2018
DOI: 10.1111/1750-3841.14117
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Intracellular Expression of Antifreeze Peptides in Food Grade Lactococcus lactis and Evaluation of Their Cryoprotective Activity

Abstract: The recombinant Lactococcus lactis with intracellular expression of antifreeze peptides SF-P could reduce the damage of bacteria cells induced by freezing or freeze drying, so, it could be applied in the process of freezing food without separation, such as the manufacture of yoghurt ice cream, frozen dough, and so on.

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Cited by 13 publications
(11 citation statements)
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“…It was reported that upon the addition of nisin peptide concentrations greater than 20 ng ml Ϫ1 , the LAC240 cells became stressed, resulting in a reduction in the quantity of GFP produced, and the signal reached the background level when the concentration of nisin was approximately 60 ng ml Ϫ1 (26). Moreover, in a study that aimed to improve the response of L. lactis to freezing damage through expression of an antifreeze peptide (SF-P), the recombinant strain L. lactis NZ3900 SF-P was incubated with different concentrations of nisin (25, 50, or 100 ng ml Ϫ1 ) and at various pHs and growth temperatures (27). Notably, maximal expression was observed at 25 ng ml Ϫ1 , with a much lower level of expression at 50 ng ml Ϫ1 and virtually no expression at 100 ng ml Ϫ1 , most likely due to the inhibitory effects of nisin A, though pH and temperature values were also a factor (27).…”
Section: Discussionmentioning
confidence: 99%
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“…It was reported that upon the addition of nisin peptide concentrations greater than 20 ng ml Ϫ1 , the LAC240 cells became stressed, resulting in a reduction in the quantity of GFP produced, and the signal reached the background level when the concentration of nisin was approximately 60 ng ml Ϫ1 (26). Moreover, in a study that aimed to improve the response of L. lactis to freezing damage through expression of an antifreeze peptide (SF-P), the recombinant strain L. lactis NZ3900 SF-P was incubated with different concentrations of nisin (25, 50, or 100 ng ml Ϫ1 ) and at various pHs and growth temperatures (27). Notably, maximal expression was observed at 25 ng ml Ϫ1 , with a much lower level of expression at 50 ng ml Ϫ1 and virtually no expression at 100 ng ml Ϫ1 , most likely due to the inhibitory effects of nisin A, though pH and temperature values were also a factor (27).…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, in a study that aimed to improve the response of L. lactis to freezing damage through expression of an antifreeze peptide (SF-P), the recombinant strain L. lactis NZ3900 SF-P was incubated with different concentrations of nisin (25, 50, or 100 ng ml Ϫ1 ) and at various pHs and growth temperatures (27). Notably, maximal expression was observed at 25 ng ml Ϫ1 , with a much lower level of expression at 50 ng ml Ϫ1 and virtually no expression at 100 ng ml Ϫ1 , most likely due to the inhibitory effects of nisin A, though pH and temperature values were also a factor (27). In another study that sought to optimize the NICE system for the expression of lysostaphin for both laboratory (1 liter) and industrialscale (3,000 liters) applications and at high cell densities, the authors noted that the addition of too much nisin was detrimental to product formation.…”
Section: Discussionmentioning
confidence: 99%
“…(2016) synthesized an AFP with the sequence GAGP[(GVGVP)(GEGVP) 9 ] 2 GWPH 6 , which showed superior recrystallization inhibition capabilities. Similarly, Zhang, Jin, Luo, Wu, and Wang (2018) synthesized an AFP (DTASDAFAAAAL) which showed both recrystallization inhibition capabilities and THA.…”
Section: Production and Purification Of Afpsmentioning
confidence: 99%
“…As shown in Table 1, AFPs generally have structural characteristics that include the presence of a tripeptide repeat sequence (‐Gly‐Pro‐X‐, ‐Gly‐X‐Pro‐, ‐Gly‐X‐X‐ or ‐T‐X‐T‐), GTPG‐, GPP(OH)G‐, Pro‐Ala‐Gly‐Tyr, and rich in Ala. It has been found that the antifreeze activities of antifreeze proteins or ice structure proteins are closely related to their amino acid content, particularly Gly, Ala, and Thr (Damodaran & Wang, 2017; Duman & DeVries, 1972; Wang et al., 2014; Zhang et al., 2018). Therefore, one way to improve the activity of AFPs is to increase the content of these amino acids.…”
Section: Molecular Characteristics and Structure–function Relationshimentioning
confidence: 99%
“…Developing this approach, we came up with a much more efficient method of fighting the multidrug resistant bacteria. According to our method [38], in addition to the tetracycline-resistant gene, the interference plasmid contains the antibiotic A encoding gene and ligand-dependent transcription factor sequence linked to a promoter that is known in the art [39][40][41][42]. Since the transcription factor for the antibiotic A encoding gene is ligand-dependent, the synthesis of antibiotic A is switched off in the presence of a ligand (small molecule M1) and switched on in the absence of the ligand.…”
Section: Hypothesismentioning
confidence: 99%