Intrabacterial lipid inclusions

Dargham, Tonia; Mallick, Ivy; Raze, Dominique; Kremer, Laurent; Canaan, Stéphane

doi:10.1016/b978-0-323-91948-7.00003-8

Cited by 3 publications

(2 citation statements)

References 79 publications

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“…This raises questions about the accuracy of these subcellular localization studies. To overcome these limitations, the development of new technological modalities that allow to noninvasively investigate ILI-associated proteome is urgently needed [8]. The recent emergence of proximity labelling technologies has the potential to circumvent these limitations and offer further insights into the exact composition of the ILIomes [31].…”

Section: Identification Of Structural Motifs and Molecular Binding Fe...mentioning

confidence: 99%

“…These structures are also synthesized by prokaryotes, as intrabacterial lipid inclusions (ILI) [4][5][6][7], underscoring the widely conserved nature of these organelles among multiple kingdoms [1][2][3]. Lipid droplet and ILI are essentially composed of neutral lipids surrounded by an amphipathic monolayer of phospholipids with proteins that are dynamically interacting at their surface [6,8]. In addition to a common compositional architecture, they often share conserved proteins with multiple biological functions that are essential for their biogenesis, maintenance/integrity, and degradation [9].…”

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confidence: 99%

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Intrabacterial lipid inclusion‐associated proteins: a core machinery conserved from saprophyte Actinobacteria to the human pathogen Mycobacterium tuberculosis

Dargham,

Mallick,

Kremer

et al. 2023

FEBS Open Bio

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Mycobacterium tuberculosis (Mtb), the aetiologic agent of tuberculosis (TB), stores triacylglycerol (TAG) in the form of intrabacterial lipid inclusions (ILI) to survive and chronically persist within its host. These highly energetic molecules represent a major source of carbon to support bacterial persistence and reactivation, thus playing a leading role in TB pathogenesis. However, despite its physiological and clinical relevance, ILI metabolism in Mtb remains poorly understood. Recent discoveries have suggested that several ILI‐associated proteins might be widely conserved across TAG‐producing prokaryotes, but still very little is known regarding the nature and the biological functions of these proteins. Herein, we performed an in silico analysis of three independent ILI‐associated proteomes previously reported to computationally define a potential core ILI‐associated proteome, referred to as ILIome. Our investigation revealed the presence of 70 orthologous proteins that were strictly conserved, thereby defining a minimal ILIome core. We further narrowed our analysis to proteins involved in lipid metabolism and discuss here their putative biological functions, along with their molecular interactions and dynamics at the surface of these bacterial organelles. We also highlight the experimental limitations of the original proteomic investigations and of the present bioinformatic analysis, while describing new technological approaches and presenting biological perspectives in the field. The in silico investigation presented here aims at providing useful datasets that could constitute a scientific resource of broad interest for the mycobacterial community, with the ultimate goal of enlightening ILI metabolism in prokaryotes with a special emphasis on Mtb pathogenesis.

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Section: Identification Of Structural Motifs and Molecular Binding Fe...mentioning

confidence: 99%

mentioning

confidence: 99%

Intrabacterial lipid inclusion‐associated proteins: a core machinery conserved from saprophyte Actinobacteria to the human pathogen Mycobacterium tuberculosis

Dargham,

Mallick,

Kremer

et al. 2023

FEBS Open Bio

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Activity-based protein profiling in drug/pesticide discovery: Recent advances in target identification of antibacterial compounds

Chen,

Zhang,

Ding

et al. 2024

Bioorganic Chemistry

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The High Plasticity of Nonpathogenic Mycobacterium brumae Induces Rapid Changes in Its Lipid Profile during Pellicle Maturation: The Potential of This Bacterium as a Versatile Cell Factory for Lipid Compounds of Therapeutic Interest

Campo-Pérez

Guallar-Garrido

Luquin

et al. 2022

IJMS

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The immunomodulatory potential of mycobacteria to be used for therapeutic purposes varies by species and culture conditions and is closely related to mycobacterial lipid composition. Although the lipids present in the mycobacterial cell wall are relevant, lipids are mainly stored in intracellular lipid inclusions (ILIs), which have emerged as a crucial structure in understanding mycobacteria-host interaction. Little is known about ILI ultrastructure, production, and composition in nonpathogenic species. In this study, we compared the lipid profiles of the nonpathogenic immunomodulatory agent Mycobacterium brumae during pellicle maturation under different culture conditions with qualitative and quantitative approaches by using high-resolution imaging and biochemical and composition analyses to understand ILI dynamics. The results showed wax esters, mainly in early stages of development, and acylglycerols in mature ILI composition, revealing changes in dynamics, amount, and morphometry, depending on pellicle maturation and the culture media used. Low-glycerol cultures induced ILIs with lower molecular weights which were smaller in size in comparison with the ILIs produced in glycerol-enriched media. The data also indicate the simple metabolic plasticity of lipid synthesis in M. brumae, as well as its high versatility in generating different lipid profiles. These findings provide an interesting way to enhance the production of key lipid structures via the simple modulation of cell culture conditions.

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Intrabacterial lipid inclusions

Cited by 3 publications

References 79 publications

Intrabacterial lipid inclusion‐associated proteins: a core machinery conserved from saprophyte Actinobacteria to the human pathogen Mycobacterium tuberculosis

Intrabacterial lipid inclusion‐associated proteins: a core machinery conserved from saprophyte Actinobacteria to the human pathogen Mycobacterium tuberculosis

Activity-based protein profiling in drug/pesticide discovery: Recent advances in target identification of antibacterial compounds

The High Plasticity of Nonpathogenic Mycobacterium brumae Induces Rapid Changes in Its Lipid Profile during Pellicle Maturation: The Potential of This Bacterium as a Versatile Cell Factory for Lipid Compounds of Therapeutic Interest

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