Intra‐clade metabolomic profiling of MAR4 Streptomyces from the Macaronesia Atlantic region reveals a source of anti‐biofilm metabolites

Abstract

Summary The search for new and effective strategies to reduce bacterial biofilm formation is of utmost importance as bacterial resistance to antibiotics continues to emerge. The use of anti‐biofilm agents that can disrupt recalcitrant bacterial communities can be an advantageous alternative to antimicrobials, as their use does not lead to the development of resistance mechanisms. Six MAR4 Streptomyces strains isolated from the Madeira Archipelago, at the unexplored Macaronesia Atlantic ecoregion, were used to study the chemical diversity of produced hybrid isoprenoids. These marine actinomycetes were investigated by analysing their crude extracts using LC–MS/MS and their metabolomic profiles were compared using multivariate statistical analysis (principal component analysis), showing a separation trend closely related to their phylogeny. Molecular networking unveiled the presence of a class of metabolites not previously described from MAR4 strains and new chemical derivatives belonging to the napyradiomycin and marinone classes. Furthermore, these MAR4 strains produce metabolites that inhibit biofilm formation of Staphylococcus aureus and Marinobacter hydrocarbonoclasticus. The anti‐biofilm activity of napyradiomycin SF2415B3 (1) against S. aureus was confirmed.

Metabolomic study of marine Streptomyces sp.: Secondary metabolites and the production of potential anticancer compounds

“…Moreover, GNPS hosts a public spectral library and many of its tools are able to automatically search for a spectral match. Molecular networking (GNPS), for instance, has been applied in screening processes for strain prioritization from large microbial libraries [23,24], in the differentiation of intra-clade bacteria [25], for investigation of microbial communication with co-cultivation experiments [26] and along with multivariate statistical analyses in studies of culture condition optimization for the production of specific metabolites, including OSMAC approach [3,27].…”

Identification of Constituents Affecting the Secretion of Pro-Inflammatory Cytokines in LPS-Induced U937 Cells by UHPLC-HRMS-Based Metabolic Profiling of the Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang

“…The combination of NMR- or MS- based metabolomics with supervised multivariate data analysis methods has been successfully applied in NPs research: For example, OPLS-DA has been used to identify discriminant markers in the 1 H-NMR and UHPLC-MS metabolic profiles of the closely related species Pelargonium sidoides and P. reniforme [18]; partial least squares discriminant analysis (PLS-DA) of UHPLC-TOF-MS data has been applied for the identification of chemical quality markers for different Ficus deltoidea varieties [19]. Correlating bioactivity data with metabolomic data has been successfully used to predict bioactive plant constituents which contribute to the activity of herbal extracts: For example, PLS-DA was used to predict the bioactive principles from 1 H-NMR metabolomic data of Galphimia glauca accessions with distinct in vivo sedative and anxiolytic activities [20]; recently, compounds with anti-biofilm activity were identified by correlating the LC-MS profiles of six marine Streptomyces strains with bioactivity data by means of PLS-DA [21].…”

The Essentials of Marine Biotechnology

“…The use of antifouling paints incorporating biocides like heavy metals and tributyltin was the chemical answer to the fouling issue, but these substances are known to leach into the water and pose deleterious effects to many non-target species (Yebra et al, 2004;Amara et al, 2018). Marine natural products, originating from bacteria as well as higher organisms, can represent an environmentally friendly alternative to synthetic biocides (Eguía and Trueba, 2007;Adnan et al, 2018;Bauermeister et al, 2019) and an economically advantageous solution to foul-release polymers (Trepos et al, 2014;Chen and Qian, 2017;Pereira et al, 2020). In particular, the compounds that inhibit quorum sensing signals that regulate the microbial colonization and formation of aggregates could mitigate the impact of biofilms (de Carvalho, 2018;Salehiziri et al, 2020).…”

“…Between 2007 and 2017, 177 new actinobacterial species belonging to 29 novel genera and three novel families were described (Subramani and Sipkema, 2019). The Gram-positive actinobacteria are a major chemically prolific source of bioactive metabolites with anticancer, antimicrobial, antiparasitic, anti-inflammatory, antibiofilm activities, and antifouling properties, among others (Prieto-Davó et al, 2016;Bauermeister et al, 2018Bauermeister et al, , 2019Cartuche et al, 2019Cartuche et al, , 2020Girão et al, 2019;Pereira et al, 2020). There are several marine-derived actinomycete metabolites described in the literature.…”