Intracellular free [Ca2+] in circulating lymphocytes of spontaneously hypertensive rats

Bruschi, G; Bruschi, Maria E.; Caroppo, Maurizio; Orlandini, Guido; Pavarani, Carlo; Cavatorta, A

doi:10.1016/0024-3205(84)90247-9

Cited by 46 publications

(16 citation statements)

References 17 publications

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“…13 Hypertensive subjects were not matched for age, but there is no evidence of an agedependent influence on leukocyte [Ca 2+ ], in this or other studies, 15 although an age-related change in [Ca 2+ ], has been reported in rats. 26 This finding contrasts with reports of elevated [Ca 2+ ], in platelets in hypertension 13 l4 but is in agreement with previous studies in mononuclear leukocytes and lymphocytes.…”

Section: V25contrasting

confidence: 57%

Leukocyte ionized calcium and sodium content and blood pressure in humans.

Bing¹,

Heagerty²,

Jackson³

et al. 1986

Hypertension

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SUMMARY The relationship between leukocyte ionized calcium concentration, sodium content, and blood pressure was studied in normotensive subjects with (n = 17) and without (n = 21) a family history of hypertension and in untreated patients with essential hypertension (n = 22). There was a highly significant correlation between intracellular ionized calcium and mean supine blood pressure (measured on the same occasion) in normal subjects with no family history of hypertension (r = + 0.73, p< 0.01). This relationship was lost in normal subjects with a family history of hypertension, and in hypertensive patients there was a nonsignificant negative correlation between intracellular ionized calcium and blood pressure ( r = +0.08 and -0.31, respectively). Intracellular ionized calcium was similar in the normotensive groups (both, 126 ± 7 nmol/L) and slightly but nonsignificantly elevated in hypertensive patients (143 ± 10 nmol/L; p = 0.09). There was no correlation between intracellular ionized calcium and sodium content in any group (r<0.1). These results indicate that while leukocyte ionized calcium in normotensive subjects with no family history of hypertension may reflect smooth muscle contractility resulting in the positive correlation between leukocyte ionized calcium and blood pressure, this relationship is lost in hypertensive patients and subjects predisposed to hypertension. This may be due to an altered relationship between leukocyte and smooth muscle calcium handling in these subjects or to non-calcium-mediated influences on blood pressure. (Hypertension 8: 483-488, 1986) KEY WORDS • cytosolic calcium • sodium • leukocyte • blood pressure

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Section: V25contrasting

confidence: 57%

Leukocyte ionized calcium and sodium content and blood pressure in humans.

Bing¹,

Heagerty²,

Jackson³

et al. 1986

Hypertension

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“…Some investigators have failed to demonstrate the existence of Na + -Ca 2+ exchange. Using quin 2, Tsien et al 15 reported that [Ca 2+ ]| in lymphocytes was unchanged with changes in extracellular sodium concentration, and Shore et al 23 and Bruschi et al 24 reported that incubation with ouabain did not affect [Ca 2+ ]j in lymphocytes. These observations may result from an inhibitory effect of quin 2 on Na + -Ca 2+ exchange.…”

Section: Discussionmentioning

confidence: 99%

Intralymphocytic sodium and free calcium and plasma renin in essential hypertension.

et al. 1988

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SUMMARY Intraceilular sodium, potassium, and free calcium concentrations were investigated in lymphocytes of 30 patients with essential hypertension and 30 normotensive controls. All subjects were placed on a diet containing 8 to 10 g of sodium chloride per day. Lymphocyte sodium concentration was higher in hypertensive patients than in normotensive controls (19.8 ± 1.8 vs 18.4 ± 1.8 mmol/kg wet weight; p < 0.01), whereas lymphocyte potassium concentration was similar in both groups. Lymphocyte free calcium concentration was also higher in hypertensive patients than in normotensive controls (134.6 ± 13.2 vs 120.2 ± 16.4 nmol/L; p < 0.01). There was a positive correlation between lymphocyte sodium and free calcium concentrations in normotensive controls, in hypertensive patients, and hi the subjects combined (r = 0.59, p < 0.01; r = 0.71, p < 0.001; and r = 0.70, p < 0.001, respectively). Lymphocyte potassium concentration was not related to lymphocyte sodium or free calcium concentration in each group. In patients with essential hypertension, intraceilular sodium and free calcium concentrations were negatively correlated with plasma renin activity (r = -0 . 6 6 , p < 0.001; r = -0.60, p < 0.001, respectively), but they were not related to age, mean blood pressure, serum electrolyte concentration, or plasma norepinephrine concentration. These results suggest that a considerable relationship exists between intraceilular sodium and free calcium in lymphocytes and that, in essential hypertension, the alteration in cellular metabolism of sodium and calcium may be linked to the renin system but not to blood pressure, age, or adrenergic activity.

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“…26 -34 Tubular calcium reabsorption was found to be increased, 26 a difference that recently was demonstrated to be independent of PTH levels, changes in ultrafilterable calcium levels, and sodium and calcium excretion rates. 34 -102 Cytosolic free Ca calcium has been shown to be increased in platelets of hypertensive men 68 and lymphocytes of SHR, 69 as previously mentioned. Disturbances in calcium fluxes and permeability have been reported in a variety of plasma membranes of the SHR, including erythrocytes, adipocytes, and myocytes.…”

Section: Membrane Defects In Calcium Regulationmentioning

confidence: 61%

“…104 Together, these data suggest that if there were no counter-regulatory mechanisms, cytosolic calcium in these tissues ought to be increased in genetic hypertension, as was found in platelets 68 and lymphocytes, 69 which, as far as we know, are the only tissues directly studied thus far. As previously mentioned, the intracellular calcium pool in adipocytes of SHR and hypertensive man has been found to be enlarged 70 " 72 and was associated with reduced endoplasmic reticulum 45 Ca uptake.…”

Section: Membrane Defects In Calcium Regulationmentioning

confidence: 98%

“…Measurements of peripheral blood elements such as platelets in hypertensive patients 68 and lymphocytes in SHR, 69 using fluorescence signals of quin 2, a calcium chelating dye, have also revealed increased intracellular free calcium concentration. More importantly, patients with borderline hypertension were found to have mildly elevated calcium levels, which were intermediate in value between the normotensive and the frankly hypertensive subjects.…”

Section: Increased Cytosolic Free Calciummentioning

confidence: 99%

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The role of calcium in genetic hypertension.

Lau¹,

Eby²

1985

Hypertension

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HE importance of the calcium ion in muscle contractility and in the optimal functions of the cardiovascular and neurological systems has been recognized since the time of Ringer. A huge body of research data has been generated investigating various facets of the control of blood pressure, including the role of calcium in the biochemistry and physiology of contractile proteins. Interests have been renewed over the past decade on the role of calcium in genetic hypertension at the molecular as well as the organ or whole organism level. Many recent observations on this subject in humans with essential hypertension and in the rat model of spontaneous hypertension are of potential scientific and clinical interest. This review is an attempt to critically analyze the pertinent available data with the following objectives in mind:1. To present the evidence for and against the role of calcium deficiency in genetic hypertension 2. To integrate the information on this subject under a working hypothesis 3. T& define the important issues that need to be resolved 4. To suggest some areas and approaches of research that may fill in the gaps of our knowledge of the potential role of calcium in the pathogenesis of genetic hypertension

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Intracellular free [Ca2+] in circulating lymphocytes of spontaneously hypertensive rats

Cited by 46 publications

References 17 publications

Leukocyte ionized calcium and sodium content and blood pressure in humans.

Leukocyte ionized calcium and sodium content and blood pressure in humans.

Intralymphocytic sodium and free calcium and plasma renin in essential hypertension.

The role of calcium in genetic hypertension.

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