Intra-species DNA polymorphism in the tobacco cyst  nematode complex (<i>Globodera</i> <i>tabacum</i>) using AFLP

Marché, Laurent; Valette, Sylvie; Grenier, Éric; Mugniéry, Didier

doi:10.1139/g01-091

Cited by 14 publications

(13 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…from other nematode species (15,24,34). The number of polymorphic loci is more important than the allelic diversity at each locus for the statistical power of analysis used for population assignment (7).…”

Section: Discussionmentioning

confidence: 99%

Ascariasis Is a Zoonosis in Denmark

Nejsum

Parker²,

Frydenberg³

et al. 2005

J Clin Microbiol

139

110

View full text Add to dashboard Cite

A preliminary epidemiological survey indicated an association between Ascaris infections in Danish patients and contact with pigs or pig manure. In the present study, we compared Ascaris worms collected from humans and Ascaris worms collected from pigs by amplified fragment length polymorphism (AFLP) analysis, a technique for whole-genome fingerprinting, and by PCR-linked restricted fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer region of nuclear rDNA. The AFLP data were analyzed by distance-and model-based clustering methods. These results assigned Ascaris worms from Danish patients to a cluster different from that for worms from humans in other geographic areas. In contrast, worms from humans and pigs in Denmark were assigned to the same cluster. These results were supported by the PCR-RFLP results. Thus, all of the examined Danish patients had acquired Ascaris infections from domestic pigs; ascariasis may therefore be considered a zoonotic disease in Denmark.

show abstract

Section: Discussionmentioning

confidence: 99%

Ascariasis Is a Zoonosis in Denmark

Nejsum

Parker²,

Frydenberg³

et al. 2005

J Clin Microbiol

139

110

View full text Add to dashboard Cite

show abstract

“…Examples include the randomly amplified polymorphic DNA (RAPD) (Cenis, 1993;CastagnoneSereno et al, 1994), amplified fragment length polymorphism (AFLP) (Semblat et al, 1998;Marché et al, 2001), restriction fragment length polymorphism (RFLP) (Curran et al, 1986;Carpenter et al, 1992) and sequence characterised amplified DNA regions (SCAR) (Zijlstra, 2000;Zijlstra et al, 2000;Carrasco-Ballesteros et al, 2007) (Table 2). These random DNA target-based markers have the advantage of having a higher multiplex ratio, a feature which is particularly useful when there is insufficient sequence divergence in the targeted DNA regions (Blok, 2005).…”

Section: Biochemical Methods For Nematode Identificationmentioning

confidence: 99%

Technological advancements and their importance for nematode identification

Ahmed

Sapp

Prior

et al. 2016

SOIL

View full text Add to dashboard Cite

Abstract. Nematodes represent a species-rich and morphologically diverse group of metazoans known to inhabit both aquatic and terrestrial environments. Their role as biological indicators and as key players in nutrient cycling has been well documented. Some plant-parasitic species are also known to cause significant losses to crop production. In spite of this, there still exists a huge gap in our knowledge of their diversity due to the enormity of time and expertise often involved in characterising species using phenotypic features. Molecular methodology provides useful means of complementing the limited number of reliable diagnostic characters available for morphology-based identification. We discuss herein some of the limitations of traditional taxonomy and how molecular methodologies, especially the use of high-throughput sequencing, have assisted in carrying out large-scale nematode community studies and characterisation of phytonematodes through rapid identification of multiple taxa. We also provide brief descriptions of some the current and almost-outdated high-throughput sequencing platforms and their applications in both plant nematology and soil ecology.

show abstract

“…ve Heterodera spp. popülasyonları arasındaki genetik ilişkilerin belirlenmesinde AFLP yönteminden yararlanılmaktadır (Xue et al, 1993;Folkerstma et al, 1996a,b;Jones & Harrower, 1998;Semblat et al, 1998;Van der Beek et al, 1998;Subbotin et al, 1999;Marché et al, 2001;Wang et al, 2001;Dautova et al, 2002). Türkiye'den elde edilen 29 farklı kök-ur nematodu izolatı arasındaki farklılıklar ve benzerlikler AFLP yöntemi ile ortaya koyulmuştur (Devran et al, 2008).…”

Section: Aflp (Amplified Fragment Length Polymorphism)unclassified

Nematod taksonomisinde kullanılan moleküler markörler

Gözel¹,

Yurt²,

Gözel³

2016

Türk. entomol. bült.

View full text Add to dashboard Cite

Morphological characters and morphometric measurements are used for diagnosis and identification of nematodes. In some cases, reliable results can not be achieved by classic methods. Therefore, molecular markers have been applied to nematode identification. Molecular markers are methods based on DNA. In this article, information regarding to the most common molecular marker techniques including Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), Microsatellites (SSR, STR, ISSR), Random Amplified Polymorphic DNA (RADP), Single Nucleotide Polymorphism (SNP) and DNA Sequence Analysis (mtDNA and rDNA) was given and the studies done by using these methods were reviewed.

show abstract

Intra-species DNA polymorphism in the tobacco cyst nematode complex (Globodera tabacum) using AFLP

Cited by 14 publications

References 13 publications

Ascariasis Is a Zoonosis in Denmark

Ascariasis Is a Zoonosis in Denmark

Technological advancements and their importance for nematode identification

Nematod taksonomisinde kullanılan moleküler markörler

Contact Info

Product

Resources

About

Intra-species DNA polymorphism in the tobacco cyst  nematode complex (Globodera tabacum) using AFLP

Cited by 14 publications

References 13 publications

Ascariasis Is a Zoonosis in Denmark

Ascariasis Is a Zoonosis in Denmark

Technological advancements and their importance for nematode identification

Nematod taksonomisinde kullanılan moleküler markörler

Contact Info

Product

Resources

About

Intra-species DNA polymorphism in the tobacco cyst nematode complex (Globodera tabacum) using AFLP