Background: A gossypiboma is a pseudotumor caused by a foreign-body reaction developing around inadvertently retained surgical materials in the body. Although advancements in surgical techniques and equipment has enabled successful laparoscopic management for selected cases of gossypiboma, management of gossypiboma with laparoendoscopic single-site (LESS) surgery has never been described. Case: A 46-year-old, gravida 1, para 1 woman, who had a vaginal hysterectomy for a myoma 3 years prior, was referred due to a pelvic mass identified when she visited to her physician because of right back pain. Imaging showed a heterogeneous mass in the right adnexal region. She underwent transumbilical LESS surgery. Results: The transumbilical LESS surgery revealed that she had an encapsulated mass with bowel adhesion in the right adnexal region adjacent to ovarian tissue. When the capsular tissue was partially penetrated by careful blunt dissection, retained surgical gauze emerged and was extracted successfully without extension of the umbilical wound. The patient's postsurgical course was uneventful. Conclusions: A minimally invasive approach with LESS surgery could be a feasible option for management of gossypiboma in selected cases.