Intracavernosal Pressure Recording to Evaluate Erectile Function in Rodents

Pan, Feng; Zhang, Jie; Liu, Yuyan; Lu, Liangsheng; Qiu, Xuefeng; Lv, Kangtai; Zhang, Qipeng

doi:10.3791/56798

Cited by 10 publications

(9 citation statements)

References 21 publications

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“…RhoA and ROCK2 in rat cavernosum tissues showed decreased expression after intervention with HJI, but the expression of ROCK1 was not significantly affected by our treatment. As the most commonly used index for evaluating erectile function [32], the ICP indicated that HM and HH treatments did not fully improve erectile function to normal levels. HJI plays a role in the partial pathogenesis of BCNI-induced ED; however, HJI may achieve better therapeutic effects if it is combined with PDE5i drugs or other clinical treatments.…”

Section: Discussionmentioning

confidence: 99%

Effect of HongJing I in Treating Erectile Function and Regulating RhoA Pathway in a Rat Model of Bilateral Cavernous Nerve Injury

Zhao

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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HongJing I (HJI), a traditional Chinese herbal formula, has been confirmed to be effective for the clinical treatment of erectile dysfunction (ED). However, the mechanism of action of HJI remains unclear. Here, we aimed to investigate the effect and underlying mechanisms of HJI against ED in a rat model of bilateral cavernous nerve injury (BCNI). Rats were divided into five groups: normal control (NC), BCNI-induced ED model (M), M + low-dose HJI (HL), M + medium-dose HJI (HM), and M + high-dose HJI (HH). All groups were treated with normal saline or the relevant drug for 28 consecutive days after inducing BCNI-ED. At the end of the treatment period, the intracavernous pressure (ICP) was recorded, and histological examination was conducted using Masson’s trichrome staining. Immunofluorescence staining and western blotting were applied to detect the changes in fibrosis protein and Ras homolog A (RhoA), Rho-associated protein kinase 1 (ROCK1), and ROCK2 expression. We found that HJI effectively improved the ICP in the treatment groups. In addition, RhoA, ROCK1, and ROCK2 expression levels were increased upon BCNI-ED induction, and HJI successfully inhibited cavernosum fibrosis and the activation of RhoA/ROCK2 signaling. Overall, these results suggest that the effects of HJI in attenuating ED may be caused, at least in part, by the suppression of RhoA/ROCK2 signaling and alleviation of fibrosis. However, the precise mechanism surrounding this requires further investigation in future studies.

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Section: Discussionmentioning

confidence: 99%

Effect of HongJing I in Treating Erectile Function and Regulating RhoA Pathway in a Rat Model of Bilateral Cavernous Nerve Injury

Zhao

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…Erection function in rodents can be evaluated by measuring ICP [ 25 ]. The magnitude of erectile activity was quantified as the ratio of ICP to MAP, which is the common functional index to determine erectile function.…”

Section: Resultsmentioning

confidence: 99%

Effects of Cultured Cordycep militaris on Sexual Performance and Erectile Function in Streptozotocin‐Induced Diabetic Male Rats

Pohsa

Hanchang

Singpoonga

et al. 2020

BioMed Research International

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Cordyceps militaris (CM), a valuable edible and medicinal fungus, has been used as traditional medicine to treat health conditions, as well as hyposexuality in Asian societies for over a century. Due to the high demand, several artificial cultivation methods have been developed for their biological activities. In this study, CM was cultured on medium that contained white rice and silkworm pupae, and the levels of cordycepin and adenosine, as well as its aphrodisiac effects in diabetes-induced erectile dysfunction (DIED), were evaluated. Diabetic rats were induced by streptozotocin (STZ) injection and administered orally with CM (0.1, 0.5, and 1.0 g/kg BW/day) for 3 weeks. Diabetic rats in negative and positive control groups received vehicle and sildenafil citrate (5 mg/kg), respectively. Results showed the changes in mating behaviour in which mount latency and intromission latency were significantly increased in diabetic rats, compared with the normal control group. Diabetic rats also showed a significant reduction in intracavernosal pressure (ICP) response to cavernous nerve stimulation, sperm count, testosterone level, penile nitric oxide synthase (NOS), and testicular superoxide dismutase (SOD) activities, when compared to the normal control group. Administration of CM (0.1, 0.5, and 1.0 g/kg BW/day) reversed the effects of diabetes on the mating behaviour, and the ICP responses to electrical stimulation. Moreover, the levels of penile NOS, testicular SOD activities, testosterone, and sperm count were significantly increased, and testicular malondialdehyde (MDA) levels were significantly decreased in these treated diabetic rats. Diabetic rats treated with sildenafil showed a significant induction in intromission frequency and NOS and SOD activities, as well as a marked increase in ICP responses. These results suggest that CCM exerts its aphrodisiac effect, possibly through activating testosterone production and suppressing oxidative stress to enhance erectile function in diabetic rats.

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“…The mean arterial pressure (MAP) is used as a reference for ICP. Using ICP recording protocols, many key parameters of erectile function can be measured from the ICP response curve 15 . Figure 6 shows the mean maximum ICP ( Impairments of sperm quantity and quality after EHS onset.…”

Section: Increased Plasma Levels Of Stress Hormones Multiple Organ Dmentioning

confidence: 99%

Exertional heat stroke on fertility, erectile function, and testicular morphology in male rats

Lin

Huang

Tian

et al. 2021

Sci Rep

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The association of exertional heat stroke (EHS) and testicular morphological changes affecting sperm quality, as well as the association of EHS and hypothalamic changes affecting sexual behavior, has yet to be elucidated. This study aimed to elucidate the effects of EHS on fertility, erectile function, and testicular morphology in male rats. Animals were exercised at higher room temperature (36 ℃ relative humidity 50%) to induce EHS, characterized by excessive hyperthermia, neurobehavioral deficits, hypothalamic cell damage, systemic inflammation, coagulopathy, and multiple organ injury. In particular, EHS animals had erectile dysfunction (as determined by measuring the changes of intracavernosal pressure and mean arterial pressure in response to electrical stimulation of cavernous nerves). Rats also displayed testicular temperature disruption, poorly differentiated seminiferous tubules, impaired sperm quality, and atrophy of interstitial Leydig cells, Sertoli cells, and peri-tubular cells in the testicular tissues accompanied by no spermatozoa and broken cells with pyknosis in their seminal vesicle and prostatitis. These EHS effects were still observed after 3 days following EHS onset, at least. Our findings provide a greater understanding of the effect of experimentally induced EHS on masculine sexual behavior, fertility, stress hormones, and morphology of both testis and prostate.

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Intracavernosal Pressure Recording to Evaluate Erectile Function in Rodents

Cited by 10 publications

References 21 publications

Effect of HongJing I in Treating Erectile Function and Regulating RhoA Pathway in a Rat Model of Bilateral Cavernous Nerve Injury

Effect of HongJing I in Treating Erectile Function and Regulating RhoA Pathway in a Rat Model of Bilateral Cavernous Nerve Injury

Effects of Cultured Cordycep militaris on Sexual Performance and Erectile Function in Streptozotocin‐Induced Diabetic Male Rats

Exertional heat stroke on fertility, erectile function, and testicular morphology in male rats

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