Intracellular auxin transport in pollen

Bosco, Cristina Dal; Dovzhenko, Alexander; Palme, Klaus

doi:10.4161/psb.21953

Cited by 29 publications

(22 citation statements)

References 11 publications

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“…IAA exerted monotonous alkalization of the cytoplasm of pollen tubes and the strongest effect on F-actin assembly, thereby suggesting the involvement of this hormone in the regulation of AF organization through corresponding modulation of the activity of pH-sensitive actin binding proteins (ABPs). The auxin participation in the pollen tube polar growth was reported to be confirmed by PIN8 expression in the male gametophyte and its involvement in the regulation of intracellular auxin homeostasis [27]. Therefore, it can be concluded that auxin plays a key role in the maintenance of pollen tube polar growth that is in accordance with its similar behavior in other plant organs.…”

Section: Auxin Iaamentioning

confidence: 71%

Regulation of Petunia Pollen Tube Growth by Phytohormones: Identification of Their Potential Targets

Ковалева¹,

Воронков²,

Захарова³

et al. 2016

JAST-A

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Abstract:It is known that cytoskeleton-dependent trafficking of cell wall and membrane components to apical plasma membrane (PM) coupled with ion transport across pollen PM is crucial for maintaining polar pollen tube growth. To elucidate whether plant hormones are involved in these processes, the effects of exogenous phytohormones, indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellin A 3 (GA 3 ) and cytokinin (kinetin) on the growth, PM polarization, actin cytoskeleton (AC) organization and cytoplasmic pH (pH c ) of in vitro 4 h-growing petunia pollen tubes were investigated. IAA, ABA and GA 3 displayed the growth-stimulating effects and these were accompanied by orthovanadate-sensitive hyperpolarization of the PM. Fluorescent labeling the enzyme with H + -ATPase antibodies exhibited IAA-and ABA-induced lateral PM redistribution of it into the subapical zone of pollen tube PM. Pollen cultivation on the medium with latrunculin B, the inhibitor of actin polymerization, resulted in inhibition of pollen tube growth and simultaneously in the drop of endogenous IAA content. The IAA-growth stimulating effect was correlated with increased content of actin filaments (AF) in both apical and subapical zones of tubes, while ABA and GA 3 exerted the same effect but it was accompanied by redistributing F-actin only to apical zone. In contrast, kinetin decreased the total F-actin content and inhibited pollen tube growth. It has been shown that the рН c of growing pollen tubes is sensitive to the plant hormones. In the case of male gametophyte growing for 1, 2 and 4 h, IAA induced alkalinization of the cytosol, while ABA and GA 3 exerted qualitatively similar effect only after its growth for 1 h and 4 h, respectively. Kinetin, in contrast, resulted in acidification of the cytosol. All these results, taken together, indicate, for the first time, potential targets of the phytohormone action in pollen tubes.

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Section: Auxin Iaamentioning

confidence: 71%

Regulation of Petunia Pollen Tube Growth by Phytohormones: Identification of Their Potential Targets

Ковалева¹,

Воронков²,

Захарова³

et al. 2016

JAST-A

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“…1E, F), 27 which is consistent with a newly discovered function for PIN6 in nectary production and intracellular auxin transport in pollen. 25,28 …”

Section: Pin6 Is Required For Proper Auxin Response During Root Develmentioning

confidence: 98%

“…16 The ERlocalized PIN5, 6 and 8 proteins are involved in intracellular auxin distribution and homeostasis, where PIN5 and PIN8 are known to negatively regulate each other's transport capability, affecting pollen development and function. 19,21,25,26 The function of PIN6 as an auxin transporter, however, remained less obvious until very recent studies discovered essential roles for PIN6 in contributing Keywords: auxin, PIN6, transport, vasculature, hormone, development Cellular auxin homeostasis controls many aspects of plant growth, organogenesis and development. the existence of intracellular auxin transport mediated by endoplasmic reticulum (Er)-localized Pin5, Pin6 and Pin8 proteins is a relatively recent discovery shaping a new era in understanding auxin-mediated growth processes.…”

Section: Pin Mediated Polar Auxin Transport Defines Plant Developmentmentioning

confidence: 99%

The promoter of theArabidopsisPIN6 auxin transporter enabled strong expression in the vasculature of roots, leaves, floral stems and reproductive organs

Nisar

Cuttriss²,

Pogson

et al. 2014

Plant Signaling & Behavior

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Cellular auxin homeostasis controls many aspects of plant growth, organogenesis and development. The existence of intracellular auxin transport mediated by endoplasmic reticulum (ER)-localized PIN5, PIN6 and PIN8 proteins is a relatively recent discovery shaping a new era in understanding auxin-mediated growth processes. Here we summarize the importance of PIN6 in mediating intracellular auxin transport during root formation, leaf vein patterning and nectary production. While, it was previously shown that PIN6 was strongly expressed in rosette leaf cell types important in vein formation, here we demonstrate by use a PIN6 promoter-reporter fusion, that PIN6 is also preferentially expressed in the vasculature of the primary root, cotyledons, cauline leaves, floral stem, sepals and the main transmitting tract of the reproductive silique. The strong, vein- specific reporter gene expression patterns enabled by the PIN6 promoter emphasizes that transcriptional control is likely to be a major regulator of PIN6 protein levels, during vasculature formation, and supports the need for ER-localized PIN proteins in selecting specialized cells for vascular function in land plants.

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“…Interestingly, PIN5 showed enhanced expression in roots and both PIN5 and PIN8 showed decreased expression in inflorescences. PIN5 and PIN8 function to maintain intracellular auxin homeostasis for optimal pollen development and pollen tube growth possibly through negative effects on nuclear auxin signalling (Dal Bosco et al ., ,b; Ding et al ., ). Of the four members of the AUXIN IMPORT TRANSPORTER1 ( AUX1 ) and LIKE AUXIN RESISTANT ( LAX ) genes encoding auxin influx carriers, LAX1 showed increased mRNA abundance in leaves and inflorescences, whereas LAX3 showed decreased transcript abundance in inflorescences (Figure a and Tables S2, S3).…”

Section: Resultsmentioning

confidence: 97%

A Vitis vinifera basic helix–loop–helix transcription factor enhances plant cell size, vegetative biomass and reproductive yield

Lim

Yim

Liu

et al. 2018

Plant Biotechnology Journal

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SummaryStrategies for improving plant size are critical targets for plant biotechnology to increase vegetative biomass or reproductive yield. To improve biomass production, a codon‐optimized helix–loop–helix transcription factor (VvCEB1 opt) from wine grape was overexpressed in Arabidopsis thaliana resulting in significantly increased leaf number, leaf and rosette area, fresh weight and dry weight. Cell size, but typically not cell number, was increased in all tissues resulting in increased vegetative biomass and reproductive organ size, number and seed yield. Ionomic analysis of leaves revealed the VvCEB1 opt‐overexpressing plants had significantly elevated, K, S and Mo contents relative to control lines. Increased K content likely drives increased osmotic potential within cells leading to greater cellular growth and expansion. To understand the mechanistic basis of VvCEB1 opt action, one transgenic line was genotyped using RNA‐Seq mRNA expression profiling and revealed a novel transcriptional reprogramming network with significant changes in mRNA abundance for genes with functions in delayed flowering, pathogen–defence responses, iron homeostasis, vesicle‐mediated cell wall formation and auxin‐mediated signalling and responses. Direct testing of VvCEB1 opt‐overexpressing plants showed that they had significantly elevated auxin content and a significantly increased number of lateral leaf primordia within meristems relative to controls, confirming that cell expansion and organ number proliferation were likely an auxin‐mediated process. VvCEB1 opt overexpression in Nicotiana sylvestris also showed larger cells, organ size and biomass demonstrating the potential applicability of this innovative strategy for improving plant biomass and reproductive yield in crops.

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Intracellular auxin transport in pollen

Cited by 29 publications

References 11 publications

Regulation of Petunia Pollen Tube Growth by Phytohormones: Identification of Their Potential Targets

Regulation of Petunia Pollen Tube Growth by Phytohormones: Identification of Their Potential Targets

The promoter of theArabidopsisPIN6 auxin transporter enabled strong expression in the vasculature of roots, leaves, floral stems and reproductive organs

A Vitis vinifera basic helix–loop–helix transcription factor enhances plant cell size, vegetative biomass and reproductive yield

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