Intracellular expression profiling by laser capture microdissection: three novel components of the neuromuscular junction

Nazarian, Javad; Bouri, Khaled; Hoffman, Eric P.

doi:10.1152/physiolgenomics.00227.2004

Cited by 36 publications

(30 citation statements)

References 53 publications

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“…6C). This result provides direct evidence (in addition to that provided by expression arrays) that nestin mRNA is confined to the nmj (Kishi et al, 2005;McGeachie et al, 2005;Nazarian et al, 2005) and that nestin is transcribed in a synapse-specific manner.…”

Section: Nestin Mrna Is Localized To the Postsynaptic Apparatus In Mumentioning

confidence: 54%

Regulation of the Intermediate Filament Protein Nestin at Rodent Neuromuscular Junctions by Innervation and Activity

Kang

Tian²,

Son³

et al. 2007

J. Neurosci.

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The intermediate filament nestin is localized postsynaptically at rodent neuromuscular junctions. The protein forms a filamentous network beneath and between the synaptic gutters, surrounds myofiber nuclei, and is associated with Z-discs adjacent to the junction. In situ hybridization shows that nestin mRNA is synthesized selectively by synaptic myonuclei. Although weak immunoreactivity is present in myelinating Schwann cells that wrap the preterminal axon, nestin is not detected in the terminal Schwann cells (tSCs) that cover the nerve terminal branches. However, after denervation of muscle, nestin is upregulated in tSCs and in SCs within the nerve distal to the lesion site. In contrast, immunoreactivity is strongly downregulated in the muscle fiber. Transgenic mice in which the nestin neural enhancer drives expression of a green fluorescent protein (GFP) reporter show that the regulation in SCs is transcriptional. However, the postsynaptic expression occurs through enhancer elements distinct from those responsible for regulation in SCs. Application of botulinum toxin shows that the upregulation in tSCs and the loss of immunoreactivity in muscle fibers occurs with blockade of transmitter release. Extrinsic stimulation of denervated muscle maintains the postsynaptic expression of nestin but does not affect the upregulation in SCs. Thus, a nestin-containing cytoskeleton is promoted in the postsynaptic muscle fiber by nerve-evoked muscle activity but suppressed in tSCs by transmitter release. Nestin antibodies and GFP driven by nestin promoter elements serve as excellent markers for the reactive state of SCs. Vital imaging of GFP shows that SCs grow a dynamic set of processes after denervation.

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Section: Nestin Mrna Is Localized To the Postsynaptic Apparatus In Mumentioning

confidence: 54%

Regulation of the Intermediate Filament Protein Nestin at Rodent Neuromuscular Junctions by Innervation and Activity

Kang

Tian²,

Son³

et al. 2007

J. Neurosci.

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“…This phenomenon of nestin 'lateralization' observed in maturing myotubes and myofibers could represent a mechanism how nestin is eliminated from the differentiating myofiber. During skeletal muscle development nestin is down-regulated and in adult myofibers it is expressed only in neuromuscular and myotendinous junctions [Carlsson et al, 1999;Vaittinen et al, 1999;Nazarian et al, 2005;Kang et al, 2007]. In the mature myofibers of recipients we also noticed areas corresponding to neuromuscular junctions exhibiting strong nestin signal in the focal protrusions of the sarcoplasm.…”

Section: Expression Of Nestin In Myogenic Elementsmentioning

confidence: 57%

Nestin Expression Reflects Formation, Revascularization and Reinnervation of New Myofibers in Regenerating Rat Hind Limb Skeletal Muscles

Čížková

Soukup²,

Mokrý³

2008

Cells Tissues Organs

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This work provides a comprehensive view of expression and distribution patterns of the intermediate filament nestin in rat regenerating skeletal muscles. Regeneration was induced by heterochronous isotransplantation of extensor digitorum longus (EDL) or soleus muscles from 15-day-old rats into the EDL muscle of adult female inbred Lewis rats. Recipients were sacrificed by anesthetic overdose and the host muscles with the graft were excised after 7-, 16-, 21- and 29-day survival, fixed in 10% formalin solution and immunohistochemically stained. In the regenerating skeletal muscle maximal nestin expression was observed in newly formed myoblasts and myotubes and it decreased in the course of skeletal myofiber development. We demonstrate the phenomenon of a peripheral concentration of nestin expression within the differentiating myofibers called ‘lateralization’, which may represent a mechanism of nestin elimination during the development of skeletal myofibers. Furthermore, nestin has been detected in newly formed endothelial cells of blood vessels growing into the regenerated area and also in peripheral nerves innervating the graft as well as the host muscle. In conclusion, according to our results, nestin represents an ideal marker for skeletal muscle regeneration, as its expression reflects processes of development, revascularization and reinnervation of new myofibers.

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“…88,89 Interestingly, mutation or depletion of LmnA leads to aberrant and fragile NMJs with nuclei not clustered under the NMJ. 90 In the mouse embryo, at E11, fusion between muscle progenitors leads to myotube formation, which will become the future skeletal muscles.…”

Section: Nuclear Clusteringmentioning

confidence: 99%

Moving and positioning the nucleus in skeletal muscle – one step at a time

Cadot

Gache

Gomes

2015

Nucleus

118

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Intracellular expression profiling by laser capture microdissection: three novel components of the neuromuscular junction

Abstract: . Intracellular expression profiling by laser capture microdissection: three novel components of the neuromuscular junction.

Cited by 36 publications

References 53 publications

Regulation of the Intermediate Filament Protein Nestin at Rodent Neuromuscular Junctions by Innervation and Activity

Regulation of the Intermediate Filament Protein Nestin at Rodent Neuromuscular Junctions by Innervation and Activity

Nestin Expression Reflects Formation, Revascularization and Reinnervation of New Myofibers in Regenerating Rat Hind Limb Skeletal Muscles

Moving and positioning the nucleus in skeletal muscle – one step at a time

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