Intracellular Ca2+ homeostasis in rat round spermatids

Berríos, Julio; Osses, Nelson; Opazo, Carlos; Arenas, Gloria; Mercado, Luís; Benos, Dale J.; Reyes, Juan G.

doi:10.1016/s0248-4900(98)80088-9

Cited by 14 publications

(14 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, Sinkins et al [13] described results showing that MTX activation of Ca 2+ entry in cells was associated to PMCA expression and activation, strongly suggesting that this transport protein can be the target, or is closely associated to the target, of MTX action. PMCA is a Ca 2+ ‐CaM‐dependent transport ATPase regulated by ser/threo phosphorylation (e.g., [32]), expressed in spermatogenic cells [34,35]. In this context, our findings of a Ca 2+ and ATP‐dependent modulation of MTX‐activated Ca 2+ entry in spermatogenic cells can also be explained by this MTX‐induced PMCA‐ion channel association or conversion hypothesis (Sinkins et al, 2009) through the CaM activation of PMCA [33].…”

Section: Resultssupporting

confidence: 57%

Glucose and lactate regulate maitotoxin‐activated Ca²⁺ entry in spermatogenic cells: The role of intracellular [Ca²⁺]

et al. 2010

View full text Add to dashboard Cite

a b s t r a c tMaitotoxin (MTX), a potent polyether marine biotoxin, induces Ca 2+ entry in different mammalian cells by activation of Ca 2+ channels. The identity and modulation of the MTX-activated Ca 2+ entry pathway is not known. In this work, we show, for the first time, that glucose and lactate can modulate the excitability of spermatogenic cell MTX-activated Ca 2+ channels. Physiological and pharmacological evidences indicate that glucose and lactate differentially affect MTX-activated Ca 2+ entry mainly through changes that these substrates induce on intracellular Ca 2+ stores and the concentration of intracellular Ca 2+ ([Ca 2+ ] i ) in spermatogenic cells. Our findings strongly suggest that MTXactivated Ca 2+ channels in spermatogenic cells can be regulated by a Ca 2+ -CaM-dependent protein kinase.

show abstract

Section: Resultssupporting

confidence: 57%

Glucose and lactate regulate maitotoxin‐activated Ca²⁺ entry in spermatogenic cells: The role of intracellular [Ca²⁺]

et al. 2010

View full text Add to dashboard Cite

show abstract

“…In the absence of external Ca 2+ , addition of 50 µmol vanadate l -1 to the cells did not significantly affect the initial rate of basal cytosolic [Ca 2+ ] changes at the different temperatures (data not shown; for example see Berrios et al, 1998). This result indicates that under these conditions, the plasma membrane Ca 2+ -ATPase was contributing only marginally to intracellular Ca 2+ homeostasis in these cells.…”

Section: Temperature Dependence Of Intracellular Ca 2+ Fluxes In Pachmentioning

confidence: 81%

Temperature dependence of intracellular Ca2+ homeostasis in rat meiotic and postmeiotic spermatogenic cells

Herrera¹,

Salas²,

Lagos³

et al. 2001

Reproduction

View full text Add to dashboard Cite

The hypothesis that intracellular [Ca2+] is a cell parameter responsive to extreme temperatures in rat meiotic and postmeiotic spermatogenic cells was tested using intracellular fluorescent probes for Ca2+ and pH. In agreement with this hypothesis, extreme temperatures induced a rapid increase of cytosolic [Ca2+] in rat pachytene spermatocytes and round spermatids. Oscillatory changes in temperature can induce oscillations in cytosolic [Ca2+] in these cells. Intracellular [Ca2+] homeostasis in round spermatids was more sensitive to high temperatures compared with pachytene spermatocytes. The calculated activation energies for SERCA ATPase-mediated fluxes in pachytene spermatocytes and round spermatids were 62 and 75 kJ mol(-1), respectively. The activation energies for leak fluxes from intracellular Ca2+ stores were 55 and 68 kJ mol(-1) for pachytene spermatocytes and round spermatids, respectively. Together with changes in cytosolic [Ca2+], round spermatids undergo a decrease in pH(i) at high temperatures. This temperature-induced decrease in pH(i) appears to be partially responsible for the increase in cytosolic [Ca2+] of round spermatids induced by high temperatures. This characteristic of rat meiotic and postmeiotic spermatogenic cells to undergo an increment in cytosolic Ca2+ at temperatures > 33 degrees C can be related to the induction of programmed cell death by high temperatures in these cells.

show abstract

“…Previous studies mainly focused on the biochemical and electrophysiological properties of the calcium channels existing in the spermatogenic cells and the expression and localization pattern of the molecules related to calcium signaling, such as calmodulin [42]. Calmodulin is also highly expressed in the testis and displays a regulated expression pattern during spermatogenesis [43], [44], [45].…”

Section: Discussionmentioning

confidence: 99%

Iqcg Is Essential for Sperm Flagellum Formation in Mice

Tan

Chen

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

Mammalian spermatogenesis comprises three successive phases: mitosis phase, meiosis phase, and spermiogenesis. During spermiogenesis, round spermatid undergoes dramatic morphogenesis to give rise to mature spermatozoon, including the condensation and elongation of nucleus, development of acrosome, formation of flagellum, and removal of excessive cytoplasm. Although these transformations are well defined at the morphological level, the mechanisms underlying these intricate processes are largely unknown. Here, we report that Iqcg, which was previously characterized to be involved in a chromosome translocation of human leukemia, is highly expressed in the spermatogenesis of mice and localized to the manchette in developing spermatids. Iqcg knockout causes male infertility, due to severe defects of spermiogenesis and resultant total immobility of spermatozoa. The axoneme in the Iqcg knockout sperm flagellum is disorganized and hardly any typical (“9+2”) pattern of microtubule arrangement could be found in Iqcg knockout spermatids. Iqcg interacts with calmodulin in a calcium dependent manner in the testis, suggesting that Iqcg may play a role through calcium signaling. Furthermore, cilia structures in the trachea and oviduct, as well as histological appearances of other major tissues, remain unchanged in the Iqcg knockout mice, suggesting that Iqcg is specifically required for spermiogenesis in mammals. These results might also provide new insights into the genetic causes of human infertility.

show abstract

Intracellular Ca2+ homeostasis in rat round spermatids

Cited by 14 publications

References 23 publications

Glucose and lactate regulate maitotoxin‐activated Ca²⁺ entry in spermatogenic cells: The role of intracellular [Ca²⁺]

Glucose and lactate regulate maitotoxin‐activated Ca²⁺ entry in spermatogenic cells: The role of intracellular [Ca²⁺]

Temperature dependence of intracellular Ca2+ homeostasis in rat meiotic and postmeiotic spermatogenic cells

Iqcg Is Essential for Sperm Flagellum Formation in Mice

Contact Info

Product

Resources

About

Intracellular Ca2+ homeostasis in rat round spermatids

Cited by 14 publications

References 23 publications

Glucose and lactate regulate maitotoxin‐activated Ca2+ entry in spermatogenic cells: The role of intracellular [Ca2+]

Glucose and lactate regulate maitotoxin‐activated Ca2+ entry in spermatogenic cells: The role of intracellular [Ca2+]

Temperature dependence of intracellular Ca2+ homeostasis in rat meiotic and postmeiotic spermatogenic cells

Iqcg Is Essential for Sperm Flagellum Formation in Mice

Contact Info

Product

Resources

About

Glucose and lactate regulate maitotoxin‐activated Ca²⁺ entry in spermatogenic cells: The role of intracellular [Ca²⁺]

Glucose and lactate regulate maitotoxin‐activated Ca²⁺ entry in spermatogenic cells: The role of intracellular [Ca²⁺]