Intracellular and extracellular sulphydryl levels in rheumatoid arthritis.

Koster, J.F.; Biemond, P.; Swaak, A. J. G.

doi:10.1136/ard.45.1.44

Cited by 183 publications

(113 citation statements)

References 8 publications

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“…The plasma content of the lipid peroxidation products, as fluorescent lipids and conjugated dienes, was estimated according to Ward et al [25]. The concentration of free SH groups in the plasma was measured with DTNB at 412 nm [26]. Plasma ferroxidase activity was measured according to Johnson et al [27].…”

Section: Other Biochemical Methodsmentioning

confidence: 99%

Blood glutathione redox status in gestational hypertension

Németh

Orvos

Boda

2001

Free Radical Biology and Medicine

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Abstract-Gestational hypertension during the third trimester reflects an exaggerated maternal inflammatory response to pregnancy. We hypothesized that oxidative stress present even in normal pregnancy becomes uncompensated in hypertensive patients. A glucose-6-phosphate dehydrogenase (G6PD) activity sufficient to meet the increased reductive equivalent need of the cells is indispensable for defense against oxidative stress. The erythrocyte glutathione redox system was studied, where G6PD is the only NADPH source. The glutathione (GSH) redox status was measured both in vivo and after an in vitro oxidative challenge in pregnant women with gestational hypertension (n ϭ 19) vs. normotensive pregnant subjects (n ϭ 18) and controls (n ϭ 20). An erythrocyte GSH depletion with an increase in the oxidized form (GSSG) resulted in an elevated ratio GSSG/GSH (0.305 Ϯ 0.057; mean Ϯ SD) in hypertensive pregnant women vs. normotensive pregnant or control subjects (0.154 Ϯ 0.025; 0.168 Ϯ 0.073; p Ͻ .001). In hypertensive pregnant patients, a "GSH stability" decrease after an in vitro oxidative challenge suggested a reduced GSH recycling capacity resulting from an insufficient NADPH supply. The erythrocyte GSSG/GSH ratio may serve as an early and sensitive parameter of the oxidative imbalance and a relevant target for future clinical trials to control the effects of antioxidant treatment in women at increased risk of the pre-eclampsia syndrome.

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Section: Other Biochemical Methodsmentioning

confidence: 99%

Blood glutathione redox status in gestational hypertension

Németh

Orvos

Boda

2001

Free Radical Biology and Medicine

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“…To determine lipid peroxidation, thiobarbituric acid reactive substances (TBARS) levels were determined according to a previously described method 11) . Oxidative damage to proteins were assessed by determinations of protein carbonyls 12) and total protein sulfhydryls (SH) levels 13) . Whole blood samples were used for erythrocyte reduced glutathione (GSH) measurements 14) .…”

Section: Biochemical Analysismentioning

confidence: 99%

Oxidant‐Antioxidant Status and Pulmonary Function in Welding Workers

Fidan¹,

Ünlü²,

Köken³

et al. 2005

Journal of Occupational Health

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Oxidant-Antioxidant Status and Pulmonary Function in Welding Workers: Fatma FIDAN, et al. Department of Pulmonary Diseases, Turkey-Welding is a process during which fumes, gases, electromagnetic radiation and noise are emitted as by-products. Metal oxide particles are particularly hazardous components of welding fumes. Welding has been found to be associated with respiratory symptoms and our objective in the present study was to study the effects of welding on pulmonary function and serum oxidant-antioxidant status. Fifty-one welding workers and 31 control subjects were recruited. Face to face interviews were conducted using the respiratory illness questionnaire adapted from the American Thoracic S o c i e t y w i t h t h e a d d i t i o n o f d e m o g r a p h i c characteristics, work history and working conditions. Additionally physical examinations and spirometric measurements were performed at workplaces. Thiobarbituric acid reactive substances (TBARS), protein carbonyls, protein sulfhydryls (SH) and erythrocyte reduced glutathione (GSH) levels were measured to evaluate oxidant-antioxidant status in 34 welding workers and in 20 control subjects. No statistically significant differences were observed in age, height, weight, body mass index (BMI), smoking status and annual working durations between welding workers and controls. Coughing, sputing and wheezing were significantly higher in welding workers (p<0.05). When adjusted for age, BMI and smoking status in logistic regression, welding work showed a significant risk for chronic bronchitis (OR: 4.78, 95%CI: 1.30-17.54). Forced expiratory volume in one second (FEV 1 )/forced vital capacity (FVC) and four parameters of forced expiratory flow (FEF: FEF 25 , FEF 50 , FEF 75 , FEF 25-75 ) levels measured in the welding workers were significantly lower than those in the control group (p<0.05). Serum TBARS and protein carbonyl levels were higher in welding workers than those in controls (p<0.001, p<0.05, respectively). On the other hand, total protein SH groups and GSH levels were significantly lower in welders than those in controls (p<0.05, p<0.001, respectively). Pulmonary function tests and oxidant-antioxidant status were found to be negatively affected in welding workers chronically exposed to welding fumes and gases. Preventive measures should be taken to improve the health status of these workers. (J Occup Health 2005; 47: 286-292)

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“…Values in mmol=g plasma protein were converted to mmol=l by multiplying by the plasma protein concentration. The concentration of serum protein sulphydryl groups was determined spectrophotometrically (Koster et al, 1986), and a blank for each sample was included.…”

Section: Laboratory Measurementsmentioning

confidence: 99%

The effect of meals rich in thermally stressed olive and safflower oils on postprandial serum paraoxonase activity in patients with diabetes

et al. 2001

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Objective: To determine the effects of meals rich in thermally stressed safflower (TSAF) and olive (TSOL) oils on postprandial serum paraoxonase (PON1) arylesterase activity and low density lipoprotein (LDL) oxidation in patients with type 2 diabetes. Design: A randomised cross-over study. Setting: Diabetes clinic and general practice. Subjects: Fourteen patients (six men and eight women) with type 2 diabetes, aged 48 -67 y, glycated haemoglobin < 10% and fasting blood glucose < 11 mmol=l were recruited. Interventions: Patients received a milkshake rich in TSAF or TSOL and at least a week later they received the alternate milkshake. These fats contained high levels of lipid oxidation and degradation products. Blood samples were taken fasted and 4 h after consumption of the milkshake. Main outcome measures: Serum PON1 activity and lag time in LDL oxidation. Results: After the meal rich in TSOL, serum PON1 activity increased significantly in women (12 (2.22) mmol=ml=min, mean (95% confidence interval), P ¼ 0.03) and not in men (0 (74.4) mmol=ml=min) during the postprandial period. The increase in PON1 activity after the TSOL meal was significantly (P ¼ 0.03) greater in women compared with men. In women, the increase in serum PON1 activity after the TSOL meal was significantly different (13 (1.25) mmol=ml=min, P ¼ 0.04) compared with the corresponding change (71 mmol=ml=min) after the TSAF meal. The lag time in LDL oxidation and indices of oxidative stress and antioxidant capacity did not vary significantly during the meals. Conclusions: Meals rich in TSOL may increase postprandial serum PON1 activity in middle-aged and older diabetic women. This change is potentially anti-atherogenic and may favour the use of olive oil over polyunsaturated fats in the diet of patients with type 2 diabetes. Sponsorship: The study was supported by a grant from the National Heart Foundation of New Zealand. Descriptors: paraoxonase; diabetes; oxidative stress; heated olive and safflower oils; postprandial European Journal of Clinical Nutrition (2001) 55, 951-958

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Intracellular and extracellular sulphydryl levels in rheumatoid arthritis.

Cited by 183 publications

References 8 publications

Blood glutathione redox status in gestational hypertension

Blood glutathione redox status in gestational hypertension

Oxidant‐Antioxidant Status and Pulmonary Function in Welding Workers

The effect of meals rich in thermally stressed olive and safflower oils on postprandial serum paraoxonase activity in patients with diabetes

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