With the recent advances in the area of OPC UA interfacing and the continuously growing requirements of the industrial automation world, combined with the more and more complex configurations of ECUs inside vehicles and services associated to car to infrastructure and even car to car communications, the gap between the two domains must be analyzed and filled. This gap occurred mainly because of the rigidness and lack of transparency of the software-hardware part of the automotive sector and the new demands for car to infrastructure communications. The issues are related to protocols as well as to conceptual views regarding requirements and already adopted individual directions. The industrial world is in the Industry 4.0 era, and in the Industrial Internet of Things context, its key interfacing enabler is OPC UA. Mainly to accommodate requirements related, among others, to high volumes, transfer rates, larger numbers of nodes, improved coordination and services, OPC UA enhances within its specifications the Publish-Subscribe mechanism and the TSN technology. In the OPC UA context, together with the VSOME/IP Notify-Subscribe mechanism, the current work is stepping toward a better understanding of the current relation between the needs of the industry and the suitable technologies, providing in-depth analysis on the most recent paradigms developed for data transmission, taking in consideration the real-time capabilities and use-cases of high concern in automation and automotive domains, and toward obtaining a VSOME/IP—OPC UA Gateway that includes the necessary characteristics and services in order to fill the protocol-related gap between the above mentioned fields. The developed case study results are proving the efficiency of the concept and are providing a better understanding regarding the impact between ongoing solutions and future requirements.