Intracellular Bacterial Biofilm-Like Pods in Urinary Tract Infections

Anderson, Gregory G.; Palermo, Joseph J.; Schilling, Joel D.; Roth, Robyn; Heuser, John E.; Hultgren, Scott J.

doi:10.1126/science.1084550

Cited by 1,016 publications

(916 citation statements)

References 29 publications

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“…UPEC have been shown to form biofilm-like structures (pods) in the urinary tract (30,31). By contrast, we made the observation that capsule-producing strains have a very limited ability to form biofilm in our microfermentor model.…”

Section: Discussionmentioning

confidence: 95%

Broad-spectrum biofilm inhibition by a secreted bacterial polysaccharide

Valle

Henry

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

238

208

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The development of surface-attached biofilm bacterial communities is considered an important source of nosocomial infections. Recently, bacterial interference via signaling molecules and surface active compounds was shown to antagonize biofilm formation, suggesting that nonantibiotic molecules produced during competitive interactions between bacteria could be used for biofilm reduction. Hence, a better understanding of commensal͞pathogen interactions within bacterial community could lead to an improved control of exogenous pathogens. To reveal adhesion or growthrelated bacterial interference, we investigated interactions between uropathogenic and commensal Escherichia coli in mixed in vitro biofilms. We demonstrate here that the uropathogenic strain CFT073 and all E. coli expressing group II capsules release into their environment a soluble polysaccharide that induces physicochemical surface alterations, which prevent biofilm formation by a wide range of Gram-positive and Gram-negative bacteria. We show that the treatment of abiotic surfaces with group II capsular polysaccharides drastically reduces both initial adhesion and biofilm development by important nosocomial pathogens. These findings identify capsular polymers as antiadhesion bacterial interference molecules, which may prove to be of significance in the design of new strategies to limit biofilm formation on medical in dwelling devices.bacterial interference ͉ Escherichia coli ͉ group II capsule ͉ extraintestinal

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Section: Discussionmentioning

confidence: 95%

Broad-spectrum biofilm inhibition by a secreted bacterial polysaccharide

Valle

Henry

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

238

208

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“…P pili contain an adhesin, PapG, that recognizes a globoside receptor in the human kidney as part of a mechanism important in the disease process (3,4). Type 1 pili contain the FimH adhesin that mediates E. coli binding, invasion, and formation of intracellular biofilm-like bacterial communities in the bladder epithelium (5,6).…”

mentioning

confidence: 99%

Rationally designed small compounds inhibit pilus biogenesis in uropathogenic bacteria

Pinkner

Remaut

Buelens

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

268

245

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A chemical synthesis platform with broad applications and flexibility was rationally designed to inhibit biogenesis of adhesive pili assembled by the chaperone-usher pathway in Gram-negative pathogens. The activity of a family of bicyclic 2-pyridones, termed pilicides, was evaluated in two different pilus biogenesis systems in uropathogenic Escherichia coli. Hemagglutination mediated by either type 1 or P pili, adherence to bladder cells, and biofilm formation mediated by type 1 pili were all reduced by Ϸ90% in laboratory and clinical E. coli strains. The structure of the pilicide bound to the P pilus chaperone PapD revealed that the pilicide bound to the surface of the chaperone known to interact with the usher, the outer-membrane assembly platform where pili are assembled. Point mutations in the pilicide-binding site dramatically reduced pilus formation but did not block the ability of PapD to bind subunits and mediate their folding. Surface plasmon resonance experiments confirmed that the pilicide interfered with the binding of chaperone-subunit complexes to the usher. These pilicides thus target key virulence factors in pathogenic bacteria and represent a promising proof of concept for developing drugs that function by targeting virulence factors.antimicrobials ͉ chaperone-usher pathway ͉ pilicide ͉ urinary tract infection

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“…It appears from light microscopy and EM data that the IBC closely associates with keratin intermediate filaments. 59,61 It was suggested that the intermediate filaments may provide scaffolds for the development of intracellular biofilms of UPEC. 61 The bacteria we visualized may reflect the early stage of the IBC development.…”

Section: Intermediate Filament Bundles Of the Umbrella Cells Provide mentioning

confidence: 99%

Atomic Force Microscopy of Mammalian Urothelial Surface

Kreplak

Wang

Aebi

et al. 2007

Journal of Molecular Biology

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SUMMARYThe mammalian urothelium apical surface plays important roles in bladder physiology and diseases, and it provides a unique morphology for ultrastructural studies. Atomic force microscopy (AFM) is an emerging tool for studying the architecture and dynamic properties of biomolecular structures in near physiological conditions. But AFM imaging of soft tissues remains a challenge due to the lack of efficient methods for sample stabilization. Using a porous nitrocellulose membrane as the support, we were able to immobilize large pieces of soft mouse bladder tissue thus enabling us to carry out the first AFM investigation of the mouse urothelial surface. The sub-micron resolution AFM images revealed many details of the surface features, including the geometry of the urothelial plaques that cover the entire surface and the membrane interdigitation at the cell borders. This interdigitation creates a membrane-zipper, likely contributing to the barrier function of the urothelium. In addition, we were able to image the intracellular bacterial communities (IBCs) of type 1-fimbriated bacteria grown between the intermediate filament bundles of the umbrella cells, shedding light on the bacterial colonization of urothelium.

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Intracellular Bacterial Biofilm-Like Pods in Urinary Tract Infections

Cited by 1,016 publications

References 29 publications

Broad-spectrum biofilm inhibition by a secreted bacterial polysaccharide

Broad-spectrum biofilm inhibition by a secreted bacterial polysaccharide

Rationally designed small compounds inhibit pilus biogenesis in uropathogenic bacteria

Atomic Force Microscopy of Mammalian Urothelial Surface

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