Intracellular And Intercellular Transport Of Nitrogen And Carbon

Lohaus, Gertrud; Fischer, Karsten

doi:10.1007/0-306-48138-3_15

Cited by 10 publications

(13 citation statements)

References 177 publications

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“…of Rosaceae, Apiaceae, or Plantaginaceae) translocate Suc together with linear polyols. Also common plantain translocates Suc plus the linear polyol sorbitol (Lohaus and Fischer, 2002). RamspergerGleixner et al (2004) had shown a differential regulation for the genes encoding of the CC-specific Suc transporter PmSUC2 and the CC-specific polyol transporter PmPLT1 during the early stages of phloem development.…”

Section: Transformation Of Common Plantain With a Pmplt1mentioning

confidence: 99%

Common Plantain. A Collection of Expressed Sequence Tags from Vascular Tissue and a Simple and Efficient Transformation Method

Pommerrenig

Barth²,

Niedermeier³

et al. 2006

Plant Physiology

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The vascular tissue of higher plants consists of specialized cells that differ from all other cells with respect to their shape and size, their organellar composition, their extracellular matrix, the type of their plasmodesmata, and their physiological functions. Intact and pure vascular tissue can be isolated easily and rapidly from leaf blades of common plantain (Plantago major), a plant that has been used repeatedly for molecular studies of phloem transport. Here, we present a transcriptome analysis based on 5,900 expressed sequence tags (ESTs) and 3,247 independent mRNAs from the Plantago vasculature. The vascular specificity of these ESTs was confirmed by the identification of well-known phloem or xylem marker genes. Moreover, reverse transcription-polymerase chain reaction, macroarray, and northern analyses revealed genes and metabolic pathways that had previously not been described to be vascular specific. Moreover, common plantain transformation was established and used to confirm the vascular specificity of a Plantago promoter-b-glucuronidase construct in transgenic Plantago plants. Eventually, the applicability and usefulness of the obtained data were also demonstrated for other plant species. Reporter gene constructs generated with promoters from Arabidopsis (Arabidopsis thaliana) homologs of newly identified Plantago vascular ESTs revealed vascular specificity of these genes in Arabidopsis as well. The presented vascular ESTs and the newly developed transformation system represent an important tool for future studies of functional genomics in the common plantain vasculature.

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Section: Transformation Of Common Plantain With a Pmplt1mentioning

confidence: 99%

Common Plantain. A Collection of Expressed Sequence Tags from Vascular Tissue and a Simple and Efficient Transformation Method

Pommerrenig

Barth²,

Niedermeier³

et al. 2006

Plant Physiology

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“…After their synthesis in leaves by sugar phosphate reductases and polyol phosphate phosphatases, linear polyols are loaded into the phloem by polyol-H þ symporters that accumulate their substrates to concentrations of several hundred millimolar (Zimmermann and Ziegler, 1975;Lohaus and Fischer, 2002;G. Lohaus, unpublished data).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis POLYOL TRANSPORTER5, a New Member of the Monosaccharide Transporter-Like Superfamily, Mediates H⁺-Symport of Numerous Substrates, Including myo-Inositol, Glycerol, and Ribose

Geiger²,

et al. 2004

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Six genes of the Arabidopsis thaliana monosaccharide transporter-like (MST-like) superfamily share significant homology with polyol transporter genes previously identified in plants translocating polyols (mannitol or sorbitol) in their phloem (celery [Apium graveolens], common plantain [Plantago major], or sour cherry [Prunus cerasus]). The physiological role and the functional properties of this group of proteins were unclear in Arabidopsis, which translocates sucrose and small amounts of raffinose rather than polyols. Here, we describe POLYOL TRANSPORTER5 (AtPLT5), the first member of this subgroup of Arabidopsis MST-like transporters. Transient expression of an AtPLT5-green fluorescent protein fusion in plant cells and functional analyses of the AtPLT5 protein in yeast and Xenopus oocytes demonstrate that AtPLT5 is located in the plasma membrane and characterize this protein as a broad-spectrum H þ -symporter for linear polyols, such as sorbitol, xylitol, erythritol, or glycerol. Unexpectedly, however, AtPLT5 catalyzes also the transport of the cyclic polyol myo-inositol and of different hexoses and pentoses, including ribose, a sugar that is not transported by any of the previously characterized plant sugar transporters. RT-PCR analyses and AtPLT5 promoter-reporter gene plants revealed that AtPLT5 is most strongly expressed in Arabidopsis roots, but also in the vascular tissue of leaves and in specific floral organs. The potential physiological role of AtPLT5 is discussed.

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“…i tamo se prevode u SK, koji se skladišti u amiloplastu u obliku granula [2]. Veličina i oblik granula, kao i svojstva SK, zavise od vrste biljke koja ga sintetiše.…”

Section: Skrobunclassified

“…Shodno tome, za dobijanje polimera mogu da se koriste uobičajene metode polimerizacije. Za vinilne monomere koriste se 2 metode: (1) polimerizacija monomera u prisustvu polimera uz korišćenje inicijatora i kalemljenje rastućih lanaca na bočne grupe polimera, i (2) reakcije dva polimera od kojih jedan ima reaktivne grupu na jednom kraju lanca a drugi veći broj ortogonalnih bočnih reaktivnih grupa duţ svoga lanca. Prva metoda sigurno vodi dobijanju kalemljenih kopolimera.…”

Section: Slika 18 Strukture Razlicitih Tipova Kopolimeraunclassified

“…Ovaj proces moţe uključivati: (1) simultano ozračivanje polimera koji je u kontaktu sa monomerom; (2) ozračivanje polimera koji je prethodno ""uronjen"" u monomer; (3) prethodno ozračivanje polimera u odsustvu vazduha (O 2 ), praćeno izlaganjem monomeru; (4) prethodno ozračivanje polimera u prisustvu vazduha do formiranja peroksida i naknadni raspad u prisustvu monomera. Prve dve tehnike su poznate kao tehnike zajedničkog ozračivanja, za razliku od druge dve tehnike koje se obično nazivaju tehnikama prethodnog ozračivanja.…”

Section: Slika 18 Strukture Razlicitih Tipova Kopolimeraunclassified

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Copolumers obtained by grafting of vinyl monomers on starch - influence of amines on synthesis and degradation processes in environment

Nikolić¹

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Intracellular And Intercellular Transport Of Nitrogen And Carbon

Cited by 10 publications

References 177 publications

Common Plantain. A Collection of Expressed Sequence Tags from Vascular Tissue and a Simple and Efficient Transformation Method

Common Plantain. A Collection of Expressed Sequence Tags from Vascular Tissue and a Simple and Efficient Transformation Method

Arabidopsis POLYOL TRANSPORTER5, a New Member of the Monosaccharide Transporter-Like Superfamily, Mediates H⁺-Symport of Numerous Substrates, Including myo-Inositol, Glycerol, and Ribose

Copolumers obtained by grafting of vinyl monomers on starch - influence of amines on synthesis and degradation processes in environment

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Intracellular And Intercellular Transport Of Nitrogen And Carbon

Cited by 10 publications

References 177 publications

Common Plantain. A Collection of Expressed Sequence Tags from Vascular Tissue and a Simple and Efficient Transformation Method

Common Plantain. A Collection of Expressed Sequence Tags from Vascular Tissue and a Simple and Efficient Transformation Method

Arabidopsis POLYOL TRANSPORTER5, a New Member of the Monosaccharide Transporter-Like Superfamily, Mediates H+-Symport of Numerous Substrates, Including myo-Inositol, Glycerol, and Ribose

Copolumers obtained by grafting of vinyl monomers on starch - influence of amines on synthesis and degradation processes in environment

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Product

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Arabidopsis POLYOL TRANSPORTER5, a New Member of the Monosaccharide Transporter-Like Superfamily, Mediates H⁺-Symport of Numerous Substrates, Including myo-Inositol, Glycerol, and Ribose