Intracellular calcium signalling patterns reflect the differentiation status of human T cells

Arrol, H. P.; Church, Leigh D; Bacon, P. A.; Young, Stephen P.

doi:10.1111/j.1365-2249.2008.03677.x

Cited by 21 publications

(17 citation statements)

References 48 publications

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“…Moreover, cell growth could influence [Ca 2+ ] i patterns as well. [Ca 2+ ] i oscillations could reflect cell differentiation status and mediate cell differentiation processes such as gene and protein expression . Peaks of [Ca 2+ ] i oscillations decreased rapidly and terminated with cell differentiation, and these data agreed with our results that more heterogeneous [Ca 2+ ] i oscillations occurred in 2–6th passage cells (early phase of differentiation), compare to 7–10th passage cells (late phase of differentiation).…”

Section: Discussionsupporting

confidence: 91%

Multiple calcium patterns of rat osteoblasts under fluidic shear stress

Meng

Sun

et al. 2018

Journal Orthopaedic Research

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The intracellular calcium ([Ca ] ) response induced by external forces can be diverse and complex. Using primary osteoblasts from Wistar rats, we found multiple patterns of [Ca ] responses induced by fluidic shear stress (Fss), including homogeneous non-oscillation and heterogeneous oscillations. These multiple-patterned [Ca ] responses could be influenced by Fss intensity, cell density, and cell differentiation. Our real-time measurements with free calcium, ATP, ATP without calcium, suramin, apyrase, and thapsigargin confirmed homogeneous [Ca ] patterns and/or heterogeneous [Ca ] oscillations with respect to the combined degree of external Ca influx, and intracellular Ca release. Our theoretical model supported diverse Fss-induced calcium activities as well. We concluded that a singular factor of Ca influx or release dominated to produce smooth homogeneous patterns, but combined factors produced oscillatory heterogeneous patterns. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:2039-2051, 2018.

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Section: Discussionsupporting

confidence: 91%

Multiple calcium patterns of rat osteoblasts under fluidic shear stress

Meng

Sun

et al. 2018

Journal Orthopaedic Research

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“…Ca 2+ signaling responses depend on the differentiation status of human T cells and drastically differ among individual cells, with some cells showing no Ca 2+ elevation at all (Arrol et al, 2008; Robert et al, 2013). The extents of PS exposure also vary both between the individual JkT-CCR5 cells in the culture (Figure S1) and between freshly isolated T lymphocytes, where activated/memory CD4+ T cells present the highest levels of cell surface PS (Elliott et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Fusion Stage of HIV-1 Entry Depends on Virus-Induced Cell Surface Exposure of Phosphatidylserine

Зайцева

Zaitsev

Melikov

et al. 2017

Cell Host & Microbe

120

132

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Summary HIV-1 entry into host cells starts with interactions between the viral envelope glycoprotein (Env) and cellular CD4 receptors and coreceptors. Previous work has suggested that efficient HIV entry also depends on intracellular signaling but this remains controversial. Here we report that formation of the pre-fusion Env–CD4–coreceptor complexes triggers non-apoptotic cell surface exposure of the membrane lipid phosphatidylserine (PS). HIV-1-induced PS redistribution depends on Ca2+ signaling triggered by Env-coreceptor interactions and involves the lipid scramblase TMEM16F. Externalized PS strongly promotes Env-mediated membrane fusion and HIV-1 infection. Blocking externalized PS or suppressing TMEM16F inhibited Env-mediated fusion. Exogenously added PS promoted fusion, with fusion dependence on PS being especially strong for cells with low surface density of coreceptors. These findings suggest that cell-surface PS acts as an important cofactor that promotes the fusogenic restructuring of pre-fusion complexes and likely focuses the infection on cells conducive to PS signaling.

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“…Calcium is a ubiquitous messenger in a number of physiological processes including immune cell response to antigen stimulation. 24 , 25 In addition, calcium oscillations have been observed in both excitable and nonexcitable cells, and differential immune response and gene expression have been attributed to varying calcium oscillation. 26 , 27 In general, the different calcium dynamics would affect cell signaling, ultimately leading to differences in cellular function.…”

Section: Resultsmentioning

confidence: 99%

A Generalizable, Tunable Microfluidic Platform for Delivering Fast Temporally Varying Chemical Signals to Probe Single-Cell Response Dynamics

et al. 2014

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Understanding how biological systems transduce dynamic, soluble chemical cues into physiological processes requires robust experimental tools for generating diverse temporal chemical patterns. The advent of microfluidics has seen the development of platforms for rapid fluid exchange allowing ease of changes in the cellular microenvironment and precise cell handling. Rapid exchange is important for exposing systems to temporally varying signals. However, direct coupling of macroscale fluid flow with microstructures is potentially problematic due to the high shear stresses that inevitably add confounding mechanical perturbation effects to the biological system of interest. Here, we have devised a method of translating fast and precise macroscale flows to microscale flows using a monolithically integrated perforated membrane. We integrated a high-density cell trap array for nonadherent cells that are challenging to handle under flow conditions with a soluble chemical signal generator module. The platform enables fast and repeatable switching of stimulus and buffer at low shear stresses for quantitative live, single-cell fluorescent studies. This modular design allows facile integration of any cell-handling chip design with any chemical delivery module. We demonstrate the utility of this device by characterizing heterogeneity of oscillatory response for cells exposed to alternating Ca2+ waveforms at various periodicities. This platform enables the analysis of cell responses to chemical perturbations at a single-cell resolution that is necessary in understanding signal transduction pathways.

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Intracellular calcium signalling patterns reflect the differentiation status of human T cells

Cited by 21 publications

References 48 publications

Multiple calcium patterns of rat osteoblasts under fluidic shear stress

Multiple calcium patterns of rat osteoblasts under fluidic shear stress

Fusion Stage of HIV-1 Entry Depends on Virus-Induced Cell Surface Exposure of Phosphatidylserine

A Generalizable, Tunable Microfluidic Platform for Delivering Fast Temporally Varying Chemical Signals to Probe Single-Cell Response Dynamics

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