiles revealed that the two closest wine estates, B and C, which have juxtaposed vineyard plots and common seasonal staff, share more related isolates with each other than with wine estate A, indicating exchange between estates. The characterization of isolates collected 23 years ago at wine estate C in relation to recent isolates obtained at wine estate B revealed the long-term persistence of isolates. Last, during the 2014 harvest period, a temporal succession of ancestral subpopulations related to the different batches associated with the selective picking of noble rotted grapes was highlighted. IMPORTANCEHigh genetic diversity of S. cerevisiae isolates from spontaneous fermentation on wine estates in the Sauternes appellation of Bordeaux was revealed. Only 7% of all Sauternes strains were considered genetically related to specific commercial strains. The long-term persistence (over 20 years) of S. cerevisiae profiles on a given wine estate is highlighted.

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Section: Discussionmentioning

confidence: 99%

Cellar-Associated Saccharomyces cerevisiae Population Structure Revealed High-Level Diversity and Perennial Persistence at Sauternes Wine Estates

Börlin

Venet

Claisse

et al. 2016

Appl Environ Microbiol

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“…According to Shehata and Mrak (1952), Dobzhansky in 1948 'expressed the opinion that yeasts are important in understanding some of the forces of natural selection to which the natural populations of Drosophila are exposed'. Subsequent studies of the relationships between yeasts and Drosophila have contributed greatly to the understanding of Drosophila ecology (Carson 1971) and have vindicated Dobzhansky's opinion.…”

Section: Introductionmentioning

confidence: 99%

Attraction of Drosophila Buzzatii and D. Aldrichi to Species of Yeasts Isolated From their Natural Environment. I. Laboratory Experiments

Barker¹,

Parker²,

Toll³

et al. 1981

Aust. Jnl. Of Bio. Sci.

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The attractiveness of yeast species isolated from rotting c1adodes of Opuntia inermis for adults of the cactophilic species D. buzzatii and D. aldrichi was determined by giving the flies a multiple choice of yeast species and counting the numbers on each yeast at regular intervals throughout 1 day in each of five experiments. Consistent effects implying behavioural differences between sexes, between immature and mature flies, and between the two Drosophila species were found. For immature flies of each species, there were significant differences in the numbers of flies attracted to each yeast, but preferences were more marked for females than for males. Although there were no significant differences between the two Drosophila species in their yeast preferences, comparison of results for 1-3-day-old flies and mature mated females suggested possible differentiation between the species in yeast utilization by mature females, and possible larval-adult feeding niche separation. Behavioural responses contributing to observed effects, particularly differences between sexes, and between young and mature flies, are clearly complex and their basis remains to be elucidated.

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“…Interest in the microorganisms upon which Drosophila feed in nature dates back about 35 years (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). The researchers who participated in these early studies realized the importance of putting the population genetics ofDrosophila into an ecological context.…”

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confidence: 99%

Larval selectivity for yeast species by Drosophila mojavensis in natural substrates

Fogleman

Starmer

Heed

1981

Proc. Natl. Acad. Sci. U.S.A.

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The yeast flora found in the major substrates of Drosophila mojavensis and in larval guts was studied both qualitatively and quantitatively. Quantitative studies show that, in four of the five substrates tested, the larvae did not contain a random sample of the yeasts present in the substrates. One widely distributed cactus yeast, Pichia cactophila, was typically in greater frequency in the larvae than in the substrates. Another cactus yeast, Candida sonorensis, typically exhibited the opposite relationship. Laboratory tests support larval preference behavior rather than differential digestion as being primarily responsible. Larvae are capable of distinguishing between patches of different yeast species and spend more time in patches of preferred yeasts. D. mojavensis appear to be ecological (host plants) generalists and physiological (yeasts) specialists in contrast to the other cactophilic Drosophila Selective feeding by D. mojavensis larvae in natural substrates may represent optimal foraging behavior.Interest in the microorganisms upon which Drosophila feed in nature dates back about 35 years (1-12). The researchers who participated in these early studies realized the importance of putting the population genetics ofDrosophila into an ecological context. Food sources are certainly major factors of the ecology of any animal, and yeasts are considered to be a major food source for the majority of species of Drosophila in both adult and larval stages (13). The last decade, however, has seen an acceleration ofinterest in this subject, particularly in the yeasts that inhabit the decaying stems of various species of columnar cacti in the Sonoran Desert.The Sonoran Desert of the southwestern United States and northwestern Mexico has provided a unique opportunity to study yeasts and their relationship to Drosophila species because the breeding and feeding sites of cactophilic Drosophila are well known (14,15 (26).The isolation ofyeasts from larval guts and specific substrates was accomplished as follows: Naturally occurring necroses were examined for the presence of Drosophila larvae. If they were present, three or four 1-g samples of the rotting tissue were collected from the area of the rot containing the larvae. Six to eight second-or third-instar larvae were also collected. The larvae were surface sterilized in 70% (vol/vol) The publication costs ofthis article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.

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Intestinal Yeast Floras of Successive Populations of Drosophila

Cited by 22 publications

References 11 publications

Cellar-Associated Saccharomyces cerevisiae Population Structure Revealed High-Level Diversity and Perennial Persistence at Sauternes Wine Estates

Cellar-Associated Saccharomyces cerevisiae Population Structure Revealed High-Level Diversity and Perennial Persistence at Sauternes Wine Estates

Attraction of Drosophila Buzzatii and D. Aldrichi to Species of Yeasts Isolated From their Natural Environment. I. Laboratory Experiments

Larval selectivity for yeast species by Drosophila mojavensis in natural substrates

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