Intestine-derived Clostridium leptum induces murine tolerogenic dendritic cells and regulatory T cells in vitro

Li, Yanan; Huang, Fei; Cheng, Huanji; Li, Shanyu; Liu, Li; Wang, Liying

doi:10.1016/j.humimm.2014.09.017

Cited by 18 publications

(14 citation statements)

References 37 publications

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“…Colonic colonization of Clostridium spp. was related to accumulation of Treg lymphocytes which inhibited the development of inflammatory lesions [67]. Two Clostridium IV species including Clostridium leptum and Eubacterium siraeum were significantly decreased in our CKD rats.…”

Section: Discussionmentioning

confidence: 59%

Microbiome–metabolomics reveals gut microbiota associated with glycine-conjugated metabolites and polyamine metabolism in chronic kidney disease

Feng

Cao

Chen

et al. 2019

Cell. Mol. Life Sci.

165

100

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Dysbiosis of the gut microbiome and related metabolites in chronic kidney disease (CKD) have been intimately associated with the prevalence of cardiovascular diseases. Unfortunately, thus far, there is a paucity of sufficient knowledge of gut microbiome and related metabolites on CKD progression partly due to the severely limited investigations. Using a 5/6 nephrectomized (NX) rat model, we carried out 16S rRNA sequence and untargeted metabolomic analyses to explore the relationship between colon's microbiota and serum metabolites. Marked decline in microbial diversity and richness was accompanied by significant changes in 291 serum metabolites, which were mediated by altered enzymatic activities and dysregulations of lipids, amino acids, bile acids and polyamines metabolisms. Interestingly, CCr was directly associated with some microbial genera and polyamine metabolism. However, SBP was directly related to certain microbial genera and glycine-conjugated metabolites in CKD rats. Administration of poricoic acid A (PAA) and Poria cocos (PC) ameliorated microbial dysbiosis as well as attenuated hypertension and renal fibrosis. In addition, treatments with PAA and PC lowered serum levels of microbial-derived products including glycine-conjugated compounds and polyamine metabolites. Collectively, the present study confirmed the CKD-associated gut microbial dysbiosis and identified a novel dietary and therapeutic strategy to improve the gut microbial dysbiosis and the associated metabolomic disorders and retarded the progression of kidney disease in the rat model of CKD.

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Section: Discussionmentioning

confidence: 59%

Microbiome–metabolomics reveals gut microbiota associated with glycine-conjugated metabolites and polyamine metabolism in chronic kidney disease

Feng

Cao

Chen

et al. 2019

Cell. Mol. Life Sci.

165

100

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“…TGF-β is a cytokine involved in the secretion of interleukin 17 (IL-17) by Th17 and Treg cells which contribute to the inflammatory process in the intestine [ 72 ], and its production is stimulated by the presence of C . leptum [ 6 , 73 ]. Within the TGF-β pathway, our QTL zone contained i) HTRA1 which inhibits signals from TGF-β family members [ 74 ], ii) RAB11FIP2 which is involved in the limitation of inflammatory responses to commensal bacteria through its action of compartmentalization of Toll-Like receptors [ 75 ], iii) ADAM12 which limits the production of IL-17 by Th17 cells [ 72 ], and iv) C10ORF88 which is also involved in the secretion of IL-17 by Th17 cells through its effect on vitamin D metabolism [ 76 ].…”

Section: Resultsmentioning

confidence: 99%

Impact of Selection for Digestive Efficiency on Microbiota Composition in the Chicken

et al. 2015

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ObjectivesFeed efficiency and its digestive component, digestive efficiency, are key factors in the environmental impact and economic output of poultry production. The interaction between the host and intestinal microbiota has a crucial role in the determination of the ability of the bird to digest its food and to the birds’ feed efficiency. We therefore investigated the phenotypic and genetic relationships between birds’ efficiency and the composition of the cecal microbiota in a F2 cross between broiler lines divergently selected for their high or low digestive efficiency.MethodsAnalyses were performed on 144 birds with extreme feed efficiency values at 3 weeks, with feed conversion values of 1.41±0.05 and 2.02±0.04 in the efficient and non-efficient groups, respectively. The total numbers of Lactobacillus, L. salivarius, L. crispatus, C. coccoides, C. leptum and E. coli per gram of cecal content were measured.ResultsThe two groups mainly differed in larger counts of Lactobacillus, L. salivarius and E. coli in less efficient birds. The equilibrium between bacterial groups was also affected, efficient birds showing higher C. leptum, C. coccoides and L. salivarius to E. coli ratios. The heritability of the composition of microbiota was also estimated and L. crispatus, C. leptum, and C. coccoides to E. coli ratios were moderately but significantly heritable (0.16 to 0.24). The coefficient of fecal digestive use of dry matter was genetically and positively correlated with L. crispatus, C. leptum, C. coccoides (0.50 to 0.76) and negatively with E. coli (-0.66). Lipid digestibility was negatively correlated with E. coli (-0.64), and AMEn positively correlated with C. coccoides and with the C. coccoides to Lactobacillus ratio (0.48 to 0.64). We also detected 14 Quantitative Trait Loci (QTL) for microbiota on the host genome, mostly on C. leptum and Lactobacillus. The QTL for C. leptum on GGA6 was close to genome-wide significance. This region mainly includes genes involved in anti-inflammatory responses and in the motility of the gastrointestinal tract.

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“…This resulted in their enhanced susceptibility to E. coli and Klebsiella pneumoniae infection regulated by IL-17-producing innate lymphoid cells. NK cells and monocyte/DC subsets could also be modulated by the microbiota (19,44). In germ-free mice, NK cells develop, albeit with a greater fraction expressing an immature phenotype, and they are poorly primed and activated by microbial ligands or during infection (43).…”

Section: High-stringency Sterile Environments Are Also Deleterious Tomentioning

confidence: 99%

“…In adult mice, transient or permanent alterations in the density and diversity of the GIT microbiota affect the maintenance and function of immune cell populations required for mucosal and systemic immune homeostasis (18)(19)(20)(21). T cell immunity is critical for control and clearance of a viral infection, and antibiotic treatment of adult mice alters antiviral innate and adaptive immunity (22,23).…”

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confidence: 99%

Maternal Antibiotic Treatment Impacts Development of the Neonatal Intestinal Microbiome and Antiviral Immunity

González‐Pérez

Hicks

Tekieli

et al. 2016

The Journal of Immunology

134

112

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Microbial colonization of the infant gastrointestinal tract (GIT) begins at birth, is shaped by the maternal microbiota, and is profoundly altered by antibiotic treatment. Antibiotic treatment of mothers during pregnancy influences colonization of the GIT microbiota of their infants. The role of the GIT microbiota in regulating adaptive immune function against systemic viral infections during infancy remains undefined. We used a mouse model of perinatal antibiotic exposure to examine the effect of GIT microbial dysbiosis on infant CD8+ T cell–mediated antiviral immunity. Maternal antibiotic treatment/treated (MAT) during pregnancy and lactation resulted in profound alterations in the composition of the GIT microbiota in mothers and infants. Streptococcus spp. dominated the GIT microbiota of MAT mothers, whereas Enterococcus faecalis predominated within the MAT infant GIT. MAT infant mice subsequently exhibited increased and accelerated mortality following vaccinia virus infection. Ag-specific IFN-γ–producing CD8+ T cells were reduced in sublethally infected MAT infant mice. MAT CD8+ T cells from uninfected infant mice also demonstrated a reduced capacity to sustain IFN-γ production following in vitro activation. We additionally determined that control infant mice became more susceptible to infection if they were born in an animal facility using stricter standards of hygiene. These data indicate that undisturbed colonization and progression of the GIT microbiota during infancy are necessary to promote robust adaptive antiviral immune responses.

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Intestine-derived Clostridium leptum induces murine tolerogenic dendritic cells and regulatory T cells in vitro

Cited by 18 publications

References 37 publications

Microbiome–metabolomics reveals gut microbiota associated with glycine-conjugated metabolites and polyamine metabolism in chronic kidney disease

Microbiome–metabolomics reveals gut microbiota associated with glycine-conjugated metabolites and polyamine metabolism in chronic kidney disease

Impact of Selection for Digestive Efficiency on Microbiota Composition in the Chicken

Maternal Antibiotic Treatment Impacts Development of the Neonatal Intestinal Microbiome and Antiviral Immunity

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