Intra-tracheal delivery of mesenchymal stem cell-conditioned medium ameliorates pathological changes by inhibiting apoptosis in asthmatic rats

Keyhanmanesh, ‬Rana; Khodamoradi, Fatemeh; Rahbarghazi‬, Reza; Rahbarghazi, Afshin; Rezaie, Jafar; Ahmadi, Mahdi; Salimi, Leila; Delkhosh, Aref

doi:10.1007/s11033-022-07212-8

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…Several studies have shown that intratracheal or intravenous delivery of MSC-conditioned medium significantly reduces pathological injuries in rats sensitized to ovalbumin (OVA) 64 , 82 , indicating that paracrine secretion is a critical mechanism by which MSCs exert immunomodulatory effects in asthma. Paracrine secretion involves the secretion of soluble factors and extracellular vesicle (EV) production.…”

Section: Msc-based Therapy For Asthmamentioning

confidence: 99%

Mesenchymal Stem/Stromal Cells in Asthma Therapy: Mechanisms and Strategies for Enhancement

Huang

Zeng

et al. 2023

Cell Transplant

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Asthma is a complex and heterogeneous disease characterized by chronic airway inflammation, airway hyperresponsiveness, and airway remodeling. Most asthmatic patients are well-established using standard treatment strategies and advanced biologicals. However, a small group of patients who do not respond to biological treatments or are not effectively controlled by available treatment strategies remain a clinical challenge. Therefore, new therapies are urgently needed for poorly controlled asthma. Mesenchymal stem/stromal cells (MSCs) have shown therapeutic potential in relieving airway inflammation and repairing impaired immune balance in preclinical trials owing to their immunomodulatory abilities. Noteworthy, MSCs exerted a therapeutic effect on steroid-resistant asthma with rare side effects in asthmatic models. Nevertheless, adverse factors such as limited obtained number, nutrient and oxygen deprivation in vitro, and cell senescence or apoptosis affected the survival rate and homing efficiency of MSCs, thus limiting the efficacy of MSCs in asthma. In this review, we elaborate on the roles and underlying mechanisms of MSCs in the treatment of asthma from the perspective of their source, immunogenicity, homing, differentiation, and immunomodulatory capacity and summarize strategies to improve their therapeutic effect.

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Section: Msc-based Therapy For Asthmamentioning

confidence: 99%

Mesenchymal Stem/Stromal Cells in Asthma Therapy: Mechanisms and Strategies for Enhancement

Huang

Zeng

et al. 2023

Cell Transplant

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“…However, cell therapy has some limitations regarding the maintenance of cell viability, functions, possibility of carcinogenic transformation, immunogenic rejection, and other safety concerns. Moreover, therapeutic potential of stem cells is mainly attributed to the paracrine effect of the cells that secrete different factors into their media (7) where MSC secretome was found to have anti-in ammatory and anti-apoptotic effects (9). So, stem cell secretome is becoming a promising novel cell free therapy for wound healing in regenerative medicine (7).…”

Section: Introductionmentioning

confidence: 99%

Photobiostimulation conjugated with stem cells or their secretome for treatment of temporomandibular joint arthritis; a comparative study

El-Qashty,

Elkashty,

Hany

2023

Preprint

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Background: Temporomandibular joint (TMJ) arthritis is a debilitating, challenging condition and different methods have been implicated for its treatment. This study aimed to test the therapeutic potentials of low-level laser therapy (LLLT) associated with adipose derived stem cells (ADSC) or their derived secretome on a murine model induced arthritis. Methods: Forty eight rats were divided into four groups where group I was the sham control, the rest of animals were subjected to arthritis induction using complete Freund’s adjuvant, then divided as follows: group II received phosphate buffered saline (PBS) intraarticular injection and irradiation of 0 j/cm2, group III received ADSCs derived secretome and irradiation of 38 j/cm2, and group IV received ADSCs and irradiation of 38 j/cm2 as well. One and three weeks after treatment, animals were euthanized and histological, histomorphometrical, histochemical, and immunohistochemical analysis were performed with the relevant statistical tests. Results: The arthritis group showed histological signs of joint injury including cartilage atrophy, articular disc fibrosis, irregular osteochondral interface, and condylar bone resorption together with high inflammatory reaction and defective proteoglycan content. In contrast, the treated groups III and IV showed much restoration of the joint structure with normal cartilage and disc thickness. The inflammation process was significantly suppressed especially after three weeks, and the cartilage proteoglycan content was much enhanced. No significant difference between the results of the two treated groups was detected. Conclusion: LLLT conjugated with ADSCs or ADSCs derived secretome can efficiently enhance the healing of arthritic TMJs. Stem cell secretome can be applied as a safe, potent therapy. However, further investigations are required to unravel its mechanism of action and pave its way as a safe, novel, cell free therapy.

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“…However, cell therapy has some limitations regarding the maintenance of cell viability, functions, possibility of carcinogenic transformation, immunogenic rejection, and other safety concerns. Moreover, therapeutic potential of stem cells is mainly attributed to the paracrine effect of the cells that secrete different factors into their media [ 11 ] where MSC secretome was found to have anti-inflammatory and anti-apoptotic effects [ 13 ]. So, stem cell secretome is becoming a promising novel cell free therapy for wound healing in regenerative medicine [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Photobiostimulation conjugated with stem cells or their secretome for temporomandibular joint arthritis in a rat model

El-Qashty,

Elkashty,

Hany

2023

BMC Oral Health

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Background Temporomandibular joint (TMJ) arthritis is a debilitating, challenging condition and different methods have been implicated for its treatment. This study aimed to test the therapeutic potentials of low-level laser therapy (LLLT) associated with adipose derived stem cells (ADSC) or their derived secretome on a murine model induced arthritis. Methods Forty eight rats were divided into four groups where group I was the sham control, the rest of animals were subjected to arthritis induction using complete Freund’s adjuvant, then divided as follows: group II received phosphate buffered saline (PBS) intraarticular injection and irradiation of 0 j/cm2, group III received ADSCs derived secretome and irradiation of 38 j/cm2, and group IV received ADSCs and irradiation of 38 j/cm2 as well. One and three weeks after treatment, animals were euthanized, and paraffin blocks were processed for histological assessment by hematoxylin and eosin stain with histomorphometrical analysis. Histochemical evaluation of joint proteoglycan content was performed through toluidine blue stain, and immunohistochemical staining by the proinflammatory marker tumor necrosis factor-α (TNF-α) was performed followed by the relevant statistical tests. Results The arthritis group showed histological signs of joint injury including cartilage atrophy, articular disc fibrosis, irregular osteochondral interface, and condylar bone resorption together with high inflammatory reaction and defective proteoglycan content. In contrast, the treated groups III and IV showed much restoration of the joint structure with normal cartilage and disc thickness. The inflammation process was significantly suppressed especially after three weeks as confirmed by the significant reduction in TNF-α positive immunostaining compared to the arthritic group, and the cartilage proteoglycan content also showed significant increase relative to the arthritic group. However, no significant difference between the results of the two treated groups was detected. Conclusion LLLT conjugated with ADSCs or ADSCs derived secretome can efficiently enhance the healing of arthritic TMJs. Stem cell secretome can be applied as a safe, potent therapy. However, further investigations are required to unravel its mechanism of action and pave its way as a safe, novel, cell free therapy. Graphical Abstract

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Intra-tracheal delivery of mesenchymal stem cell-conditioned medium ameliorates pathological changes by inhibiting apoptosis in asthmatic rats

Cited by 5 publications

References 24 publications

Mesenchymal Stem/Stromal Cells in Asthma Therapy: Mechanisms and Strategies for Enhancement

Mesenchymal Stem/Stromal Cells in Asthma Therapy: Mechanisms and Strategies for Enhancement

Photobiostimulation conjugated with stem cells or their secretome for treatment of temporomandibular joint arthritis; a comparative study

Photobiostimulation conjugated with stem cells or their secretome for temporomandibular joint arthritis in a rat model

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