Intracellular localization of a 6-O-methyl-d-glucose containing soluble polysaccharide from Mycobacterium tuberculosis

Lornitzo, Frank A.; Goldman, Dexter S.

doi:10.1016/0304-4165(68)90286-9

Cited by 14 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although various monomethyl and dimethyl ethers of fucose, rhamnose, 6-deoxytalose, and glucose have been found as hydrolysis products of certain type-specific glycolipids of mycobacteria (3,4,10), the present report is the first identification of 3-0-methyl-mannose as a constituent of a fungal polysaccharide.…”

mentioning

confidence: 54%

Unusual Reducing Sugar from Coccidioides immitis

et al. 1970

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 54%

Unusual Reducing Sugar from Coccidioides immitis

et al. 1970

View full text Add to dashboard Cite

show abstract

“…reported the presence of a 6-0-methyl-~-glucosecontaining lipopolysaccharide in different strains of mycobacteria and undertook to determine the structure of this compound (Keller & Ballou, 1968;Saier & Ballou, 1968a, b, c ;Gray & Ballou, 1972;Forsberg et al, 1982). Lornitzo & Goldman (1968) and Kobatake et al (1981) gave a description of similar lipopolysaccharides produced by other mycobacterial strains. These molecules seem to have an important role in the regulation of fatty acid synthesis (Ilton et al, 1971) by their ability to form tight complexes with long-chain acyl-CoA derivatives (Machida & Bloch, 1973;Knoche et al, 1973;Yabusaki & Ballou, 1978).…”

Section: Introductionmentioning

confidence: 98%

Isolation and Characterization of an O-Methylglucose-containing Lipopolysaccharide Produced by Nocardia otitidis-caviarum

Pommier

Michel

1986

Microbiology

View full text Add to dashboard Cite

The low-Mr lipopolysaccharide produced by Nocardia otitidis-cavirum is composed of 6-O-methyl-D-glucose (11 mol), D-glucose (8 mol) and 3-O-methyl-D-glucose (1 mol). Glyceric acid was also found as a constituent. Methylation and periodate oxidation analyses suggested that the backbone was formed by (1----4)-linked glucose and O-methylglucose residues. Glucose (1 mol) and 6-O-methylglucose (1 mol) served as branching points. Acetic acid was the major acyl substituent esterifying glucose residues. The lipopolysaccharide was isolated from the cytoplasmic fraction. Several other Nocardia strains were examined; all possessed the same lipopolysaccharide in their extracts.

show abstract

“…3B and 4B, the disruptions of Rv3032 and MSMEG2349 had significant impacts on the MGLP patterns of M. tuberculosis and M. smegmatis. H37Rv⌬Rv3032 accumulated polysaccharidic compounds with lower masses, consisting essentially of 17 Glc residues (MGP 17,9 , MGP 17,10 , MGP 17,11 ), with lesser quantities of MGP 16,10 , MGP 18,10 , MGP 18,11 , MGP 18,12 , and MGP 19,12 . More glucosylated forms of MGLP could not be detected in this strain.…”

Section: Construction Of M Smegmatis and M Tuberculosis Glucosyltramentioning

confidence: 99%

mentioning

confidence: 99%

“…1. MMP have been found in multiple nonpathogenic fast growing species of mycobacteria (16) and in Streptomyces griseus (17), whereas MGLP have been isolated from several Nocardia species as well as M. phlei, M. smegmatis, Mycobacterium bovis BCG, M. tuberculosis, M. leprae,[13][14][15]18,19).Ballou and co-workers (2, 16, 20, 21) isolated precursors of MMP and characterized an ␣-(134)-mannosyltransferase and a 3-O-methyltransferase from cell-free extracts of M. smegmatis. These studies led to a biosynthetic model in which MMP is elongated by a linear alternating process of mannosylation followed by O-methylation, in which GDP-Man serves as the sugar donor for the mannosyltransferase and S-adenosylmethionine serves as the source of methyl groups.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Genetic Basis for the Biosynthesis of Methylglucose Lipopolysaccharides in Mycobacterium tuberculosis

Stadthagen

Sambou

Guerin

et al. 2007

Journal of Biological Chemistry

View full text Add to dashboard Cite

Mycobacteria produce two unusual polymethylated polysaccharides (PMPS), 3 the 3-O-methylmannose polysaccharides (MMP) (1-2) and the 6-O-methylglucosyl-containing lipopolysaccharides (MGLP) (3, 4). Both polysaccharides localize to the cytoplasm, where they have been postulated to regulate fatty acid synthesis by FAS-I as a consequence of their ability to form stable 1:1 complexes with long-chain fatty acids and acylcoenzyme A derivatives (5-9) (for a review, see Ref. 10). PMPS were also proposed to protect fatty acid products from degradation and to serve as general lipid carriers facilitating the synthesis of the very large and insoluble mycolic acid esters while at the same time increasing the tolerance of mycobacteria to high cytoplasmic concentrations of long-chain acyl-CoA derivatives (10 -12). PMPS were first isolated from Mycobacterium phlei, Mycobacterium smegmatis, and Mycobacterium tuberculosis in the 1960s (2-4), and much of the information we have about the structure, biosynthesis, and biological activities of these molecules comes from this early work. Others then revised the structure of MGLP and extended the analysis of these molecules to other mycobacterial species (13-15). The structures of MGLP and MMP are shown in Fig. 1. MMP have been found in multiple nonpathogenic fast growing species of mycobacteria (16) and in Streptomyces griseus (17), whereas MGLP have been isolated from several Nocardia species as well as M. phlei, M. smegmatis, Mycobacterium bovis BCG, M. tuberculosis, M. leprae,[13][14][15]18,19).Ballou and co-workers (2, 16, 20, 21) isolated precursors of MMP and characterized an ␣-(134)-mannosyltransferase and a 3-O-methyltransferase from cell-free extracts of M. smegmatis. These studies led to a biosynthetic model in which MMP is elongated by a linear alternating process of mannosylation followed by O-methylation, in which GDP-Man serves as the sugar donor for the mannosyltransferase and S-adenosylmethionine serves as the source of methyl groups. Termination of the elongation reaction occurs when the length of the chain is sufficient to confer on the polysaccharide fatty acid-binding properties (11-13 3-O-methylmannoses). At this stage, the chain is terminated with an unmethylated mannose because the acylCoA-bound oligosaccharides are no longer available as acceptors for the 3-O-methyltransferase.Knowledge of the initiation, elongation, and termination reactions involved in the biosynthesis of MGLP is more limited. A membrane-associated acyltransferase activity responsible for the transfer of acetyl, propionyl, isobutyryl, octanoyl, and succinyl groups from their respective acyl-CoA derivatives onto *

show abstract

Intracellular localization of a 6-O-methyl-d-glucose containing soluble polysaccharide from Mycobacterium tuberculosis

Cited by 14 publications

References 14 publications

Unusual Reducing Sugar from Coccidioides immitis

Unusual Reducing Sugar from Coccidioides immitis

Isolation and Characterization of an O-Methylglucose-containing Lipopolysaccharide Produced by Nocardia otitidis-caviarum

Genetic Basis for the Biosynthesis of Methylglucose Lipopolysaccharides in Mycobacterium tuberculosis

Contact Info

Product

Resources

About