Intracellular Distribution of Hepatic and Renal Gluconeogenic Enzymes in Embryonic and Growing Chickens

Shen, C. S.; Mistry, S. P.

doi:10.3382/ps.0581239

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…These parallel changes of serum glucose and hepatic glycogen were also confirmed in chick embryos ( Latour et al, 1996 ; Christensen et al, 2001 ; Roy et al, 2013a ). The rate-limiting enzymes of PC, PCK, PFKFB2, and G6PC1 are responsible for the irreversible catalytic reactions in gluconeogenesis ( Shen and Mistry, 1979 ; van Schaftingen and Gerin, 2002 ; Chesney, 2006 ). Hepatic gene mRNA expression of PRKAA1, PC, PCK, PFKFB2, and G6PC1, and the relative protein expression of G6PC1 increased from E16 to hatch, and the maximal values were observed between E22 and E31.…”

Section: Discussionmentioning

confidence: 99%

Exogenous Linoleic Acid Intervention Alters Hepatic Glucose Metabolism in an Avian Embryo Model

Zhang

Zheng

et al. 2022

Front. Physiol.

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In the present study, developmental changes of gluconeogenesis and glycolysis in an avian model were measured, and then the intervention effects of in ovo feeding (IOF) linoleic acid (LA) on hepatic glucose metabolism were evaluated. In Experiment 1, thirty fertilized eggs were sampled on embryonic days (E) of 16, 19, 22, 25, 28, 31, and thirty newly-hatched ducklings at hatch (E34 and E35). In Experiment 2, a total of 120 fertilized eggs (60 eggs for each group) were injected into the yolk sac with PBS as the control group and LA as the IOF LA group on E25. Twelve eggs were selected for sample collection on E28 and E31. Serum contents of glucose, pyruvate, and lactate increased ( p < 0.05) linearly or quadratically from E16 to hatch, as well as hepatic glycogen and pyruvate contents. Hepatic mRNA expression related to energy homeostasis, gluconeogenesis, and glycolysis increased ( p < 0.05) in embryogenesis, and the plateau period was presented on E25–E31. IOF LA decreased ( p < 0.05) serum contents of glucose, triacylglycerol, cholesterol, and hepatic oleic acid, unsaturated fatty acids on E28, as well as the gene expression relative to gluconeogenesis. IOF LA increased ( p < 0.05) pyruvate content in serum and liver, and hepatic gene expression relative to glycolysis on E31. In summary, hepatic gluconeogenesis and glycolysis were enhanced to meet the increasing energy demands of embryonic development during E25 – hatch. Exogenous LA intervention on E25 could inhibit hepatic gluconeogenesis and enhance glycolysis during the later developmental period, disrupting glucose embryonic homeostasis and energy status.

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Section: Discussionmentioning

confidence: 99%

Exogenous Linoleic Acid Intervention Alters Hepatic Glucose Metabolism in an Avian Embryo Model

Zhang

Zheng

et al. 2022

Front. Physiol.

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A Technique for the Isolation of Chicken Hepatocytes and Their Use in a Study of Gluconeogenesis

Schultz

Mistry

1981

Poultry Science

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A new technique was developed for the isolation of chicken liver parenchymal cells. Glucose produced from 10 mM lactate was proportional to the amount of cells present. In the time-course study, gluconeogenesis from lactate and fructose was linear up to 60 min. Fructose proved to be the best substrate. Fructose was converted to glucose at the highest rate; this was followed by lactate, pyruvate, and xylitol. Alanine, glycerol, propionate, alpha-ketoglutarate, and succinate proved to be poor substrates. There was no statistical difference between the results obtained with hepatocytes obtained from fed or fasted chickens. The isolated hepatocytes responded to glucagon, dibutyryl-cAMP, and epinephrine. The dose-response for glucagon was a sigmoid-curve and the half-maximum stimulation was given by approximately 1 x 10(-2) micrometers hormone. The same type of curve was obtained with dibutyryl-cAMP, but the half-maximum stimulation was achieved at around 1.0 micrometer. The response to epinephrine was marginal. In the time-course experiment, prior to glucagon stimulation, glucose accumulated at a linear rate (slope = .2484). After the addition of the hormone, the level of cAMP increased by about 30% in the first minute and reached a peak (100%) in about 2 min; thereafter, it decreased to the level prior to the stimulation by the hormone. Two minutes after the addition of glucagon there was a significant increase in the rate of gluconeogenesis; this continued for another 3 min and then at a slower pace (slope = .2566).

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Intracellular Distribution of Hepatic and Renal Gluconeogenic Enzymes in Embryonic and Growing Chickens

Cited by 2 publications

References 40 publications

Exogenous Linoleic Acid Intervention Alters Hepatic Glucose Metabolism in an Avian Embryo Model

Exogenous Linoleic Acid Intervention Alters Hepatic Glucose Metabolism in an Avian Embryo Model

A Technique for the Isolation of Chicken Hepatocytes and Their Use in a Study of Gluconeogenesis

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