Intra-and Postdialytic Platelet Activation, Increased Platelet Phosphatidylserine Exposure and Ultrastructural Changes in Platelets in Children with Chronic Uremia

Elshamaa, Manal F.; Elghoroury, Eman A.; Helmy, Amira

doi:10.3923/jms.2007.319.329

Cited by 3 publications

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An elevated concentration of platelet factor-4 could be used as an indicator for platelet activation since they are usually released from alpha granules of activated platelets. Elshamaa et al [25] proved that PF-4 plays an important role in in ammation and wound repair and it is a strong chemo-attractant for neutrophils which is very crucial in the in ammatory process. Thus up-regulation and down-regulation of in ammation may be the reason for the non-signi cant increase at 2-month into therapy and decrease at 6-month into therapy.…”

Section: Discussionmentioning

confidence: 99%

Inflammation-mediated changes in haemostatic variables of pulmonary tuberculosis infected subjects in the course of treatment.

Okeke

Amilo

Manafa

et al. 2022

Preprint

View full text Add to dashboard Cite

Background Tuberculosis (TB) is a chronic infectious disease usually marked by a high level of inflammatory response. Haemostasis and inflammation are closely linked both in health and disease in such a way that inflammation leads to activation of the haemostatic system that in turn influences inflammatory activity. This prospective follow-up study was designed to assess inflammation-mediated changes in haemostatic variables of pulmonary tuberculosis infected subjects in the course of therapy. Method A total of 60 TB-infected individuals, aged 18-65 years participated in the study. The individuals were recruited before commencement of therapy and followed up to 6 month into therapy. Tuberculosis was diagnosed using Ziehl Neelsen acid-fast bacilli test and GeneXpert MTB/RIF assay. Pro-inflammatory cytokines: tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-2 (IL-2), anti-inflammatory cytokines: interleukin-10 (IL-10), transforming growth factor-beta (TGF-β) and haemostatic variables: thrombopoietin (TPO) P-selectin (P-SEL), platelet activating factor (PAF), platelet factor-4 (PF-4) and GP IIb/IIIa complex were measured by ELISA methods. These parameters were measured at pre-treatment, 2 month and 6 month into therapy. Data were analyzed using SPSS version 22. Results The results showed that TNF-α, IL-6, IL-2 (pro-inflammatory cytokines) and P-selectin, GP IIb/IIIa, thrombopoietin (haemostatic variables) were significantly increased at 2 month into therapy compared to pre-treatment values (p= <0.001, <0.001, <0.001, 0.015, 0.035 and 0.017 respectively) and decreased at 6 month into therapy. Also at 6 month into therapy in comparison to 2-month into therapy, there were significant increase in IL-10 and TGF-β (anti-inflammatory cytokines) (p=0.020 and 0.001 respectively) as well as a significant decline in PF-4 (p=0.030). There were significant positive correlations between GP IIb/IIIa and TNF-α (r=0.372; p=0.031), IL-6 and PSEL (r=0.413; p=0.027), IL-6 and TPO (r=0.335; p=0.046), PF4 and TGF-β (r=0.463; P=0.006), while PAF correlated negatively with TGF-β (r=-0.368; p=0.032). Conclusion The changes in the pro- inflammatory cytokines (TNF-α, IL-6, IL-2) aligned with changes in the levels of P-selectin, GP IIb/IIIa, and TPO in the course of TB therapy. This suggests that inflammation modulates the levels of these haemostatic variables in TB individuals.

show abstract

Section: Discussionmentioning

confidence: 99%