Intracellular Growth and Cell Cycle Progression are Dependent on (p)ppGpp Synthetase/Hydrolase in Brucella abortus

Henst, Mathilde Van der; Carlier, Elodie; Bolle, Xavier De

doi:10.3390/pathogens9070571

Cited by 7 publications

(2 citation statements)

References 33 publications

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“…In Brucella spp., where the G1-phase cell is the primary infectious form (8), synthesis of (p)ppGpp is required for proliferation in phagocytic cells and for survival in mice (73, 74). Conversely, overproduction of (p)ppGpp results in an accumulation of G1-phase cells in the population (75). In Rhizobia spp., mutants lacking (p)ppGpp are severely impacted in their ability to enter into a symbiotic relationship with the leguminous host plant (76, 77) whereas (p)ppGpp overproduction due to nitrogen deprivation delays the G1/S phase transition (78).…”

Section: Discussionmentioning

confidence: 99%

Coupling of cell growth modulation to asymmetric division and cell cycle regulation inCaulobacter crescentus

Glenn,

Lin,

Papagiannakis

et al. 2024

Preprint

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In proliferating bacteria, growth rate is often assumed to be similar between daughter cells. However, most of our knowledge of cell growth derives from studies on symmetrically dividing bacteria. In many α-proteobacteria, asymmetric division is a normal part of the life cycle, with each division producing daughter cells with different sizes and fates. Here, we demonstrate that the functionally distinct swarmer and stalked daughter cells produced by the model α-proteobacteriumCaulobacter crescentushave different average growth rates despite sharing an identical genome and environment. The discrepancy in growth rate is due to a growth slowdown during the cell cycle stage preceding DNA replication (the G1 phase), which is predominantly associated with swarmer cell functionality. We also provide evidence that the second messenger (p)ppGpp extends the timing of the G1/swarmer cell stage by slowing growth specifically during the beginning of the cell cycle. Our data further show that cells factor the amount and rate of their growth to control the G1/S transition, allowing them to adjust the time they spend with ecologically important G1-specific traits.Significance statementBacterial growth rate modulation is generally associated with changes in genetic make-up or environmental condition. This study demonstrates that the rate of cell growth can also vary between daughter cells and across cell cycle stages under invariant and unstressed environmental conditions. This is illustrated by the asymmetrically dividing α-proteobacteriumCaulobacter crescentus, which, at each division, produces two functionally distinct daughter cells that differ in average growth rate. This growth rate difference arises from a G1 phase-specific growth slowdown mediated, in part, by the (p)ppGpp alarmone. Altogether, this study showcases the coupling of cell growth modulation to asymmetric division and cell cycle regulation, which may have implications for other α-proteobacteria given their cell cycle similarities withC. crescentus.

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Section: Discussionmentioning

confidence: 99%

Coupling of cell growth modulation to asymmetric division and cell cycle regulation inCaulobacter crescentus

Glenn,

Lin,

Papagiannakis

et al. 2024

Preprint

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“…During the first steps of Brucella trafficking, the bacteria is able to block the progression of its cell cycle, remaining at the G1 stage for several hours, before it reaches its replication niche. The work of Van der Henst et al [ 7 ] demonstrated that starvation mediated by guanosine tetra- or penta-phosphate, (p)ppGpp, is one of the factors contributing to G1 arrest observed in B. abortus infection in macrophages. Adhesion to target cells is another major step forward for bacterial invasion and replication.…”

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confidence: 99%