Intra‐organ biodistribution of gold nanoparticles using intrinsic two‐photon‐induced photoluminescence

Park, Jaesook; Estrada, Arnold D.; Schwartz, Jon A.; Diagaradjane, Parmeswaran; Krishnan, Sunil; Dunn, Andrew K.; Tunnell, James W.

doi:10.1002/lsm.20935

Cited by 38 publications

(30 citation statements)

References 45 publications

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“…2,26 In addition, their strong NIR plasmon peak (Figure 1) enables them to scatter, absorb or luminesce when excited in the NIR. 27,28 Recently, we demonstrated that systemically delivered GNSs passively accumulated in human colon tumors and served as excellent absorption-based contrast agents for wide-field tumor imaging. 29 Smaller particles have high permeation/diffusion through tumor interstitium and correspondingly, higher clearance to surrounding normal tissue (where they are likely to be cleared).…”

Section: Discussionmentioning

confidence: 99%

In vivo tumor targeting of gold nanoparticles: effect of particle type and dosing strategy

Puvanakrishnan

Park

Chatterjee

et al. 2012

IJN

103

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Gold nanoparticles (GNPs) have gained significant interest as nanovectors for combined imaging and photothermal therapy of tumors. Delivered systemically, GNPs preferentially accumulate at the tumor site via the enhanced permeability and retention effect, and when irradiated with near infrared light, produce sufficient heat to treat tumor tissue. The efficacy of this process strongly depends on the targeting ability of the GNPs, which is a function of the particle's geometric properties (eg, size) and dosing strategy (eg, number and amount of injections). The purpose of this study was to investigate the effect of GNP type and dosing strategy on in vivo tumor targeting. Specifically, we investigated the in vivo tumortargeting efficiency of pegylated gold nanoshells (GNSs) and gold nanorods (GNRs) for single and multiple dosing. We used Swiss nu/nu mice with a subcutaneous tumor xenograft model that received intravenous administration for a single and multiple doses of GNS and GNR. We performed neutron activation analysis to quantify the gold present in the tumor and liver. We performed histology to determine if there was acute toxicity as a result of multiple dosing. Neutron activation analysis results showed that the smaller GNRs accumulated in higher concentrations in the tumor compared to the larger GNSs. We observed a significant increase in GNS and GNR accumulation in the liver for higher doses. However, multiple doses increased targeting efficiency with minimal effect beyond three doses of GNPs. These results suggest a significant effect of particle type and multiple doses on increasing particle accumulation and on tumor targeting ability.

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Section: Discussionmentioning

confidence: 99%

In vivo tumor targeting of gold nanoparticles: effect of particle type and dosing strategy

Puvanakrishnan

Park

Chatterjee

et al. 2012

IJN

103

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“…Prior observations in PC-3 and other xenograft models using gadolinium based blood pool contrast agents [41] indicate that the peripheral cortex is more permeable than the tumor core which indicates that passive accumulation of the nanoshells is likely to be higher in these areas. This is in line with our observations on microscopy as well as spatial temperature profiles measured by MRTI.…”

Section: Discussionmentioning

confidence: 99%

MR temperature imaging of nanoshell mediated laser ablation

Stafford

Shetty²,

Elliott

et al. 2011

International Journal of Hyperthermia

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Minimally invasive thermal therapy using high-power diode lasers is an active area of clinical research. Gold nanoshells (AuNS) can be tuned to absorb light in the range used for laser ablation and may facilitate more conformal tumor heating and sparing of normal tissue via enhanced tumor specific heating. This concept was investigated in a xenograft model of prostate cancer (PC-3) using MR temperature imaging (MRTI) in a 1.5T scanner to characterize the spatiotemporal temperature distribution resulting from nanoparticle mediated heating . Tumors with and without intravenously injected AuNS were exposed to an external laser tuned to 808 nm for 180 sec at 4W/cm2 under real-time monitoring with proton resonance frequency shift based MRTI. Microscopy indicated that these nanoparticles (140–150 nm) accumulated passively in the tumor and remained close to the tumor microvasculature. MRTI measured a statistically significant (p<0.001) increase in maximum temperature in the tumor cortex (mean=21±7°C) in +AuNS tumors versus control tumors. Analysis of the temperature maps helped demonstrate that the overall distribution of temperature within +AuNS tumors was demonstrably higher versus control, and resulted in damage visible on histopathology. This research demonstrates that passive uptake of intravenously injected AuNS in PC-3 xenografts converts the tumor vasculature into a potent heating source for nanoparticle mediated ablation at power levels which do not generate significant damage in normal tissue. When used in conjunction with MRTI, this has implications for development and validation of more conformal delivery of therapy for interstitial laser ablations.

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“…According to recent reports, gold nanoparticles of 2-10 nm in diameter have low cellular uptake and renal clearance while large particles would be easily opsonized and ineffectively penetrate tumors via extravasation through fenestrations of vessels or by traveling through smaller terminal branches [37][38][39][40]. Pegylated HGNPs used for this study were designed to have average diameters of 52 nm, which has been known to show the highest cellular uptake [41][42][43], resulting in an absorption peak at 810 nm for maximum light penetration into tissue.…”

Section: Hgnp Characterizationmentioning

confidence: 99%

Intra‐organ biodistribution of gold nanoparticles using intrinsic two‐photon‐induced photoluminescence

Cited by 38 publications

References 45 publications

In vivo tumor targeting of gold nanoparticles: effect of particle type and dosing strategy

In vivo tumor targeting of gold nanoparticles: effect of particle type and dosing strategy

MR temperature imaging of nanoshell mediated laser ablation

Multifunctional hollow gold nanoparticles designed for triple combination therapy and CT imaging

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