Intracellular killing of Brucella melitensis in human macrophages with microsphere-encapsulated gentamicin

Abstract

Altogether, these results suggest that 502H and 75:25H microspheres are suitable carriers for gentamicin targeting inside human macrophages and thus for brucellosis treatment.

Gentamicin-loaded nanoparticles show improved antimicrobial effects towards Pseudomonas aeruginosa infection

“…This is similar to the results of previously published studies entrapping gentamicin in PLGA. 12 To examine the effects of the charge of both the polymer and the drug during the formulation process, we then altered the pH of the aqueous phase from 5.0 to 7.4 and observed an approximate threefold increase in drug entrapment in PLGA nanoparticles (22.4 µg per mg PLGA, Table 1) without significant alterations to the particle diameter and zeta potential. Similarly, using an s/o/w fabrication approach, an increase in entrapment of gentamicin from 7.3 µg per mg PLGA to 21 µg per mg PLGA was observed when pH was increased from 5 to 7.4 (Table 1).…”

“…Gentamicin have been previously examined in a wide variety of nanoparticle delivery systems, including poly(lactide-co-glycolide) (PLGA), chitosan, and caroboxymethyldextran-b-poly(ethyleneglycols). [12][13][14] The application of antibiotic nanoparticle formulations has been shown to offer several advantages over conventional administration and delivery methods, including the ability for drug delivery to a specific site such as an intracellular infection. 15,16 Nanoparticles can also be exploited to facilitate sustained release of an antibiotic, minimizing dosing regimens.…”

“…With higher drug encapsulation, it is possible that further improved biological effects can be determined. 12 Therefore, in this study we developed an approach to increase gentamicin entrapment in PLGA nanoparticles. We examined the efficacy of these controlled release particles in vitro and in vivo against P. aeruginosa infection, highlighting improved biological outcomes over administration of the nonencapsulated drug.…”

Drug delivery systems for potential treatment of intracellular bacterial infections

“…One can also speculate that unspecific cell activation by the surfactant and synergistic enhancement of the antibiotic activity could also have exerted some antibacterial effect. PLGA 502H microparticles prepared by the solvent evaporation method were further used against B. melitensis infected human macrophages (91). The results demonstrated that these PLGA microparticles are efficiently captured by the macrophages and that the gentamicin released from these particles is active and can exert its bactericidal effect inside the macrophagic cells.…”

Uso de antimicrobiano nanoparticulado para o tratamento da mastite subclínica de ovelhas de corte no período seco

“…Sistemas nanoparticulados têm sido propostos para a internalização de agentes antimicrobianos no ambiente intracelular, contribuindo assim para aumentar o índice terapêutico de antimicrobianos nos locais das infecções (Lecároz et al 2006).…”