Intra-Colony Channel Morphology in Escherichia Coli Biofilms is Governed by Nutrient Availability and Substrate Stiffness

Bottura, Beatrice; Rooney, Liam M.; Hoskisson, Paul A.; McConnell, Gail

doi:10.2139/ssrn.4073174

Cited by 2 publications

(1 citation statement)

References 44 publications

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“…Furthermore, this functionality of our charge-neutral microgels has significant implications for investigating selection pressures in a generalized manner in 3D. For instance, substratum stiffness impacts the biofilm formation ability of bacteria [68][69][70][71][72][73], which may alter drug susceptibility. Phenotypic responses by microbes to variations in surface properties may also be linked to antibiotic resistance mechanisms.…”

Section: Jammed Agarose Microgels For Microbial Cell Culturementioning

confidence: 99%

Jammed microgel growth medium prepared by flash-solidification of agarose for 3D cell culture and 3D bioprinting

Sreepadmanabh

Ganesh

Bhat

et al. 2023

Preprint

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Although cells cultured in 3D platforms are proven to be beneficial for studying cellular behavior in settings similar to their physiological state, due to the ease, convenience, and accessibility, traditional 2D culturing approaches are widely adopted. Jammed microgels are a promising class of biomaterials well-suited for 3D cell culture, tissue bioengineering, and 3D bioprinting. However, existing protocols for fabricating such microgels either involve complex synthesis steps, long preparation times, or polyelectrolyte hydrogel formulations that sequester ionic elements from the cell growth media. Hence, there is an unmet need for a universally biocompatible, high-throughput, and widely accessible manufacturing process. We address these demands by introducing a rapid, high-throughput, and remarkably straightforward method to synthesize jammed microgels composed of flash-solidified agarose granules directly prepared in a culture medium of choice. Our jammed growth media are optically transparent, porous, yield stress materials with tunable stiffness and self-healing properties, which makes them ideal for 3D cell culture as well as 3D bioprinting. The charge-neutral and inert nature of agarose make them suitable for culturing various cell types and species, the specific growth media for which do not alter the chemistry of the manufacturing process. Unlike several existing 3D platforms, these microgels are readily compatible with standard techniques such as absorbance-based growth assays, antibiotic selection, RNA extraction, and live cell encapsulation. In effect, we present a versatile, highly accessible, inexpensive, and easily adoptable universal biomaterial for 3D cell culture and 3D bioprinting. We envision their widespread application not just in routine laboratory settings but also in designing multicellular tissue mimics and dynamic co-culture models of physiological niches.

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Section: Jammed Agarose Microgels For Microbial Cell Culturementioning

confidence: 99%

Jammed microgel growth medium prepared by flash-solidification of agarose for 3D cell culture and 3D bioprinting

Sreepadmanabh

Ganesh

Bhat

et al. 2023

Preprint

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Formation and emergent dynamics of spatially organized microbial systems

et al. 2023

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Spatial organization is the norm rather than the exception in the microbial world. While the study of microbial physiology has been dominated by studies in well-mixed cultures, there is now increasing interest in understanding the role of spatial organization in microbial physiology, coexistence and evolution. Where studied, spatial organization has been shown to influence all three of these aspects. In this mini review and perspective article, we emphasize that the dynamics within spatially organized microbial systems (SOMS) are governed by feedbacks between local physico-chemical conditions, cell physiology and movement, and evolution. These feedbacks can give rise to emergent dynamics, which need to be studied through a combination of spatio-temporal measurements and mathematical models. We highlight the initial formation of SOMS and their emergent dynamics as two open areas of investigation for future studies. These studies will benefit from the development of model systems that can mimic natural ones in terms of species composition and spatial structure.

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Intra-Colony Channel Morphology in Escherichia Coli Biofilms is Governed by Nutrient Availability and Substrate Stiffness

Cited by 2 publications

References 44 publications

Jammed microgel growth medium prepared by flash-solidification of agarose for 3D cell culture and 3D bioprinting

Jammed microgel growth medium prepared by flash-solidification of agarose for 3D cell culture and 3D bioprinting

Formation and emergent dynamics of spatially organized microbial systems

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