Intracellular Development and Mechanism of Hemadsorption of a Human Coronavirus, OC43

Bucknall, R. A.; Kalica, Anthony R.; Chanock, Robert M.

doi:10.3181/00379727-139-36243

Cited by 20 publications

(12 citation statements)

References 3 publications

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“…The host range of agglutinated and adsorbed erythrocytes as well as gradation in sensitivity and optimal temperature for agglutination of tested erythrocytes with the OC 43 strain are similar to the results obtained with the VW572 hemagglutination and hemadsorption experiments. On the other hand, since hemadsorption kept stable after twofold washing of the adsorbing cell sheet, it is thought not to be a "pseudohemadsorption", due to accumulation of newly formed virus particles at the external cell surface, as described by B~CK~ALL et al (4). The occurrence of hemadsorption may be an indication for an altered structure of the cell membrane, as proposed by ME~'GELING et al (22).…”

Section: Discussionmentioning

confidence: 86%

Characteristics of a coronavirus causing vomition and wasting in pigs

Pensaert

Callebaut

1974

Archiv f Virusforschung

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Some characteristics of a virus, isolated from the tonsils of 2 pigs with clinical signs of inappetenee and vomition and designated VW572 were examined. It induced the formation of syneytia in primary pig kidney cell cultures, caused hemadsorption and hemagglutination using chicken, turkey, rat and mouse erythrocytes. In growth curve experiments, infectious virus was produced intracellularly starting at 6 hours after inoculation and was followed by rapid release of the virus from the infected cells. The virus contains ribonucleic acid, is ether sensitive and has a size between 100 and 220 nm. At 37 ~ C, the infectivity titer decreased about 2 lOgl0TCIDs0 per 24 hours. The viral population was heterogeneous as indicated by the rate of inactivation by U.V. irradiation and acid pH. Some hemagglutinating activity was left after complete loss of infectivity by ether-, temperature-and U.V. treatment. The VW 572 isolate is antigenically related if not identical to isolates from Canada, U.S.A. and England which were classified as a porcine eoronavirus.Oronasal and intracerebral inoculation of the VW572 isolate in colostrum deprived pigs resulted in clinical disease after an incubation period of 6 and 4 days, respectively. Signs were characterized by depression, vomition, loss of appetence with rapid weakness in young pigs and vomition with progressive wasting in older pigs. Virus was isolated from nasal and pharyngeal swabs, nasal mueosa, tonsils, lungs and hind brains but not from rectal swabs or other organs tested. Virus could not be isolated later than 8 days after inoculation. Hemagglutination inhibiting and neutralizing antibodies were detected in sera at 6 days and 9 days after inoculation, respectively.

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Section: Discussionmentioning

confidence: 86%

Characteristics of a coronavirus causing vomition and wasting in pigs

Pensaert

Callebaut

1974

Archiv f Virusforschung

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“…Coronaviruses are enveloped viruses containing a single-stranded genomic RNA of positive polarity. The human coronavirus OC43 [4,10,11,22,28,33] and bovine coronavirus (BCV) [3,14,20,22,28,29] are known to possess both haemagglutinating (HA) and receptor-destroying enzyme (RDE) activities similar to influenza C viruses [6,9,12,21,27]. The diarrhea virus of infant mice (DVIM) is an enterotropic mouse hepatitis virus (MHV) that was originally isolated and characterized by Sato and coworkers [15].…”

Section: Introductionmentioning

confidence: 99%

Haemagglutinin-esterase protein (HE) of murine corona virus: DVIMD (diarrhea virus of infant mice)

et al. 1998

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The acetylesterase (AE) activity of DVIM (diarrhea virus of infant mice) was assigned to the haemagglutinin-esterase (HE) protein. The substrate specificity was examined using the natural substrate bovine submaxillary mucin (BSM) and/or synthetic substrates p-nitrophenylacetate (p-NiA) and alpha-naphthylacetate (alpha-NA) and compared with several strains of MHV and influenza viruses. The AE of DVIM hydrolyzed the O-acetylester bond of BSM, and the two synthetic substrates p-NiA and alpha-NA in vitro. MHV-S reacted efficiently with both p-NiA and alpha-NA but less with BSM. Influenza virus (C/Miyagi/77) reacted with BSM efficiently, however reacted with p-NiA weakly, but not with alpha-NA at all. Thus, the AE-reactivity of DVIM was distinctly different from that of MHV-S and influenza C virus, suggesting that the AE of HE may have a modified function. Isolation of HE by the treatment with non ionic detergent NP40, resulted in globules approximately 5 nm in diameter. DVIM-binding proteins were demonstrated in the plasma membrane of mouse intestinal brush-border cells and hepatocytes. The same protein was recognized by MHV-S and MHV-4. The cell membranes obtained from these target tissues were substrates for the AE of DVIM. The biological importance of the HE protein for DVIM is discussed.

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“…Coronaviruses are enveloped viruses with singlestranded positive-sense RNA genomes approximately 30 kb [4]. Most viruses in coronavirus family cause diseases in animal, while a few of them; such as HCoV-229E, HCoV OC43, HCoV-NL-63, and SARS-CoV are human pathogens [5][6][7]. Among all human coronaviruses, SARS-CoV is the only one that causes severe clinical consequences.…”

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confidence: 99%

Potent and selective inhibition of SARS coronavirus replication by aurintricarboxylic acid

Adonov

Traykova-Adonova

et al. 2004

Biochemical and Biophysical Research Communications

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The severe acute respiratory syndrome virus (SARS) is a coronavirus that instigated regional epidemics in Canada and several Asian countries in 2003. The newly identified SARS coronavirus (SARS-CoV) can be transmitted among humans and cause severe or even fatal illnesses. As preventive vaccine development takes years to complete and adverse reactions have been reported to some veterinary coronaviral vaccines, anti-viral compounds must be relentlessly pursued. In this study, we analyzed the effect of aurintricarboxylic acid (ATA) on SARS-CoV replication in cell culture, and found that ATA could drastically inhibit SARS-CoV replication, with viral production being 1000-fold less than that in the untreated control. Importantly, when compared with IFNs alpha and beta, viral production was inhibited by more than 1000-fold as compared with the untreated control. In addition, when compared with IFNs alpha and beta, ATA was approximately 10 times more potent than IFN alpha and 100 times more than interferon beta at their highest concentrations reported in the literature previously. Our data indicated that ATA should be considered as a candidate anti-SARS compound for future clinical evaluation.

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Intracellular Development and Mechanism of Hemadsorption of a Human Coronavirus, OC43

Cited by 20 publications

References 3 publications

Characteristics of a coronavirus causing vomition and wasting in pigs

Characteristics of a coronavirus causing vomition and wasting in pigs

Haemagglutinin-esterase protein (HE) of murine corona virus: DVIMD (diarrhea virus of infant mice)

Potent and selective inhibition of SARS coronavirus replication by aurintricarboxylic acid

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