Intracellular cytokine production by fetal and adult monocytes

Hebra, André; Strange, Phillip; Egbert, J. Michael; Ali, Mohamed R.; Mullinax, Ashley; Buchanan, Edward M.

doi:10.1053/jpsu.2001.26359

Cited by 28 publications

(20 citation statements)

References 22 publications

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“…However, for adhesion molecule expression and intracellular cytokine production, not all data in this paper are consistent with data in other papers about the same subject [3][4][5][6], including a previous paper by Luppi et al [7].…”

supporting

confidence: 89%

Monocyte cytokine production during pregnancy

Faas

Moes

Vos

2003

Journal of Leukocyte Biology

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With great interest, we have read the article, "Monocytes are progressively activated in the circulation of pregnant women" [1]. In their article, the authors showed that there is progressive, generalized, inflammatory cell activation, characterized by progressive up-regulation of CD11a, CD54, and CD64 during pregnancy. In addition, granulocyte numbers were increased, and lymphocyte numbers decreased during pregnancy.In this comprehensive paper, the authors not only showed phenotypical activation of the inflammatory cells but also functional activation of these cells, as they showed an increase in the percentage of monocytes spontaneously producing interleukin (IL)-12 in third-trimester pregnant women as compared with nonpregnant women and increased IL-1␤ production by stimulated second-trimester pregnant monocytes as compared with stimulated, nonpregnant monocytes. Moreover, stimulated third-trimester pregnant granulocytes produced more IL-8 than nonpregnant granulocytes.

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supporting

confidence: 89%

Monocyte cytokine production during pregnancy

Faas

Moes

Vos

2003

Journal of Leukocyte Biology

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“…Berner et al [27] observed no statistically significant differences in TNF-␣ and IL-6 concentrations in the supernatants of APBMNC and CBMNC after 24-hour coincubation with LPS. Hebra et al [28] showed that neonatal cord blood monocytes produce less TNF-␣ , but more IL-6 after LPS exposure than adult cells. Angelone et al [20] found that LPS-treated neonatal cord blood is characterized by a higher IL-6/TNF-␣ ratio than adult peripheral blood.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profiles of Adult Peripheral and Cord Blood Mononuclear Cells Altered by Lipopolysaccharide

et al. 2007

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Background: Neonatal Gram-negative sepsis is often characterized by a fulminant clinical course, compared to adults, resulting in higher morbidity and mortality. Genome-wide gene expression analysis can provide insights into the molecular alterations in sepsis. Objectives: To evaluate in vitro activation of the neonatal and adult immune system, gene expression patterns were compared in mononuclear cells from cord (CBMNC) and adult peripheral blood (APBMNC). Methods: To better understand the influence of early molecular signals on the effects of sepsis, Affymetrix gene profiling (8,475 genes) was done on RNA isolated from CBMNC and APBMNC without and after incubation with 100 ng/ml lipopolysaccharide (LPS). Results: We demonstrated significant alterations in the expression of 108 CBMNC and APBMNC genes compared with basal levels, 188 significant changes in CBMNC and 97 in APBMNC, including cytokines, chemokines and immunoregulatory genes. Furthermore, we found 5 genes showing a significant interaction effect between cell type and LPS stimulation, including tumor necrosis factor receptor superfamily, member 6 (FAS), absent in melanoma 2, malic enzyme 1, hemoglobin Ε 1, and trans-prenyltransferase. Conclusions: These results provide further support for a marked difference in the pathogenesis of neonatal and adult sepsis and may stimulate additional studies to investigate some of the altered genes as potential new targets for diagnostic tools and therapeutic strategies.

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“…Further studies have shown that the immune function in neonates is distinct from that in adults. For example, human neonatal cord blood monocytes have been reported to produce less interleukin (IL)-12 p70, interferon (IFN)-a, IFN-c, and tumor necrosis factor (TNF)-a, but as much or even more IL-1b, IL-6, IL-23, and IL-10 than adult human peripheral blood monocytes (PBMCs) in response to most Toll-like receptor (TLR) ligands including lipopolysaccharide (LPS), a major component of Gram-negative bacterial cell wall and a potent immune activator [3][4][5][6][7]. Polymorphonuclear leukocytes (PMNs) from preterm infants have been reported to exhibit a much reduced antibacterial activity from those from adults [8].…”

Section: Introductionmentioning

confidence: 99%