Intra-Articular Injection of Human Meniscus Stem/Progenitor Cells Promotes Meniscus Regeneration and Ameliorates Osteoarthritis Through Stromal Cell-Derived Factor-1/CXCR4-Mediated Homing

Shen, Wei; Chen, Jialin; Zhu, Ting; Chen, Longkun; Zhang, Wei; Fang, Zhi; Heng, Boon Chin; Yin, Zi; Chen, Xiao; Ji, Junfeng; Chen, Weishan

doi:10.5966/sctm.2012-0170

Cited by 96 publications

(116 citation statements)

References 47 publications

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“…However, they only tested the expression of CD34, CD45, CD44, CD90, CD105, and CD166 in human meniscus stem cells by flow cytometer without testing other stem cell markers, such as SSEA4, Nanog, Strol-1, nucleostemin, and CD73 (Shen et al 2013(Shen et al , 2014. To our knowledge, there is no such a report on investigation of the characterizations of meniscusderived stem cells with both flow cytometer and immunostaining.…”

Section: Discussionmentioning

confidence: 95%

A study to identify and characterize the stem/progenitor cell in rabbit meniscus

et al. 2016

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The repair of meniscus in the avascular zone remains a great challenge, largely owing to their limited healing capacity. Stem cells based tissue engineering provides a promising treatment option for damaged meniscus because of their multiple differentiation potential. We hypothesized that meniscusderived stromal cells (MMSCs) may be present in meniscal tissue, and if their pluripotency and character can be established, they may play a role in meniscal healing. To test our hypothesis, we isolated MMSCs, bone marrow-derived stromal cells (BMSCs) and fibrochondrocytes from rabbits. In order to avoid bone marrow mesenchymal stromal cell contamination, the parameniscal tissues and vascular zone of meniscus were removed. The characters of these three types of cells were identified by evaluating morphology, colony formation, proliferation, immunocytochemistry and multi-differentiation. Moreover, a wound in the center of rabbit meniscus was created and used to analyze the effect of BMSCs and MMSCs on wounded meniscus healing. BMSCs & MMSCs expressed the stem cell markers SSEA-4, Nanog, nucleostemin and STRO-1, while fibrochondrocytes expressed none of these markers. Morphologically, MMSCs displayed smaller cell bodies and larger nuclei than ordinary fibrochondrocytes. Moreover, it was certified that MMSCs and BMSCs were all able to differentiate into adipocytes, osteocytes, and chondrocytes in vitro. However, more cartilage formation was found in wounded meniscus filled with MMSCs than that filled with BMSCs. We showed that rabbit menisci harbor the unique cell population MMSCs that has universal stem cell characteristics and posses a tendency to differentiate into chondrocytes. Future research should investigate the mechanobiology of MMSCs and explore the possibility of using MMSCs to more effectively repair or regenerate injured meniscus.

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Section: Discussionmentioning

confidence: 95%

A study to identify and characterize the stem/progenitor cell in rabbit meniscus

et al. 2016

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“…Several lines of evidence revealed that CXCL12a/CXCR4 axis-mediated recruitment of MSCs is pivotal in damaged tissue repair in other disease conditions 7,15e18 . A recent in vivo study showed that the intra-articular injection of meniscus stem/progenitor cells promotes cartilage regeneration and ameliorates OA through CXCL12a/CXCR4 axismediated progenitor cell homing 19 . It is not clear, however, whether this protective effect of CXCL12a/CXCR4 axis directly depends on just the recruitment of MPCs or an additional paracrine effect of CXCL12a on chondrogenic differentiation of MPCs as well.…”

Section: Introductionmentioning

confidence: 99%

CXC chemokine ligand 12a enhances chondrocyte proliferation and maturation during endochondral bone formation

Kim

Han

Park

et al. 2015

Osteoarthritis and Cartilage

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“…Furthermore, approaches using cell therapy with tissue engineering and biomaterials are increasing [42].…”

Section: Cell Sourcementioning

confidence: 99%

Current Applications of Mesenchymal Stem Cells for Cartilage Tissue Engineering

Fuentes-Mera¹,

Camacho²,

Moncada‐Saucedo³

et al. 2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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Articular cartilage injuries caused by traumatic/mechanical progressive degeneration result in joint pain, swelling, the consequent loss of joint function, and eventually osteoarthritis. Articular tissue possesses a poor ability to regenerate that further complicates the therapeutic approaches. Mesenchymal stem cells (MSCs) have emerged as a promising alternative treatment. Recently, it has been reported that a wide variety of strategies ranging from merely using cells in the injured area to employ biofunctional substitutes in which cells are harmonizing with scafolding and growth factors to create an engineered cartilage tissue.This chapter reviews the state-of the-art in cartilage tissue engineering focused on tissue engineering approaches designed to recapitulate the native development of cartilage and its tridimensional structure as an osteochondral unit. Since the production of hypertrophied tissue is one of the most critical challenges to overcome in chondral tissue regeneration, here we show new strategies to minimize hypertrophy in cartilage. Finally, the eicacy and safety of diferent treatments of cartilage in current clinical trials will be discussed.While the framework provides new features and beneits concerning the strategies for articular tissue regeneration, this chapter presents a set of tools to improve approaches to orthopedic regenerative medicine based on the use of MSCs.Keywords: MSCs, MSCs-subpopulations, cartilage regeneration, cartilage tissue engineering, hypertrophy © 2017 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. IntroductionThe chondrocytes are the only cells found in cartilage. The chondrocytes demonstrate distinctive properties such as being metabolically active in order to maintain the renewal of the extracellular matrix (ECM) by synthesizing collagens, proteoglycans, hyaluronic acid, and glycoproteins. Restoration of the cartilage damage is still challenging for orthopedic medicine due to its poor ability to regenerate [1].Mesenchymal stem cells (MSCs) have potential applications in tissue engineering, and regenerative medicine represents an atractive option for repairing lesions in cartilage. Stem cellbased therapies that harmonize with tissue-engineering technologies, and biomaterials are vital for the continuous advance of cartilage regenerative medicine [2,3].Once the relationship between structure function in normal and damaged tissues is understood and the development of biological substitutes for the repair or regeneration can be reached. To develop a biological substitute, tissue engineering uses scafolds, cells, and growth factors. Each of these elements alone is able to promote tissue regeneration, but composites fabricated in combination would be more efective [4,5].The objective of the present chapter is, therefore, t...

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Intra-Articular Injection of Human Meniscus Stem/Progenitor Cells Promotes Meniscus Regeneration and Ameliorates Osteoarthritis Through Stromal Cell-Derived Factor-1/CXCR4-Mediated Homing

Cited by 96 publications

References 47 publications

A study to identify and characterize the stem/progenitor cell in rabbit meniscus

A study to identify and characterize the stem/progenitor cell in rabbit meniscus

CXC chemokine ligand 12a enhances chondrocyte proliferation and maturation during endochondral bone formation

Current Applications of Mesenchymal Stem Cells for Cartilage Tissue Engineering

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