Intracameral and Lenticular Penetration of Locally Applied Deuterium-Labeled Vitamin E in Rats

Possibility of Clinical Application of Vitamin E to Cataract Prevention

“…Nagata et al [78] reported that when a 1% vitamin E acetate solution, which was prepared by emulsifying vitamin E acetate with polysorbate 80, a detergent, was instilled to both eyes of rats with a single oral naphthalene administration 5 times a day everyday for 9 weeks and the effect of the instilled vitamin E acetate on cataract development was evaluated during the instillation period, this vitamin E acetate instillation retarded the progression rather than the formation of naphthalene-induced cataracts. In addition, Nagata et al [79] measured the concentrations of deuterium (D3)-labeled ␣-tocopherol acetate and D3-labeled ␣-tocopherol derived from D3-labeled ␣-tocopherol acetate in the aqueous humor and lens of rats after instilling a 1% D3-labeled ␣-tocopherol acetate solution prepared with polysorbate 80 into the cul-de-sac of both eyes of the rats at a dose of 5 l/eye 5 times a day for successive 1 and 3 weeks. As a result, ␣-tocopherol acetate was found to be penetrated into the aqueous humor and lens of rats by instillation of the emulsified ␣-tocopherol acetate solution to their eyes.…”